(Circular) (Six-base) MAPSORT of: pFN21AB0401.seq Check: 4724 from: 1 to: 5218 pFN21AB0401 5218 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3028 278 Size: 53 83 186 936 1492 2468 Fragments arranged by size: 2468 1492 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2561 1937 Size: 624 4594 Acc65I G'GTAC_C Cuts at: 2539 2539 Size: 5218 AclI AA'CG_TT Cuts at: 1563 3345 3718 1563 Size: 1782 373 3063 Fragments arranged by size: 3063 1782 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2319 3281 4665 4933 1632 Size: 687 962 1384 268 1917 Fragments arranged by size: 1917 1384 962 687 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5021 AflIII A'CryG_T Cuts at: 1203 4117 5094 1203 Size: 2914 977 1327 Fragments arranged by size: 2914 1327 977 AgeI A'CCGG_T Cuts at: 4049 4049 Size: 5218 AhdI GACnn_n'nnGTC Cuts at: 3947 3947 Size: 5218 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5218 AlwNI CAG_nnn'CTG Cuts at: 2297 2961 4533 2297 Size: 664 1572 2982 Fragments arranged by size: 2982 1572 664 ApaLI G'TGCA_C Cuts at: 3275 4431 3275 Size: 1156 4062 ApoI r'AATT_y Cuts at: 1493 2458 2527 2753 2905 5014 1493 Size: 965 69 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 3772 160 Size: 872 2740 1606 Fragments arranged by size: 2740 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5218 AvaI C'yCGr_G Cuts at: 1870 1943 2543 1870 Size: 73 600 4545 Fragments arranged by size: 4545 600 73 BamHI G'GATC_C Cuts at: 2548 2915 2548 Size: 367 4851 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2348 2539 3994 618 Size: 325 217 627 561 191 1455 1842 Fragments arranged by size: 1842 1455 627 561 325 217 191 BanII G_rGCy'C Cuts at: 1391 2537 1391 Size: 1146 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4247 BbvCI CC'TCA_GC Cuts at: 2046 2046 Size: 5218 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3409 3443 1903 Size: 34 1472 34 3678 Fragments arranged by size: 3678 1472 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3110 4320 3110 Size: 1210 4008 BclI T'GATC_A Cuts at: 1548 2125 1548 Size: 577 4641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3829 136 Size: 107 122 71 631 605 2157 1525 Fragments arranged by size: 2157 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5213 5213 Size: 5218 BlpI GC'TnA_GC Cuts at: 2635 2635 Size: 5218 Bme1580I G_kGCm'C Cuts at: 1790 3279 4435 1790 Size: 1489 1156 2573 Fragments arranged by size: 2573 1489 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2949 3907 442 Size: 416 53 83 399 24 1532 958 1753 Fragments arranged by size: 1753 1532 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 3878 1239 Size: 978 1661 2579 Fragments arranged by size: 2579 1661 978 Bpu10I CC'TnA_GC Cuts at: 2046 2046 Size: 5218 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2389 3343 4208 4506 2389 Size: 954 865 298 3101 Fragments arranged by size: 3101 954 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3881 882 Size: 617 2382 2219 Fragments arranged by size: 2382 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3009 493 Size: 711 1805 2702 Fragments arranged by size: 2702 1805 711 BsaBI GATnn'nnATC Cuts at: 2205 5212 2205 Size: 3007 2211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3025 3407 4042 275 Size: 53 83 186 936 1492 382 635 1451 Fragments arranged by size: 1492 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3651 4049 4323 4470 4881 3651 Size: 398 274 147 411 3988 Fragments arranged by size: 3988 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4993 Fragments arranged by size: 4993 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5218 BseYI C'CCAG_C Cuts at: 4421 4917 4421 Size: 496 4722 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4645 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2668 3429 4457 1540 Size: 275 186 667 761 1028 2301 Fragments arranged by size: 2301 1028 761 667 275 186 BsiHKAI G_wGCw'C Cuts at: 3279 3364 4435 3279 Size: 85 1071 4062 Fragments arranged by size: 4062 1071 85 BsmI GAATG_Cn' Cuts at: 2729 2822 2729 Size: 93 5125 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 3279 3364 4435 1391 Size: 399 747 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 85 BspEI T'CCGG_A Cuts at: 4881 4881 Size: 5218 BspHI T'CATG_A Cuts at: 3107 4837 3107 Size: 1730 3488 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2573 844 Size: 1729 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3105 1394 Size: 429 1282 3507 Fragments arranged by size: 3507 1282 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3713 3887 65 Size: 1183 2465 174 1396 Fragments arranged by size: 2465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3862 4049 1700 Size: 110 2052 187 2869 Fragments arranged by size: 2869 2052 187 110 BsrGI T'GTAC_A Cuts at: 96 2427 96 Size: 2331 2887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5218 BssSI C'ACGA_G Cuts at: 3278 4290 3278 Size: 1012 4206 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 794 Size: 1376 3842 BstBI TT'CG_AA Cuts at: 4022 4022 Size: 5218 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5218 BstYI r'GATC_y Cuts at: 1972 2293 2548 2915 3300 3317 4769 4855 4867 Size: 321 255 367 385 17 1452 86 12 Cuts at: 4867 5213 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 321 255 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2388 513 