Restriction map for
pFN21AE1364
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BfaI
BglI BlnI BpuAI BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AciI 401 CCGC CCGC(-3/-1)
AcsI 186 GAATTT [GA]^AATT[CT]
AflII 423 CTTAAG C^TTAAG
AgeI 80 ACCGGT A^CCGGT
ApaI 598 GGGCCC GGGCC^C
ApoI 186 GAATTT [GA]^AATT[CT]
AspHI 751 GAGCAC G[AT]GC[AT]^C
BfrI 423 CTTAAG C^TTAAG
BglII 360 AGATCT A^GATCT
BlpI 70 GCTAAGC GC^T[ACGT]AGC
BpmI 745 CTGGAG CTGGAG(16/14)
Bpu1102I 70 GCTAAGC GC^T[ACGT]AGC
BsaBI 616 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 873 GGTCTC GGTCTC(1/5)
BsaWI 80 ACCGGT [AT]^CCGG[AT]
BsiHKAI 751 GAGCAC G[AT]GC[AT]^C
BsrFI 80 ACCGGT [GA]^CCGG[CT]
BsrI 640 CCAGT ACTGG(1/-1)
BssSI 690 CTCGTG CACGAG(-5/-1)
BstAPI 357 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 257 TTCGAA TT^CGAA
CelII 70 GCTAAGC GC^T[ACGT]AGC
CfoI 286 GCGC GCG^C
Cfr10I 80 ACCGGT [GA]^CCGG[CT]
DraII 597 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 319 GACGTGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 597 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 207 ATGCAT ATGCA^T
Fnu4HI 400 GCCGC GC^[ACGT]GC
HaeII 285 GGCGCT [GA]GCGC^[CT]
HhaI 286 GCGC GCG^C
HinP1I 286 GCGC G^CGC
HindIII 374 AAGCTT A^AGCTT
ItaI 400 GCCGC GC^[ACGT]GC
MaeII 320 ACGT A^CGT
MamI 616 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 429 CCATGG C^CATGG
NsiI 207 ATGCAT ATGCA^T
NspV 257 TTCGAA TT^CGAA
PinAI 80 ACCGGT A^CCGGT
SfuI 257 TTCGAA TT^CGAA
SmaI 152 CCCGGG CCC^GGG
SmlI 423 CTTAAG C^T[CT][GA]AG
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 94 GTAC GT^AC
177 GTAC
AvaI 152 CCCGGG C^[CT]CG[GA]G
594 CTCGGG
BanII 598 GGGCCC G[GA]GC[CT]^C
699 GAGCCC
BcnI 152 CCCGG CC^[GC]GG
153 CCGGG
BseRI 696 GAGGAG GAGGAG(10/8)
748 GAGGAG
BsmAI 822 GTCTC GTCTC(1/5)
874 GTCTC
BsoBI 152 CCCGGG C^[CT]CG[GA]G
594 CTCGGG
DdeI 71 CTAAG C^T[ACGT]AG
824 CTCAG
DsaI 429 CCATGG C^C[GA][CT]GG
671 CCACGG
EcoT14I 429 CCATGG C^C[AT][AT]GG
887 CCAAGG
HaeIII 588 GGCC GG^CC
599 GGCC
HapII 81 CCGG C^CGG
153 CCGG
HpaII 81 CCGG C^CGG
153 CCGG
MseI 368 TTAA T^TAA
424 TTAA
MspI 81 CCGG C^CGG
153 CCGG
NciI 152 CCCGG CC^[GC]GG
153 CCGGG
PleI 592 GACTC GAGTC(4/5)
820 GAGTC
RsaI 94 GTAC GT^AC
177 GTAC
SapI 25 GAAGAGC GCTCTTC(1/4)
288 GCTCTTC
StyI 429 CCATGG C^C[AT][AT]GG
887 CCAAGG
TaqI 258 TCGA T^CGA
627 TCGA
TfiI 43 GATTC G^A[AT]TC
58 GATTC
Tru9I 368 TTAA T^TAA
424 TTAA
Tsp509I 187 AATT ^AATT
447 AATT
TspRI 62 CAGTG CA[GC]TG[ACGT][ACGT]^
641 CAGTG
TthHB8I 258 TCGA T^CGA
627 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI AvaII BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DpnII EarI EcoRII FokI HinfI
HphI Ksp632I MaeIII MboI MboII
MflI MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI Tsp45I XhoII