(Linear) (Six-base) MAPSORT of: KIBB5918.seq Check: 7271 from: 1 to: 1596 KIBB5918.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1093 1596 Size: 1093 503 AflIII A'CryG_T Cuts at: 0 865 1596 Size: 865 731 AleI CACnn'nnGTG Cuts at: 0 442 1596 Size: 442 1154 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 709 741 1596 Size: 709 32 855 Fragments arranged by size: 855 709 32 AlwNI CAG_nnn'CTG Cuts at: 0 70 861 1047 1068 1336 1596 Size: 70 791 186 21 268 260 Fragments arranged by size: 791 268 260 186 70 21 ApaI G_GGCC'C Cuts at: 0 1054 1062 1596 Size: 1054 8 534 Fragments arranged by size: 1054 534 8 ApoI r'AATT_y Cuts at: 0 1233 1596 Size: 1233 363 AvaI C'yCGr_G Cuts at: 0 49 116 425 671 1053 1160 1279 1343 Size: 49 67 309 246 382 107 119 64 Cuts at: 1343 1596 Size: 253 Fragments arranged by size: 382 309 253 246 119 107 67 64 49 BanI G'GyrC_C Cuts at: 0 354 420 850 933 1357 1596 Size: 354 66 430 83 424 239 Fragments arranged by size: 430 424 354 239 83 66 BanII G_rGCy'C Cuts at: 0 57 497 1054 1062 1286 1596 Size: 57 440 557 8 224 310 Fragments arranged by size: 557 440 310 224 57 8 BbeI G_GCGC'C Cuts at: 0 937 1596 Size: 937 659 BbvCI CC'TCA_GC Cuts at: 0 500 1492 1596 Size: 500 992 104 Fragments arranged by size: 992 500 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 498 532 1596 Size: 498 34 1064 Fragments arranged by size: 1064 498 34 BglI GCCn_nnn'nGGC Cuts at: 0 585 1596 Size: 585 1011 BglII A'GATC_T Cuts at: 0 1347 1596 Size: 1347 249 Bme1580I G_kGCm'C Cuts at: 0 423 853 1054 1062 1360 1483 1596 Size: 423 430 201 8 298 123 113 Fragments arranged by size: 430 423 298 201 123 113 8 BmgBI CAC'GTC Cuts at: 0 866 1223 1596 Size: 866 357 373 Fragments arranged by size: 866 373 357 BmrI ACTGGGnnnn_n' Cuts at: 0 724 769 889 1057 1596 Size: 724 45 120 168 539 Fragments arranged by size: 724 539 168 120 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 624 1596 Size: 624 972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 610 1144 1596 Size: 320 290 534 452 Fragments arranged by size: 534 452 320 290 Bpu10I CC'TnA_GC Cuts at: 0 500 1492 1596 Size: 500 992 104 Fragments arranged by size: 992 500 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1270 1370 1372 1596 Size: 1270 100 2 224 Fragments arranged by size: 1270 224 100 2 BsaI GGTCTCn'nnnn_ Cuts at: 0 175 969 1596 Size: 175 794 627 Fragments arranged by size: 794 627 175 BsaAI yAC'GTr Cuts at: 0 1499 1596 Size: 1499 97 BsaBI GATnn'nnATC Cuts at: 0 1444 1596 Size: 1444 152 BsaHI Gr'CG_yC Cuts at: 0 934 1596 Size: 934 662 BsaWI w'CCGG_w Cuts at: 0 224 1596 Size: 224 1372 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 113 143 1596 Size: 113 30 1453 Fragments arranged by size: 1453 113 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 540 1596 Size: 540 1056 BseYI C'CCAG_C Cuts at: 0 7 854 1040 1061 1238 1596 Size: 7 847 186 21 177 358 Fragments arranged by size: 847 358 186 177 21 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 890 1596 Size: 890 706 BsiHKAI G_wGCw'C Cuts at: 0 1286 1384 1424 1596 Size: 1286 98 40 172 Fragments arranged by size: 1286 172 98 40 Bsp1286I G_dGCh'C Cuts at: 0 57 423 497 853 1054 1062 1286 1360 Size: 57 366 74 356 201 8 224 74 Cuts at: 1360 1384 1424 1483 1596 Size: 24 40 59 113 Fragments arranged by size: 366 356 224 201 113 74 74 59 57 40 24 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 581 1596 Size: 581 1015 BsrDI GCAATG_nn' Cuts at: 0 81 252 1273 1596 Size: 81 171 1021 323 Fragments arranged by size: 1021 323 171 81 BsrFI r'CCGG_y Cuts at: 0 1132 1596 Size: 1132 464 BssSI C'ACGA_G Cuts at: 0 552 1596 Size: 552 1044 BstEII G'GTnAC_C Cuts at: 0 1395 1407 1596 Size: 1395 12 189 Fragments arranged by size: 1395 189 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 519 1596 Size: 171 348 1077 Fragments arranged by size: 1077 348 171 BstYI r'GATC_y Cuts at: 0 1347 1596 Size: 1347 249 BtgI C'CryG_G Cuts at: 0 440 453 512 537 701 1596 Size: 440 13 59 25 164 895 Fragments arranged by size: 895 440 164 59 25 13 BtsI GCAGTG_nn' Cuts at: 0 901 1312 1481 1596 Size: 901 411 169 115 Fragments arranged by size: 901 411 169 115 DrdI GACnn_nn'nnGTC Cuts at: 0 90 1596 Size: 90 1506 EaeI y'GGCC_r Cuts at: 0 812 1130 1134 1225 1596 Size: 812 318 4 91 371 Fragments arranged by size: 812 371 318 91 4 EcoICRI GAG'CTC Cuts at: 0 1284 1596 Size: 1284 312 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 610 1093 1144 1596 Size: 320 290 483 51 452 Fragments arranged by size: 483 452 320 290 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 1072 1596 Size: 1072 524 EcoO109I rG'GnC_Cy Cuts at: 0 67 675 1058 1559 1596 Size: 67 608 383 501 37 Fragments arranged by size: 608 501 383 67 37 FseI GG_CCGG'CC Cuts at: 0 1136 1596 Size: 1136 460 HaeII r_GCGC'y Cuts at: 0 937 1596 Size: 937 659 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 283 315 516 548 793 825 1191 1223 Size: 283 32 201 32 245 32 366 32 Cuts at: 1223 1326 1358 1596 Size: 103 32 238 Fragments arranged by size: 366 283 245 238 201 103 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 302 1596 Size: 302 1294 KasI G'GCGC_C Cuts at: 0 933 1596 Size: 933 663 MscI TGG'CCA Cuts at: 0 1227 1596 Size: 1227 369 MslI CAynn'nnrTG Cuts at: 0 442 1453 1596 Size: 442 1011 143 Fragments arranged by size: 1011 442 143 MspA1I CmG'CkG Cuts at: 0 484 499 858 1518 1596 Size: 484 15 359 660 78 Fragments arranged by size: 660 484 359 78 15 NaeI GCC'GGC Cuts at: 0 1134 1596 Size: 1134 462 NarI GG'CG_CC Cuts at: 0 934 1596 Size: 934 662 NcoI C'CATG_G Cuts at: 0 537 1596 Size: 537 1059 NgoMIV G'CCGG_C Cuts at: 0 1132 1596 Size: 1132 464 NspI r_CATG'y Cuts at: 0 143 1596 Size: 143 1453 PflMI CCAn_nnn'nTGG Cuts at: 0 70 588 1047 1596 Size: 70 518 459 549 Fragments arranged by size: 549 518 459 70 PfoI T'CCnGG_A Cuts at: 0 1209 1596 Size: 1209 387 PpuMI rG'GwC_Cy Cuts at: 0 67 675 1559 1596 Size: 67 608 884 37 Fragments arranged by size: 884 608 67 37 PshAI GACnn'nnGTC Cuts at: 0 634 703 1596 Size: 634 69 893 Fragments arranged by size: 893 634 69 PspOMI G'GGCC_C Cuts at: 0 1050 1058 1596 Size: 1050 8 538 Fragments arranged by size: 1050 538 8 PstI C_TGCA'G Cuts at: 0 908 1488 1596 Size: 908 580 108 Fragments arranged by size: 908 580 108 PvuII CAG'CTG Cuts at: 0 484 1596 Size: 484 1112 SacI G_AGCT'C Cuts at: 0 1286 1596 Size: 1286 310 SanDI GG'GwC_CC Cuts at: 0 1559 1596 Size: 1559 37 SfcI C'TryA_G Cuts at: 0 904 921 1484 1596 Size: 904 17 563 112 Fragments arranged by size: 904 563 112 17 SfoI GGC'GCC Cuts at: 0 935 1596 Size: 935 661 SmaI CCC'GGG Cuts at: 0 118 427 673 1162 1596 Size: 118 309 246 489 434 Fragments arranged by size: 489 434 309 246 118 SmlI C'TyrA_G Cuts at: 0 1285 1343 1351 1385 1596 Size: 1285 58 8 34 211 Fragments arranged by size: 1285 211 58 34 8 StuI AGG'CCT Cuts at: 0 820 1490 1596 Size: 820 670 106 Fragments arranged by size: 820 670 106 StyI C'CwwG_G Cuts at: 0 537 582 815 1013 1596 Size: 537 45 233 198 583 Fragments arranged by size: 583 537 233 198 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 868 991 1596 Size: 868 123 605 Fragments arranged by size: 868 605 123 TatI w'GTAC_w Cuts at: 0 326 1596 Size: 326 1270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 26 58 113 145 1596 Size: 26 32 55 32 1451 Fragments arranged by size: 1451 55 32 32 26 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 412 438 1596 Size: 412 26 1158 Fragments arranged by size: 1158 412 26 XhoI C'TCGA_G Cuts at: 0 1343 1596 Size: 1343 253 XmaI C'CCGG_G Cuts at: 0 116 425 671 1160 1596 Size: 116 309 246 489 436 Fragments arranged by size: 489 436 309 246 116 Enzymes that do cut: AcuI AflIII AleI AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BlpI BmtI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI EciI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI