(Circular) (Six-base) MAPSORT of: pFN21AE2166.seq Check: 7417 from: 1 to: 5764 pFN21AE2166 5764 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2241 3827 5211 5479 1632 Size: 609 1586 1384 268 1917 Fragments arranged by size: 1917 1586 1384 609 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2949 4663 5640 1203 Size: 1746 1714 977 1327 Fragments arranged by size: 1746 1714 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2551 1166 Size: 1385 4379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaI G_GGCC'C Cuts at: 2756 2756 Size: 5764 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 3073 3299 3451 5560 1493 Size: 1580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2906 3089 1870 Size: 73 963 183 4545 Fragments arranged by size: 4545 963 183 73 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2382 2630 3085 4540 Size: 325 217 627 258 337 248 455 1455 Cuts at: 4540 618 Size: 1842 Fragments arranged by size: 1842 1455 627 455 337 325 258 248 217 BanII G_rGCy'C Cuts at: 1391 2142 2508 2756 3083 1391 Size: 751 366 248 327 4072 Fragments arranged by size: 4072 751 366 327 248 BbeI G_GCGC'C Cuts at: 2049 2049 Size: 5764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2767 928 Size: 971 868 3925 Fragments arranged by size: 3925 971 868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2962 2996 3955 3989 1903 Size: 34 1025 34 959 34 3678 Fragments arranged by size: 3678 1025 959 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2633 2756 2975 3001 3825 4981 1790 Size: 843 123 219 26 824 1156 2573 Fragments arranged by size: 2573 1156 843 824 219 123 26 BmgBI CAC'GTC Cuts at: 1318 2991 1318 Size: 1673 4091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 3495 4453 Size: 416 53 83 399 24 1212 866 958 Cuts at: 4453 442 Size: 1753 Fragments arranged by size: 1753 1212 958 866 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2270 2521 4424 1239 Size: 975 56 251 1903 2579 Fragments arranged by size: 2579 1903 975 251 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 3889 4754 5052 2582 Size: 1307 865 298 3294 Fragments arranged by size: 3294 1307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2332 2950 3555 493 Size: 711 1128 618 605 2702 Fragments arranged by size: 2702 1128 711 618 605 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2532 2829 3571 Size: 53 83 186 936 513 486 297 742 Cuts at: 3571 3953 4588 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 742 635 513 486 382 297 186 83 53 BsaWI w'CCGG_w Cuts at: 2262 4197 4595 4869 5016 5427 2262 Size: 1935 398 274 147 411 2599 Fragments arranged by size: 2599 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2924 1186 Size: 1738 4026 BseYI C'CCAG_C Cuts at: 2499 2626 3009 4967 5463 2499 Size: 127 383 1958 496 2800 Fragments arranged by size: 2800 1958 496 383 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2050 2510 1163 Size: 573 314 460 4417 Fragments arranged by size: 4417 573 460 314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2307 2487 3214 3975 5003 1540 Size: 275 186 306 180 727 761 1028 2301 Fragments arranged by size: 2301 1028 761 727 306 275 186 180 BsiHKAI G_wGCw'C Cuts at: 2294 2526 3825 3910 4981 2294 Size: 232 1299 85 1071 3077 Fragments arranged by size: 3077 1299 1071 232 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 BsmBI CGTCTCn'nnnn_ Cuts at: 2110 2110 Size: 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2294 2508 2526 2633 2756 2975 Size: 399 352 152 214 18 107 123 219 Cuts at: 2975 3001 3083 3825 3910 4981 1391 Size: 26 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 219 214 152 123 107 85 82 26 18 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 2112 3653 5383 2112 Size: 1541 1730 2493 Fragments arranged by size: 2493 1730 1541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2448 2520 2613 3119 844 Size: 1331 273 72 93 506 3489 Fragments arranged by size: 3489 1331 506 273 93 72 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2679 4408 4595 1700 Size: 110 869 1729 187 2869 Fragments arranged by size: 2869 1729 869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2684 3041 3824 4836 2684 Size: 357 783 1012 3612 Fragments arranged by size: 3612 1012 783 357 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 2744 1184 Size: 1560 4204 BstXI CCAn_nnnn'nTGG Cuts at: 2626 2626 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2178 2736 3094 3461 3846 3863 5315 5401 Size: 206 558 358 367 385 17 1452 86 Cuts at: 5401 5413 5759 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 558 385 367 358 346 206 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2847 2847 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2218 2824 2918 513 Size: 552 1153 606 94 3359 Fragments arranged by size: 3359 1153 606 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2999 3280 4074 4094 2999 Size: 281 794 20 4669 Fragments arranged by size: 4669 794 281 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2131 2131 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 2469 2484 Size: 54 1425 50 275 18 474 165 15 Cuts at: 2484 2805 3211 4100 5729 8 Size: 321 406 889 1629 43 Fragments arranged by size: 1629 1425 889 474 406 321 275 165 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2304 2484 3211 1537 Size: 275 492 180 727 4090 Fragments arranged by size: 4090 727 492 275 180 EarI CTCTTCn'nnn_ Cuts at: 1353 2957 3694 1353 Size: 1604 737 3423 Fragments arranged by size: 3423 1604 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2241 2270 2521 3827 4424 5211 Size: 393 582 27 29 251 1306 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 1306 787 597 582 393 268 251 29 27 EcoO109I rG'GnC_Cy Cuts at: 2752 2752 Size: 5764 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2049 2500 2680 2699 4911 1678 Size: 371 451 180 19 2212 2531 Fragments arranged by size: 2531 2212 451 371 180 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2728 2760 2975 3007 4405 Size: 32 135 32 1156 32 215 32 1398 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1398 1156 215 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KasI G'GCGC_C Cuts at: 2045 2045 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 4878 5062 1305 Size: 1248 2325 184 2007 Fragments arranged by size: 2325 2007 1248 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2551 3053 3722 4081 4240 518 Size: 648 1385 502 669 359 159 2042 Fragments arranged by size: 2042 1385 669 648 502 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2434 2761 2826 2920 3013 3180 3857 Size: 453 375 327 65 94 93 167 677 Cuts at: 3857 5005 5250 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 453 375 327 245 167 94 93 65 NaeI GCC'GGC Cuts at: 2681 2681 Size: 5764 NarI GG'CG_CC Cuts at: 2046 2046 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5212 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NgoMIV G'CCGG_C Cuts at: 2679 2679 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 2707 3020 3122 4667 1132 Size: 97 1478 313 102 1545 2229 Fragments arranged by size: 2229 1545 1478 313 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 2868 1108 Size: 1227 533 4004 Fragments arranged by size: 4004 1227 533 PfoI T'CCnGG_A Cuts at: 2247 2454 2537 2247 Size: 207 83 5474 Fragments arranged by size: 5474 207 83 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2332 2950 2332 Size: 618 5146 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspOMI G'GGCC_C Cuts at: 2752 2752 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2761 3013 2761 Size: 252 5512 SacII CC_GC'GG Cuts at: 2827 2921 2827 Size: 94 5670 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SapI GCTCTTCn'nnn_ Cuts at: 2957 2957 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 2888 4012 5724 2888 Size: 1124 1712 2928 Fragments arranged by size: 2928 1712 1124 SfcI C'TryA_G Cuts at: 826 1045 3112 4247 4928 5119 826 Size: 219 2067 1135 681 191 1471 Fragments arranged by size: 2067 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2047 2047 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3868 4769 5031 820 Size: 197 926 654 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 654 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 2707 3020 3122 1132 Size: 1575 313 102 3774 Fragments arranged by size: 3774 1575 313 102 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2852 1517 Size: 1335 4429 StyI C'CwwG_G Cuts at: 513 1065 2051 2142 3203 513 Size: 552 986 91 1061 3074 Fragments arranged by size: 3074 1061 986 552 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2648 3791 3808 3961 4146 4591 325 Size: 1255 1068 1143 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1143 1068 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2886 3057 4010 Size: 274 80 33 51 1443 909 171 953 Cuts at: 4010 5722 96 Size: 1712 138 Fragments arranged by size: 1712 1443 953 909 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 5724 5756 2309 Size: 32 3383 32 2317 Fragments arranged by size: 3383 2317 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2800 3001 2800 Size: 201 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI