(Circular) (Six-base) MAPSORT of: pFN21AB4899.seq Check: 710 from: 1 to: 6112 pFN21AB4899 6112 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3305 3922 278 Size: 53 83 186 936 1769 617 2468 Fragments arranged by size: 2468 1769 936 617 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2248 4175 5559 5827 1632 Size: 616 1927 1384 268 1917 Fragments arranged by size: 1927 1917 1384 616 268 AfeI AGC'GCT Cuts at: 3314 3314 Size: 6112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 3318 5011 5988 1203 Size: 2115 1693 977 1327 Fragments arranged by size: 2115 1693 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2419 3855 5427 2419 Size: 1436 1572 3104 Fragments arranged by size: 3104 1572 1436 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 2545 2794 3421 3647 3799 5908 1493 Size: 1052 249 627 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 627 249 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3365 3437 1870 Size: 73 1422 72 4545 Fragments arranged by size: 4545 1422 73 72 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2289 2455 2611 3433 4888 Size: 325 217 627 502 166 156 822 1455 Cuts at: 4888 618 Size: 1842 Fragments arranged by size: 1842 1455 822 627 502 325 217 166 156 BanII G_rGCy'C Cuts at: 1391 2870 3431 1391 Size: 1479 561 4072 Fragments arranged by size: 4072 1479 561 BbeI G_GCGC'C Cuts at: 2293 2615 2293 Size: 322 5790 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 2502 1548 Size: 954 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 4723 136 Size: 107 122 71 631 605 376 2675 1525 Fragments arranged by size: 2675 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 2735 3254 3529 2735 Size: 519 275 5318 Fragments arranged by size: 5318 519 275 Bme1580I G_kGCm'C Cuts at: 1790 4173 5329 1790 Size: 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3843 4801 442 Size: 416 53 83 399 24 2426 958 1753 Fragments arranged by size: 2426 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2087 2087 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2513 2867 3134 3180 3326 4772 1239 Size: 1229 45 354 267 46 146 1446 2579 Fragments arranged by size: 2579 1446 1229 354 267 146 46 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 4237 5102 5400 2057 Size: 2180 865 298 2769 Fragments arranged by size: 2769 2180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3319 3903 493 Size: 711 2115 584 2702 Fragments arranged by size: 2702 2115 711 584 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2290 2612 2819 3302 Size: 53 83 186 936 757 322 207 483 Cuts at: 3302 3390 3919 4301 4936 275 Size: 88 529 382 635 1451 Fragments arranged by size: 1451 936 757 635 529 483 382 322 207 186 88 83 53 BsaWI w'CCGG_w Cuts at: 4545 4943 5217 5364 5775 4545 Size: 398 274 147 411 4882 Fragments arranged by size: 4882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 2819 2849 1178 Size: 30 165 30 1140 30 246 30 4441 Fragments arranged by size: 4441 1140 246 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 3031 1186 Size: 1684 161 4267 Fragments arranged by size: 4267 1684 161 BseYI C'CCAG_C Cuts at: 2684 3352 5315 5811 2684 Size: 668 1963 496 2985 Fragments arranged by size: 2985 1963 668 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2245 3359 1163 Size: 573 509 1114 3916 Fragments arranged by size: 3916 1114 573 509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2870 4173 4258 5329 2870 Size: 1303 85 1071 3653 Fragments arranged by size: 3653 1303 1071 85 BsmI GAATG_Cn' Cuts at: 2298 2628 3623 3716 2298 Size: 330 995 93 4694 Fragments arranged by size: 4694 995 330 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3156 3156 Size: 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2870 3431 4173 4258 5329 1391 Size: 399 1080 561 742 85 1071 2174 Fragments arranged by size: 2174 1080 1071 742 561 399 85 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 2185 4001 5731 2185 Size: 1816 1730 2566 Fragments arranged by size: 2566 1816 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3061 3467 844 Size: 2217 406 3489 Fragments arranged by size: 3489 2217 406 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3068 3207 4756 4943 1700 Size: 110 1258 139 1549 187 2869 Fragments arranged by size: 2869 1549 1258 187 139 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 2158 794 Size: 1355 9 4748 Fragments arranged by size: 4748 1355 9 BstBI TT'CG_AA Cuts at: 2946 4916 2946 Size: 1970 4142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107 Size: 1470 367 385 17 1452 86 12 346 Cuts at: 6107 1972 Size: 1977 Fragments arranged by size: 1977 1470 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5560 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 2147 3628 4422 4442 2147 Size: 1481 794 20 3817 Fragments arranged by size: 3817 1481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3066 3559 Size: 54 1425 50 275 18 580 656 493 Cuts at: 3559 4448 6077 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 656 580 493 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 4042 1353 Size: 780 1909 3423 Fragments arranged by size: 3423 1909 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2182 4699 5073 5219 387 Size: 1064 138 593 2517 374 146 1280 Fragments arranged by size: 2517 1280 1064 593 374 146 138 EcoICRI GAG'CTC Cuts at: 2868 2868 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2248 2468 2513 2867 3134 3180 3326 Size: 393 616 220 45 354 267 46 146 Cuts at: 3326 4175 4772 5559 5827 1239 Size: 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 616 597 393 354 268 267 220 146 46 45 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 6112 EcoO109I rG'GnC_Cy Cuts at: 2106 2106 Size: 6112 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6112 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2080 2293 2615 3316 5259 1678 Size: 402 213 322 701 1943 2531 Fragments arranged by size: 2531 1943 701 402 322 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2819 2851 3107 Size: 32 135 32 943 32 272 32 256 Cuts at: 3107 3139 4753 4785 4827 4859 1373 Size: 32 1614 32 42 32 2626 Fragments arranged by size: 2626 1614 943 272 256 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2217 3456 3702 669 Size: 988 281 279 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 279 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 2217 3702 2217 Size: 1485 4627 KasI G'GCGC_C Cuts at: 2289 2611 2289 Size: 322 5790 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5226 5410 1305 Size: 3921 184 2007 Fragments arranged by size: 3921 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 10 Size: 54 1425 343 580 3710 Fragments arranged by size: 3710 1425 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4070 4429 4588 518 Size: 648 2904 359 159 2042 Fragments arranged by size: 2904 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2287 3059 3097 3153 3528 4205 5353 5598 Size: 681 772 38 56 375 677 1148 245 Cuts at: 5598 1606 Size: 2120 Fragments arranged by size: 2120 1148 772 681 677 375 245 56 38 NaeI GCC'GGC Cuts at: 3070 3070 Size: 6112 NarI GG'CG_CC Cuts at: 2290 2612 2290 Size: 322 5790 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5560 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NgoMIV G'CCGG_C Cuts at: 3068 3068 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2668 3470 5015 1132 Size: 97 1439 802 1545 2229 Fragments arranged by size: 2229 1545 1439 802 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6112 PfoI T'CCnGG_A Cuts at: 2655 2888 3111 2655 Size: 233 223 5656 Fragments arranged by size: 5656 233 223 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 2106 Size: 6112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PstI C_TGCA'G Cuts at: 830 2394 2960 3058 3464 830 Size: 1564 566 98 406 3478 Fragments arranged by size: 3478 1564 566 406 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 3059 3097 3153 3059 Size: 38 56 6018 Fragments arranged by size: 6018 56 38 SacI G_AGCT'C Cuts at: 2870 2870 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SanDI GG'GwC_CC Cuts at: 2106 2106 Size: 6112 SapI GCTCTTCn'nnn_ Cuts at: 2133 2133 Size: 6112 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SexAI A'CCwGG_T Cuts at: 2034 2034 Size: 6112 SfcI C'TryA_G Cuts at: 826 1045 2390 2956 3054 3460 4595 5276 5467 Size: 219 1345 566 98 406 1135 681 191 Cuts at: 5467 826 Size: 1471 Fragments arranged by size: 1471 1345 1135 681 566 406 219 191 98 SfoI GGC'GCC Cuts at: 2291 2613 2291 Size: 322 5790 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 3207 1700 Size: 1507 4605 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 4216 5117 5379 820 Size: 197 926 129 2144 901 262 1553 Fragments arranged by size: 2144 1553 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2428 3551 513 Size: 552 1363 1123 3074 Fragments arranged by size: 3074 1363 1123 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 2698 4139 4156 4309 4494 4939 Size: 1255 879 239 1441 17 153 185 445 Cuts at: 4939 325 Size: 1498 Fragments arranged by size: 1498 1441 1255 879 445 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2548 2575 2580 2651 2683 6072 6104 2543 Size: 5 27 5 71 32 3389 32 2551 Fragments arranged by size: 3389 2551 71 32 32 27 5 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2310 2310 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 2012 2442 4241 2012 Size: 430 1799 3883 Fragments arranged by size: 3883 1799 430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3303 3920 276 Size: 53 83 186 936 1769 617 2468 Fragments arranged by size: 2468 1769 936 617 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI SwaI