(Circular) (Six-base) MAPSORT of: pFN21AB5918.seq Check: 3488 from: 1 to: 6301 pFN21AB5918 6301 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3098 4364 5748 6016 1632 Size: 1466 1266 1384 268 1917 Fragments arranged by size: 1917 1466 1384 1266 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2870 5200 6177 1203 Size: 1667 2330 977 1327 Fragments arranged by size: 2330 1667 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 2447 1166 Size: 1281 5020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 2714 Size: 32 6269 AlwNI CAG_nnn'CTG Cuts at: 2075 2866 3052 3073 3341 4044 5616 2075 Size: 791 186 21 268 703 1572 2760 Fragments arranged by size: 2760 1572 791 703 268 186 21 ApaI G_GGCC'C Cuts at: 3059 3067 3059 Size: 8 6293 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 3238 3610 3836 3988 6097 1493 Size: 1745 372 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 372 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2121 2430 2676 3058 3165 3284 Size: 73 111 67 309 246 382 107 119 Cuts at: 3284 3348 3626 1870 Size: 64 278 4545 Fragments arranged by size: 4545 382 309 278 246 119 111 107 73 67 64 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2359 2425 2855 2938 3362 Size: 325 217 627 572 66 430 83 424 Cuts at: 3362 3622 5077 618 Size: 260 1455 1842 Fragments arranged by size: 1842 1455 627 572 430 424 325 260 217 83 66 BanII G_rGCy'C Cuts at: 1391 2062 2502 3059 3067 3291 3620 1391 Size: 671 440 557 8 224 329 4072 Fragments arranged by size: 4072 671 557 440 329 224 8 BbeI G_GCGC'C Cuts at: 2942 2942 Size: 6301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5330 BbvCI CC'TCA_GC Cuts at: 2505 3497 2505 Size: 992 5309 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2503 2537 4492 4526 1903 Size: 34 566 34 1955 34 3678 Fragments arranged by size: 3678 1955 566 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2590 4912 136 Size: 107 122 71 631 605 918 2322 1525 Fragments arranged by size: 2322 1525 918 631 605 122 107 71 BglII A'GATC_T Cuts at: 3352 6296 3352 Size: 2944 3357 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 2428 2858 3059 3067 3365 3488 4362 5518 Size: 638 430 201 8 298 123 874 1156 Cuts at: 5518 1790 Size: 2573 Fragments arranged by size: 2573 1156 874 638 430 298 201 123 8 BmgBI CAC'GTC Cuts at: 1318 2871 3228 1318 Size: 1553 357 4391 Fragments arranged by size: 4391 1553 357 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2729 2774 2894 Size: 416 53 83 399 24 1312 45 120 Cuts at: 2894 3062 4032 4990 442 Size: 168 970 958 1753 Fragments arranged by size: 1753 1312 970 958 416 399 168 120 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2629 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2615 3149 4961 1239 Size: 1086 290 534 1812 2579 Fragments arranged by size: 2579 1812 1086 534 290 Bpu10I CC'TnA_GC Cuts at: 2505 3497 2505 Size: 992 5309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3275 3375 3377 4426 5291 5589 3275 Size: 100 2 1049 865 298 3987 Fragments arranged by size: 3987 1049 865 298 100 2 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2974 4964 882 Size: 617 681 794 1990 2219 Fragments arranged by size: 2219 1990 794 681 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 4092 493 Size: 711 2300 588 2702 Fragments arranged by size: 2702 2300 711 588 BsaBI GATnn'nnATC Cuts at: 3449 6295 3449 Size: 2846 3455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2939 4108 4490 5125 Size: 53 83 186 936 1406 1169 382 635 Cuts at: 5125 275 Size: 1451 Fragments arranged by size: 1451 1406 1169 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2229 4734 5132 5406 5553 5964 2229 Size: 2505 398 274 147 411 2566 Fragments arranged by size: 2566 2505 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2118 2148 1178 Size: 30 165 30 715 30 5331 Fragments arranged by size: 5331 715 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2545 1186 Size: 1359 4942 BseYI C'CCAG_C Cuts at: 2012 2859 3045 3066 3243 5504 6000 2012 Size: 847 186 21 177 2261 496 2313 Fragments arranged by size: 2313 2261 847 496 186 177 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2895 1163 Size: 573 1159 4569 Fragments arranged by size: 4569 1159 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3291 3389 3429 4362 4447 5518 3291 Size: 98 40 933 85 1071 4074 Fragments arranged by size: 4074 1071 933 98 85 40 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2428 2502 2858 3059 3067 3291 Size: 399 272 366 74 356 201 8 224 Cuts at: 3291 3365 3389 3429 3488 3620 4362 4447 5518 Size: 74 24 40 59 132 742 85 1071 Cuts at: 5518 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 366 356 272 224 201 132 85 74 74 59 40 24 8 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 3656 844 Size: 1742 1070 3489 Fragments arranged by size: 3489 1742 1070 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2086 2257 3278 4796 4970 65 Size: 1183 838 171 1021 1518 174 1396 Fragments arranged by size: 1518 1396 1183 1021 838 174 171 BsrFI r'CCGG_y Cuts at: 1700 1810 3137 4945 5132 1700 Size: 110 1327 1808 187 2869 Fragments arranged by size: 2869 1808 1327 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 2557 4361 5373 2557 Size: 1804 1012 3485 Fragments arranged by size: 3485 1804 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 3400 3412 1184 Size: 2216 12 4073 Fragments arranged by size: 4073 2216 12 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2524 2176 Size: 348 5953 BstYI r'GATC_y Cuts at: 1972 3352 3631 3998 4383 4400 5852 5938 5950 Size: 1380 279 367 385 17 1452 86 12 Cuts at: 5950 6296 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1380 385 367 346 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2445 2458 2517 2542 2706 513 Size: 552 939 441 13 59 25 164 4108 Fragments arranged by size: 4108 939 552 441 164 59 25 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2906 3317 3486 3817 4611 4631 2906 Size: 411 169 331 794 20 4576 Fragments arranged by size: 4576 794 411 331 169 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2095 5308 809 Size: 507 779 3213 1802 Fragments arranged by size: 3213 1802 779 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2817 3135 3139 Size: 54 1425 50 275 18 987 318 4 Cuts at: 3139 3230 3748 4637 6266 8 Size: 91 518 889 1629 43 Fragments arranged by size: 1629 1425 987 889 518 318 275 91 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4231 1353 Size: 2878 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3289 3289 Size: 6301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2325 2615 3098 3149 4364 4961 5748 Size: 393 693 290 483 51 1215 597 787 Cuts at: 5748 6016 1239 Size: 268 1524 Fragments arranged by size: 1524 1215 787 693 597 483 393 290 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 3077 3077 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2072 2680 3063 3564 2072 Size: 608 383 501 4809 Fragments arranged by size: 4809 608 501 383 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FseI GG_CCGG'CC Cuts at: 3141 3141 Size: 6301 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2942 5448 1678 Size: 1264 2506 2531 Fragments arranged by size: 2531 2506 1264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2521 2553 2798 Size: 32 135 32 716 32 201 32 245 Cuts at: 2798 2830 3196 3228 3331 3363 4942 4974 5016 Size: 32 366 32 103 32 1579 32 42 Cuts at: 5016 5048 1373 Size: 32 2626 Fragments arranged by size: 2626 1579 716 366 245 201 135 103 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2307 3645 3891 669 Size: 988 281 369 1338 246 3079 Fragments arranged by size: 3079 1338 988 369 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KasI G'GCGC_C Cuts at: 2938 2938 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5415 5599 1305 Size: 4110 184 2007 Fragments arranged by size: 4110 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3232 10 Size: 54 1425 343 1400 3079 Fragments arranged by size: 3079 1425 1400 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 3458 4259 4618 4777 518 Size: 648 1281 1011 801 359 159 2042 Fragments arranged by size: 2042 1281 1011 801 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2489 2504 2863 3523 3717 4394 5542 5787 Size: 883 15 359 660 194 677 1148 245 Cuts at: 5787 1606 Size: 2120 Fragments arranged by size: 2120 1148 883 677 660 359 245 194 15 NaeI GCC'GGC Cuts at: 3139 3139 Size: 6301 NarI GG'CG_CC Cuts at: 2939 2939 Size: 6301 NcoI C'CATG_G Cuts at: 513 1065 2004 2542 513 Size: 552 939 538 4272 Fragments arranged by size: 4272 939 552 538 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NgoMIV G'CCGG_C Cuts at: 3137 3137 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2148 3659 5204 1132 Size: 97 919 1511 1545 2229 Fragments arranged by size: 2229 1545 1511 919 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2075 2593 3052 1108 Size: 967 518 459 4357 Fragments arranged by size: 4357 967 518 459 PfoI T'CCnGG_A Cuts at: 3214 3214 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 2680 3564 2072 Size: 608 884 4809 Fragments arranged by size: 4809 884 608 PshAI GACnn'nnGTC Cuts at: 1613 2639 2708 1613 Size: 1026 69 5206 Fragments arranged by size: 5206 1026 69 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 3055 3063 3055 Size: 8 6293 PstI C_TGCA'G Cuts at: 830 2913 3493 3653 830 Size: 2083 580 160 3478 Fragments arranged by size: 3478 2083 580 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2489 2489 Size: 6301 SacI G_AGCT'C Cuts at: 3291 3291 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SanDI GG'GwC_CC Cuts at: 3564 3564 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2909 2926 3489 3649 4784 5465 5656 Size: 219 1864 17 563 160 1135 681 191 Cuts at: 5656 826 Size: 1471 Fragments arranged by size: 1864 1471 1135 681 563 219 191 160 17 SfoI GGC'GCC Cuts at: 2940 2940 Size: 6301 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 2123 2432 2678 3167 3628 1872 Size: 251 309 246 489 461 4545 Fragments arranged by size: 4545 489 461 309 251 246 SmlI C'TyrA_G Cuts at: 820 1017 1943 3290 3348 3356 3390 4405 5306 Size: 197 926 1347 58 8 34 1015 901 Cuts at: 5306 5568 820 Size: 262 1553 Fragments arranged by size: 1553 1347 1015 926 901 262 197 58 34 8 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 2825 3495 1517 Size: 1308 670 4323 Fragments arranged by size: 4323 1308 670 StyI C'CwwG_G Cuts at: 513 1065 2004 2542 2587 2820 3018 3740 513 Size: 552 939 538 45 233 198 722 3074 Fragments arranged by size: 3074 939 722 552 538 233 198 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 2996 4328 4345 4498 4683 5128 Size: 1255 1293 123 1332 17 153 185 445 Cuts at: 5128 325 Size: 1498 Fragments arranged by size: 1498 1332 1293 1255 445 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 4547 6259 Size: 274 80 33 51 1443 354 2216 1712 Cuts at: 6259 96 Size: 138 Fragments arranged by size: 2216 1712 1443 354 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2031 2063 2118 2150 6261 6293 2031 Size: 32 55 32 4111 32 2039 Fragments arranged by size: 4111 2039 55 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2417 2443 2417 Size: 26 6275 XhoI C'TCGA_G Cuts at: 1943 3348 1943 Size: 1405 4896 XmaI C'CCGG_G Cuts at: 1870 2121 2430 2676 3165 3626 1870 Size: 251 309 246 489 461 4545 Fragments arranged by size: 4545 489 461 309 251 246 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI