Restriction map for
pFN21AE3601
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Cac8I Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeIII HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MwoI NaeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 16 CGCG CG^CG
AcyI 73 GGCGCC G[GA]^CG[CT]C
AfaI 492 GTAC GT^AC
AlwNI 109 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 228 CCTGAGG CC^T[ACGT]AGG
AspHI 460 GAGCTC G[AT]GC[AT]^C
AvaI 80 CCCGGG C^[CT]CG[GA]G
BanI 73 GGCGCC G^G[CT][GA]CC
BbeI 73 GGCGCC GGCGC^C
BbsI 223 GAAGAC GAAGAC(2/6)
BlpI 58 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 58 GCTGAGC GC^T[ACGT]AGC
BpuAI 223 GAAGAC GAAGAC(2/6)
BsaHI 73 GGCGCC G[GA]^CG[CT]C
BsaWI 87 TCCGGT [AT]^CCGG[AT]
BsiHKAI 460 GAGCTC G[AT]GC[AT]^C
BsoBI 80 CCCGGG C^[CT]CG[GA]G
BspHI 125 TCATGA T^CATGA
BsrBI 66 GAGCGG CCGCTC(-3/-3)
BsrI 257 CCAGT ACTGG(1/-1)
BstEII 519 GGTCACC G^GT[ACGT]ACC
BstPI 519 GGTCACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
Bsu36I 228 CCTGAGG CC^T[ACGT]AGG
CelII 58 GCTGAGC GC^T[ACGT]AGC
DsaI 198 CCATGG C^C[GA][CT]GG
Eco81I 228 CCTGAGG CC^T[ACGT]AGG
EcoO65I 519 GGTCACC G^GT[ACGT]ACC
EcoT14I 198 CCATGG C^C[AT][AT]GG
FauI 68 GCGGG CCCGC(4/6)
HaeII 73 GGCGCC [GA]GCGC^[CT]
HgaI 35 GCGTC GACGC(5/10)
Hin1I 73 GGCGCC G[GA]^CG[CT]C
HinfI 163 GAATC G^A[ACGT]TC
KasI 73 GGCGCC G^GCGCC
MaeII 317 ACGT A^CGT
MboII 223 GAAGA GAAGA(8/7)
MseI 485 TTAA T^TAA
MspA1I 206 CAGCTG C[AC]G^C[GT]G
MvnI 16 CGCG CG^CG
NarI 73 GGCGCC GG^CGCC
NcoI 198 CCATGG C^CATGG
NdeI 336 CATATG CA^TATG
NspI 450 ACATGC [GA]CATG^[CT]
PvuII 206 CAGCTG CAG^CTG
RcaI 125 TCATGA T^CATGA
RsaI 492 GTAC GT^AC
SacI 460 GAGCTC GAGCT^C
SfcI 103 CTACAG C^T[GA][CT]AG
SfoI 73 GGCGCC GGC^GCC
SmaI 80 CCCGGG CCC^GGG
StyI 198 CCATGG C^C[AT][AT]GG
TfiI 163 GAATC G^A[AT]TC
Tru9I 485 TTAA T^TAA
Tsp45I 520 GTCAC ^GT[GC]AC
XcmI 198 CCATGGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BanII 460 GAGCTC G[GA]GC[CT]^C
532 GAGCCC
BbvI 205 GCAGC GCAGC(8/12)
454 GCTGC
BcnI 80 CCCGG CC^[GC]GG
81 CCGGG
BmyI 460 GAGCTC G[AGT]GC[ACT]^C
532 GAGCCC
BsmFI 190 GGGAC GGGAC(10/14)
437 GTCCC
Bsp1286I 460 GAGCTC G[AGT]GC[ACT]^C
532 GAGCCC
BspMI 494 ACCTGC ACCTGC(4/8)
516 GCAGGT
CfoI 74 GCGC GCG^C
277 GCGC
DdeI 59 CTGAG C^T[ACGT]AG
229 CTGAG
HapII 81 CCGG C^CGG
88 CCGG
HhaI 74 GCGC GCG^C
277 GCGC
HinP1I 74 GCGC G^CGC
277 GCGC
HpaII 81 CCGG C^CGG
88 CCGG
HphI 309 TCACC GGTGA(8/7)
521 TCACC
MaeIII 332 GTAAC ^GT[ACGT]AC
520 GTCAC
MspI 81 CCGG C^CGG
88 CCGG
NciI 80 CCCGG CC^[GC]GG
81 CCGGG
TseI 205 GCAGC G^C[AT]GC
454 GCTGC
TspRI 258 CAGTG CA[GC]TG[ACGT][ACGT]^
284 CAGTG
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AvaII BfaI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I EcoRII Fnu4HI FokI ItaI
MaeI MnlI MvaI NlaIII NlaIV
Sau96I ScrFI