(Circular) (Six-base) MAPSORT of: pFN21AE1364.seq Check: 8705 from: 1 to: 5614 pFN21AE1364 5614 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 2406 2890 3677 5061 5329 1632 Size: 463 311 484 787 1384 268 1917 Fragments arranged by size: 1917 1384 787 484 463 311 268 AflII C'TTAA_G Cuts at: 820 1017 2428 820 Size: 197 1411 4006 Fragments arranged by size: 4006 1411 197 AflIII A'CryG_T Cuts at: 1203 4513 5490 1203 Size: 3310 977 1327 Fragments arranged by size: 3310 1327 977 AgeI A'CCGG_T Cuts at: 2085 4445 2085 Size: 2360 3254 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 2795 1166 Size: 1629 3985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AlwNI CAG_nnn'CTG Cuts at: 3357 4929 3357 Size: 1572 4042 ApaI G_GGCC'C Cuts at: 2607 2607 Size: 5614 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2191 2923 3149 3301 5410 1493 Size: 698 732 226 152 2109 1697 Fragments arranged by size: 2109 1697 732 698 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2599 2939 1870 Size: 73 214 442 340 4545 Fragments arranged by size: 4545 442 340 214 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 4390 618 Size: 325 217 627 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 325 217 BanII G_rGCy'C Cuts at: 1391 2607 2708 2933 1391 Size: 1216 101 225 4072 Fragments arranged by size: 4072 1216 225 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BfrBI ATG'CAT Cuts at: 2214 2214 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4225 136 Size: 107 122 71 631 605 2553 1525 Fragments arranged by size: 2553 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2365 5609 2365 Size: 3244 2370 BlpI GC'TnA_GC Cuts at: 2076 3031 2076 Size: 955 4659 Bme1580I G_kGCm'C Cuts at: 1790 2607 3675 4831 1790 Size: 817 1068 1156 2573 Fragments arranged by size: 2573 1156 1068 817 BmgBI CAC'GTC Cuts at: 1318 2326 1318 Size: 1008 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2639 3345 4303 Size: 416 53 83 399 24 1222 706 958 Cuts at: 4303 442 Size: 1753 Fragments arranged by size: 1753 1222 958 706 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 4274 1239 Size: 1532 1503 2579 Fragments arranged by size: 2579 1532 1503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3739 4604 4902 3739 Size: 865 298 4451 Fragments arranged by size: 4451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2884 4277 882 Size: 617 1385 1393 2219 Fragments arranged by size: 2219 1393 1385 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 2625 5608 2625 Size: 2983 2631 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2085 4047 4445 4719 4866 5277 2085 Size: 1962 398 274 147 411 2422 Fragments arranged by size: 2422 1962 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2716 2768 1186 Size: 1530 52 4032 Fragments arranged by size: 4032 1530 52 BseYI C'CCAG_C Cuts at: 2203 4817 5313 2203 Size: 2614 496 2504 Fragments arranged by size: 2614 2504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5041 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2760 3675 3760 4831 2760 Size: 915 85 1071 3543 Fragments arranged by size: 3543 1071 915 85 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 2708 2760 2933 3675 3760 4831 Size: 399 817 101 52 173 742 85 1071 Cuts at: 4831 1391 Size: 2174 Fragments arranged by size: 2174 1071 817 742 399 173 101 85 52 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2969 844 Size: 2125 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4109 4283 65 Size: 1183 2861 174 1396 Fragments arranged by size: 2861 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 4258 4445 1700 Size: 110 275 2173 187 2869 Fragments arranged by size: 2869 2173 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 2695 3674 4686 2695 Size: 979 1012 3623 Fragments arranged by size: 3623 1012 979 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4040 BstBI TT'CG_AA Cuts at: 2263 4418 2263 Size: 2155 3459 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2365 2640 2784 2865 2944 3311 3696 3713 Size: 393 275 144 81 79 367 385 17 Cuts at: 3713 5165 5251 5263 5609 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 393 385 367 346 275 144 86 81 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2434 2676 513 Size: 552 939 430 242 3451 Fragments arranged by size: 3451 939 552 430 242 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2330 4621 809 Size: 507 1014 2291 1802 Fragments arranged by size: 2291 1802 1014 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3061 3950 5579 Size: 54 1425 50 275 18 1231 889 1629 Cuts at: 5579 8 Size: 43 Fragments arranged by size: 1629 1425 1231 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2114 2300 2382 2549 2669 2707 3544 Size: 672 89 186 82 167 120 38 837 Cuts at: 3544 1353 Size: 3423 Fragments arranged by size: 3423 837 672 186 167 120 89 82 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2406 2771 2890 3677 4274 5061 Size: 393 463 311 365 119 787 597 787 Cuts at: 5061 5329 1239 Size: 268 1524 Fragments arranged by size: 1524 787 787 597 463 393 365 311 268 119 EcoO109I rG'GnC_Cy Cuts at: 2603 2603 Size: 5614 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2387 2387 Size: 5614 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 2294 4761 1678 Size: 616 2467 2531 Fragments arranged by size: 2531 2467 616 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2350 2382 2625 Size: 32 135 32 503 32 243 32 243 Cuts at: 2625 2657 2816 2848 4255 4287 4329 4361 1373 Size: 32 159 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 503 243 243 159 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 2379 748 Size: 1631 3983 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3792 Fragments arranged by size: 3792 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2400 2795 2850 3572 3931 4090 518 Size: 648 1234 395 55 722 359 159 2042 Fragments arranged by size: 2042 1234 722 648 395 359 159 55 MspA1I CmG'CkG Cuts at: 1606 3030 3707 4855 5100 1606 Size: 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2434 513 Size: 552 939 430 3693 Fragments arranged by size: 3693 939 552 430 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NsiI A_TGCA'T Cuts at: 2216 2216 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2972 4517 1132 Size: 97 1743 1545 2229 Fragments arranged by size: 2229 1743 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5614 PfoI T'CCnGG_A Cuts at: 2748 2748 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PspOMI G'GGCC_C Cuts at: 2603 2603 Size: 5614 PstI C_TGCA'G Cuts at: 830 2966 830 Size: 2136 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SapI GCTCTTCn'nnn_ Cuts at: 2025 2300 2025 Size: 275 5339 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2962 4097 4778 4969 826 Size: 219 1917 1135 681 191 1471 Fragments arranged by size: 1917 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2159 2941 1872 Size: 287 782 4545 Fragments arranged by size: 4545 782 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 2428 3718 4619 4881 820 Size: 197 926 485 1290 901 262 1553 Fragments arranged by size: 1553 1290 926 901 485 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2004 2434 2892 3053 513 Size: 552 939 430 458 161 3074 Fragments arranged by size: 3074 939 552 458 430 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2178 2585 3641 3658 3811 3996 4441 Size: 1255 598 407 1056 17 153 185 445 Cuts at: 4441 325 Size: 1498 Fragments arranged by size: 1498 1255 1056 598 445 407 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3860 5572 Size: 274 80 33 51 1443 204 1679 1712 Cuts at: 5572 96 Size: 138 Fragments arranged by size: 1712 1679 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2452 2484 5574 5606 2452 Size: 32 3090 32 2460 Fragments arranged by size: 3090 2460 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2157 2939 1870 Size: 287 782 4545 Fragments arranged by size: 4545 782 287 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI