################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI Ksp632I KspI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 24 CGCG CG^CG AcsI 1233 AAATTC [GA]^AATT[CT] AcyI 933 GGCGCC G[GA]^CG[CT]C AflIII 865 ACGTGT A^C[GA][CT]GT ApoI 1233 AAATTC [GA]^AATT[CT] BalI 1225 TGGCCA TGG^CCA BbeI 933 GGCGCC GGCGC^C BcgI 509 CGACCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 579 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1347 AGATCT A^GATCT BsaAI 1497 CACGTA [CT]AC^GT[GA] BsaBI 1440 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 933 GGCGCC G[GA]^CG[CT]C BsaWI 224 ACCGGA [AT]^CCGG[AT] BseRI 525 GAGGAG GAGGAG(10/8) BsgI 869 GTGCAG GTGCAG(16/14) BspMI 572 ACCTGC ACCTGC(4/8) BsrFI 1132 GCCGGC [GA]^CCGG[CT] BssSI 552 CTCGTG CACGAG(-5/-1) BstUI 24 CGCG CG^CG BstYI 1347 AGATCT [GA]^GATC[CT] Cfr10I 1132 GCCGGC [GA]^CCGG[CT] DrdI 84 GACATCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1068 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 1131 GGCCGGCC GGCCGG^CC HaeII 933 GGCGCC [GA]GCGC^[CT] HgaI 1020 GACGC GACGC(5/10) Hin1I 933 GGCGCC G[GA]^CG[CT]C HincII 300 GTCAAC GT[CT]^[GA]AC HindII 300 GTCAAC GT[CT]^[GA]AC KasI 933 GGCGCC G^GCGCC MamI 1440 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1347 AGATCT [GA]^GATC[CT] MluNI 1225 TGGCCA TGG^CCA MscI 1225 TGGCCA TGG^CCA MvnI 24 CGCG CG^CG NaeI 1132 GCCGGC GCC^GGC NarI 933 GGCGCC GG^CGCC NcoI 537 CCATGG C^CATGG NgoMIV 1132 GCCGGC G^CCGGC NspI 139 ACATGC [GA]CATG^[CT] PaeR7I 1343 CTCGAG C^TCGAG PvuII 482 CAGCTG CAG^CTG SacI 1282 GAGCTC GAGCT^C SfoI 933 GGCGCC GGC^GCC TaqI 1344 TCGA T^CGA TthHB8I 1344 TCGA T^CGA XhoI 1343 CTCGAG C^TCGAG XhoII 1347 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 327 GTAC GT^AC 1500 GTAC ApaI 1050 GGGCCC GGGCC^C 1058 GGGCCC BbvCI 1491 CCTCAGC CCTCAGC(-5/-2) 499 GCTGAGG BfaI 679 CTAG C^TAG 1007 CTAG BsaI 181 GAGACC GGTCTC(1/5) 963 GGTCTC BstEII 1395 GGTCACC G^GT[ACGT]ACC 1407 GGTGACC BstF5I 108 CATCC GGATG(2/0) 1299 GGATG BstPI 1395 GGTCACC G^GT[ACGT]ACC 1407 GGTGACC BstXI 164 CCAAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 512 CCACGGTGCTGG EcoO65I 1395 GGTCACC G^GT[ACGT]ACC 1407 GGTGACC FokI 108 CATCC GGATG(9/13) 1299 GGATG MaeI 679 CTAG C^TAG 1007 CTAG MboII 256 GAAGA GAAGA(8/7) 1521 GAAGA MslI 438 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1449 CATCACTGTG PshAI 630 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 699 GACCGTGGTC PstI 904 CTGCAG CTGCA^G 1484 CTGCAG RsaI 327 GTAC GT^AC 1500 GTAC StuI 818 AGGCCT AGG^CCT 1488 AGGCCT TfiI 945 GATTC G^A[AT]TC 1327 GAATC Tsp509I 547 AATT ^AATT 1234 AATT XcmI 405 CCAGGTGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 431 CCAACCTCACCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI Sau3AI Sau96I ScrFI SfcI SmaI SmlI StyI TseI Tsp45I TspRI Van91I XmaCI XmaI