################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AgeI AhdI AluI AlwI AlwNI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI HindIII HinfI HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfcI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 82 GAATTT [GA]^AATT[CT] AcyI 34 GGCGCC G[GA]^CG[CT]C AflII 268 CTTAAG C^TTAAG AflIII 47 ACGTGT A^C[GA][CT]GT Alw44I 466 GTGCAC G^TGCAC AocI 283 CCTGAGG CC^T[ACGT]AGG ApaLI 466 GTGCAC G^TGCAC ApoI 82 GAATTT [GA]^AATT[CT] Asp700I 289 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 466 GTGCAC G[AT]GC[AT]^C BbeI 34 GGCGCC GGCGC^C BbvI 160 GCTGC GCAGC(8/12) BclI 206 TGATCA T^GATCA BfrI 268 CTTAAG C^TTAAG BmyI 466 GTGCAC G[AGT]GC[ACT]^C BsaHI 34 GGCGCC G[GA]^CG[CT]C BseRI 397 GAGGAG GAGGAG(10/8) BsiHKAI 466 GTGCAC G[AT]GC[AT]^C BsmAI 149 GAGAC GTCTC(1/5) BsmI 304 GCATTC GAATGC(1/-1) Bsp1286I 466 GTGCAC G[AGT]GC[ACT]^C BsrBI 275 GAGCGG CCGCTC(-3/-3) BsrI 439 ACTGG ACTGG(1/-1) BstBI 61 TTCGAA TT^CGAA BstF5I 120 GGATG GGATG(2/0) Bsu36I 283 CCTGAGG CC^T[ACGT]AGG Cac8I 375 GCAGGC GC[ACGT]^[ACGT]GC CfoI 35 GCGC GCG^C DsaI 480 CCACGG C^C[GA][CT]GG Eco81I 283 CCTGAGG CC^T[ACGT]AGG FbaI 206 TGATCA T^GATCA FokI 120 GGATG GGATG(9/13) HaeII 34 GGCGCC [GA]GCGC^[CT] HaeIII 93 GGCC GG^CC HhaI 35 GCGC GCG^C Hin1I 34 GGCGCC G[GA]^CG[CT]C HinP1I 35 GCGC G^CGC HincII 403 GTTGAC GT[CT]^[GA]AC HindII 403 GTTGAC GT[CT]^[GA]AC HphI 476 TCACC GGTGA(8/7) KasI 34 GGCGCC G^GCGCC MaeIII 436 GTGAC ^GT[ACGT]AC MslI 99 CACTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 34 GGCGCC GG^CGCC NspV 61 TTCGAA TT^CGAA SexAI 461 ACCTGGT A^CC[AT]GGT SfoI 34 GGCGCC GGC^GCC SfuI 61 TTCGAA TT^CGAA SmlI 268 CTTAAG C^T[CT][GA]AG StuI 92 AGGCCT AGG^CCT TaqI 62 TCGA T^CGA TseI 160 GCTGC G^C[AT]GC Tsp45I 436 GTGAC ^GT[GC]AC TthHB8I 62 TCGA T^CGA XcmI 111 CCAGAACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 289 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BanI 3 GGCACC G^G[CT][GA]CC 34 GGCGCC BcnI 29 CCGGG CC^[GC]GG 220 CCGGG BsiYI 72 CCAAATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 111 CCAGAACAGGG BslI 72 CCAAATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 111 CCAGAACAGGG BstNI 78 CCAGG CC^[AT]GG 462 CCTGG DpnII 142 GATC ^GATC 207 GATC EarI 21 GAAGAG CTCTTC(1/4) 487 GAAGAG EcoRII 78 CCAGG ^CC[AT]GG 462 CCTGG EcoT14I 165 CCTTGG C^C[AT][AT]GG 392 CCAAGG Fnu4HI 160 GCTGC GC^[ACGT]GC 418 GCCGC HapII 29 CCGG C^CGG 220 CCGG HpaII 29 CCGG C^CGG 220 CCGG ItaI 160 GCTGC GC^[ACGT]GC 418 GCCGC Ksp632I 21 GAAGAG CTCTTC(1/4) 487 GAAGAG MboI 142 GATC ^GATC 207 GATC MspI 29 CCGG C^CGG 220 CCGG MvaI 78 CCAGG CC^[AT]GG 462 CCTGG MwoI 28 GCCGGGGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 366 GCTGACCACGC NciI 29 CCGGG CC^[GC]GG 220 CCGGG NdeII 142 GATC ^GATC 207 GATC NlaIII 57 CATG CATG^ 105 CATG Sau3AI 142 GATC ^GATC 207 GATC StyI 165 CCTTGG C^C[AT][AT]GG 392 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AvaII BsaJI BssKI Cfr13I DdeI MaeII MboII MnlI MseI NlaIV Sau96I ScrFI Tru9I Tsp509I