Size: 552 1323 3343 Fragments arranged by size: 3343 1323 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4479 Fragments arranged by size: 4479 683 56 BtsI GCAGTG_nn' Cuts at: 2734 3528 3548 2734 Size: 794 20 4404 Fragments arranged by size: 4404 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5218 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5183 DraI TTT'AAA Cuts at: 2522 2522 Size: 5218 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4225 809 Size: 507 2909 1802 Fragments arranged by size: 2909 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2665 3554 5183 Size: 54 1425 50 275 18 835 889 1629 Cuts at: 5183 8 Size: 43 Fragments arranged by size: 1629 1425 889 835 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2665 1537 Size: 275 853 4090 Fragments arranged by size: 4090 853 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3148 1353 Size: 1795 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3805 4179 4325 387 Size: 1064 138 2216 374 146 1280 Fragments arranged by size: 2216 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2319 3281 3878 4665 4933 1239 Size: 393 585 102 962 597 787 268 1524 Fragments arranged by size: 1524 962 787 597 585 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2237 2237 Size: 5218 EcoRI G'AATT_C Cuts at: 2527 2905 2527 Size: 378 4840 FspI TGC'GCA Cuts at: 3724 3724 Size: 5218 HaeII r_GCGC'y Cuts at: 1678 2044 4365 1678 Size: 366 2321 2531 Fragments arranged by size: 2531 2321 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3859 3891 3933 3965 1373 Size: 32 135 32 2287 32 42 32 2626 Fragments arranged by size: 2626 2287 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2562 2808 669 Size: 988 281 624 246 3079 Fragments arranged by size: 3079 988 624 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5218 HpaI GTT'AAC Cuts at: 2808 2808 Size: 5218 KpnI G_GTAC'C Cuts at: 2543 2543 Size: 5218 MfeI C'AATT_G Cuts at: 2817 2817 Size: 5218 MluI A'CGCG_T Cuts at: 5094 5094 Size: 5218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2219 4332 4516 1305 Size: 914 2113 184 2007 Fragments arranged by size: 2113 2007 914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3396 Fragments arranged by size: 3396 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3176 3535 3694 518 Size: 648 2010 359 159 2042 Fragments arranged by size: 2042 2010 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2634 3311 4459 4704 1606 Size: 1028 677 1148 245 2120 Fragments arranged by size: 2120 1148 1028 677 245 NcoI C'CATG_G Cuts at: 513 1065 2388 513 Size: 552 1323 3343 Fragments arranged by size: 3343 1323 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5218 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5218 NotI GC'GGCC_GC Cuts at: 2665 2665 Size: 5218 NspI r_CATG'y Cuts at: 1132 1229 2576 4121 1132 Size: 97 1347 1545 2229 Fragments arranged by size: 2229 1545 1347 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5218 PciI A'CATG_T Cuts at: 4117 4117 Size: 5218 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5218 PmeI GTTT'AAAC Cuts at: 2522 2522 Size: 5218 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3287 3319 4800 4832 3287 Size: 32 1481 32 3673 Fragments arranged by size: 3673 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5218 PsiI TTA'TAA Cuts at: 2788 2788 Size: 5218 PstI C_TGCA'G Cuts at: 830 2570 830 Size: 1740 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5218 SalI G'TCGA_C Cuts at: 1936 2560 1936 Size: 624 4594 SbfI CC_TGCA'GG Cuts at: 2570 2570 Size: 5218 ScaI AGT'ACT Cuts at: 3466 5178 3466 Size: 1712 3506 SfcI C'TryA_G Cuts at: 826 1045 2566 3701 4382 4573 826 Size: 219 1521 1135 681 191 1471 Fragments arranged by size: 1521 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5218 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5218 SmaI CCC'GGG Cuts at: 1872 2545 1872 Size: 673 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2368 3322 4223 4485 820 Size: 197 926 425 954 901 262 1553 Fragments arranged by size: 1553 954 926 901 425 262 197 SnaBI TAC'GTA Cuts at: 493 3009 493 Size: 2516 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5218 SphI G_CATG'C Cuts at: 1132 2576 1132 Size: 1444 3774 SspI AAT'ATT Cuts at: 5 52 3142 5 Size: 47 3090 2081 Fragments arranged by size: 3090 2081 47 StuI AGG'CCT Cuts at: 1517 2111 1517 Size: 594 4624 StyI C'CwwG_G Cuts at: 513 1065 2388 2657 513 Size: 552 1323 269 3074 Fragments arranged by size: 3074 1323 552 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3245 3262 3415 3600 4045 325 Size: 1255 1665 17 153 185 445 1498 Fragments arranged by size: 1665 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 3464 5176 Size: 274 80 33 51 1443 450 1037 1712 Cuts at: 5176 96 Size: 138 Fragments arranged by size: 1712 1443 1037 450 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5178 5210 5178 Size: 32 5186 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5218 XbaI T'CTAG_A Cuts at: 2209 2554 2209 Size: 345 4873 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5218 XmaI C'CCGG_G Cuts at: 1870 2543 1870 Size: 673 4545 XmnI GAAnn'nnTTC Cuts at: 3347 3347 Size: 5218 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3026 276 Size: 53 83 186 936 1492 2468 Fragments arranged by size: 2468 1492 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI