(Linear) (Six-base) MAPSORT of: KIEE4465.seq Check: 2134 from: 1 to: 1461 KIEE4465.orf With 182 enzymes: SgfI * March 22, 2012 18:07 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1402 1461 Size: 1402 59 AflIII A'CryG_T Cuts at: 0 812 1461 Size: 812 649 AhdI GACnn_n'nnGTC Cuts at: 0 71 1461 Size: 71 1390 ApaI G_GGCC'C Cuts at: 0 191 317 1461 Size: 191 126 1144 Fragments arranged by size: 1144 191 126 ApoI r'AATT_y Cuts at: 0 615 1461 Size: 615 846 BamHI G'GATC_C Cuts at: 0 776 1461 Size: 776 685 BanI G'GyrC_C Cuts at: 0 121 235 637 1040 1224 1461 Size: 121 114 402 403 184 237 Fragments arranged by size: 403 402 237 184 121 114 BanII G_rGCy'C Cuts at: 0 191 317 1386 1461 Size: 191 126 1069 75 Fragments arranged by size: 1069 191 126 75 BbeI G_GCGC'C Cuts at: 0 641 1044 1461 Size: 641 403 417 Fragments arranged by size: 641 417 403 BbsI GAAGACnn'nnnn_ Cuts at: 0 1457 1461 Size: 1457 4 BbvCI CC'TCA_GC Cuts at: 0 388 1461 Size: 388 1073 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1392 1426 1461 Size: 1392 34 35 Fragments arranged by size: 1392 35 34 BciVI GTATCCnnnnn_n' Cuts at: 0 299 1461 Size: 299 1162 BclI T'GATC_A Cuts at: 0 266 1461 Size: 266 1195 BlpI GC'TnA_GC Cuts at: 0 166 1461 Size: 166 1295 Bme1580I G_kGCm'C Cuts at: 0 124 191 240 317 1229 1461 Size: 124 67 49 77 912 232 Fragments arranged by size: 912 232 124 77 67 49 BmgBI CAC'GTC Cuts at: 0 368 1461 Size: 368 1093 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1034 1461 Size: 1034 427 Bpu10I CC'TnA_GC Cuts at: 0 388 1461 Size: 388 1073 BsaAI yAC'GTr Cuts at: 0 987 1461 Size: 987 474 BsaBI GATnn'nnATC Cuts at: 0 1093 1461 Size: 1093 368 BsaHI Gr'CG_yC Cuts at: 0 638 1041 1461 Size: 638 403 420 Fragments arranged by size: 638 420 403 BsaWI w'CCGG_w Cuts at: 0 1443 1461 Size: 1443 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 319 349 1461 Size: 319 30 1112 Fragments arranged by size: 1112 319 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 428 1461 Size: 428 1033 BseYI C'CCAG_C Cuts at: 0 87 231 513 641 1461 Size: 87 144 282 128 820 Fragments arranged by size: 820 282 144 128 87 BsiHKAI G_wGCw'C Cuts at: 0 167 593 1461 Size: 167 426 868 Fragments arranged by size: 868 426 167 Bsp1286I G_dGCh'C Cuts at: 0 124 167 191 240 317 593 1229 1386 Size: 124 43 24 49 77 276 636 157 Cuts at: 1386 1461 Size: 75 Fragments arranged by size: 636 276 157 124 77 75 49 43 24 BspHI T'CATG_A Cuts at: 0 806 1461 Size: 806 655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 761 967 1461 Size: 761 206 494 Fragments arranged by size: 761 494 206 BstEII G'GTnAC_C Cuts at: 0 880 1461 Size: 880 581 BstXI CCAn_nnnn'nTGG Cuts at: 0 117 666 1461 Size: 117 549 795 Fragments arranged by size: 795 549 117 BstYI r'GATC_y Cuts at: 0 140 776 1461 Size: 140 636 685 Fragments arranged by size: 685 636 140 BtgI C'CryG_G Cuts at: 0 542 1461 Size: 542 919 EaeI y'GGCC_r Cuts at: 0 461 545 563 761 1461 Size: 461 84 18 198 700 Fragments arranged by size: 700 461 198 84 18 EarI CTCTTCn'nnn_ Cuts at: 0 337 1461 Size: 337 1124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1034 1402 1461 Size: 1034 368 59 Fragments arranged by size: 1034 368 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 301 1461 Size: 301 1160 EcoO109I rG'GnC_Cy Cuts at: 0 187 188 219 956 1461 Size: 187 1 31 737 505 Fragments arranged by size: 737 505 187 31 1 HaeII r_GCGC'y Cuts at: 0 641 1044 1461 Size: 641 403 417 Fragments arranged by size: 641 417 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 25 57 89 1099 1131 1461 Size: 25 32 32 1010 32 330 Fragments arranged by size: 1010 330 32 32 32 25 KasI G'GCGC_C Cuts at: 0 637 1040 1461 Size: 637 403 421 Fragments arranged by size: 637 421 403 MscI TGG'CCA Cuts at: 0 565 763 1461 Size: 565 198 698 Fragments arranged by size: 698 565 198 MslI CAynn'nnrTG Cuts at: 0 115 805 811 829 985 1183 1261 1461 Size: 115 690 6 18 156 198 78 200 Fragments arranged by size: 690 200 198 156 115 78 18 6 MspA1I CmG'CkG Cuts at: 0 544 581 645 970 1461 Size: 544 37 64 325 491 Fragments arranged by size: 544 491 325 64 37 NarI GG'CG_CC Cuts at: 0 638 1041 1461 Size: 638 403 420 Fragments arranged by size: 638 420 403 NdeI CA'TA_TG Cuts at: 0 202 1461 Size: 202 1259 NruI TCG'CGA Cuts at: 0 226 1461 Size: 226 1235 NspI r_CATG'y Cuts at: 0 1011 1461 Size: 1011 450 PflMI CCAn_nnn'nTGG Cuts at: 0 864 1461 Size: 864 597 PpuMI rG'GwC_Cy Cuts at: 0 219 956 1461 Size: 219 737 505 Fragments arranged by size: 737 505 219 PspOMI G'GGCC_C Cuts at: 0 187 313 1461 Size: 187 126 1148 Fragments arranged by size: 1148 187 126 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 802 834 1461 Size: 802 32 627 Fragments arranged by size: 802 627 32 PstI C_TGCA'G Cuts at: 0 854 1461 Size: 854 607 PvuII CAG'CTG Cuts at: 0 645 1461 Size: 645 816 SacII CC_GC'GG Cuts at: 0 545 1461 Size: 545 916 SfcI C'TryA_G Cuts at: 0 850 1461 Size: 850 611 SfoI GGC'GCC Cuts at: 0 639 1042 1461 Size: 639 403 419 Fragments arranged by size: 639 419 403 StuI AGG'CCT Cuts at: 0 307 1215 1461 Size: 307 908 246 Fragments arranged by size: 908 307 246 StyI C'CwwG_G Cuts at: 0 600 1461 Size: 600 861 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 223 1461 Size: 223 1238 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1166 1198 1461 Size: 1166 32 263 Fragments arranged by size: 1166 263 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1372 1461 Size: 1372 89 XmnI GAAnn'nnTTC Cuts at: 0 535 1461 Size: 535 926 Enzymes that do cut: AcuI AflIII AhdI ApaI ApoI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BstEII BstXI BstYI BtgI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NarI NdeI NruI NspI PflMI PpuMI PspOMI PsrI PstI PvuII SacII SfcI SfoI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BfrBI BglI BglII BmrI BmtI BplI BpuEI BsaI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NgoMIV NheI NotI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI