Restriction map for pFN21AE1364
 (Circular) (Six-base) MAPSORT of: pFN21AE1364.seq  Check: 8705  from: 1  to: 5614



pFN21AE1364  5614 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:46  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3424     278 
   Size:         53      83     186     936    1888    2468

  Fragments arranged by size: 

                2468    1888     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2957    1937 
   Size:       1020    4594
 
Acc65I G'GTAC_C

Cuts at:   2935    2935 
   Size:       5614
 
AclI AA'CG_TT

Cuts at:   1563    3741    4114    1563 
   Size:       2178     373    3063

  Fragments arranged by size: 

                3063    2178     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2095    2406    2890    3677    5061    5329    1632 
   Size:        463     311     484     787    1384     268    1917

  Fragments arranged by size: 

                1917    1384     787     484     463     311     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2428     820 
   Size:        197    1411    4006

  Fragments arranged by size: 

                4006    1411     197
 
AflIII A'CryG_T

Cuts at:   1203    4513    5490    1203 
   Size:       3310     977    1327

  Fragments arranged by size: 

                3310    1327     977
 
AgeI A'CCGG_T

Cuts at:   2085    4445    2085 
   Size:       2360    3254
 
AhdI GACnn_n'nnGTC

Cuts at:   4343    4343 
   Size:       5614
 
AleI CACnn'nnGTG

Cuts at:   1166    2795    1166 
   Size:       1629    3985
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5614
 
AlwNI CAG_nnn'CTG

Cuts at:   3357    4929    3357 
   Size:       1572    4042
 
ApaI G_GGCC'C

Cuts at:   2607    2607 
   Size:       5614
 
ApaLI G'TGCA_C

Cuts at:   3671    4827    3671 
   Size:       1156    4458
 
ApoI r'AATT_y

Cuts at:   1493    2191    2923    3149    3301    5410    1493 
   Size:        698     732     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     732     698     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4168     160 
   Size:        872    3136    1606

  Fragments arranged by size: 

                3136    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5614
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2157    2599    2939    1870 
   Size:         73     214     442     340    4545

  Fragments arranged by size: 

                4545     442     340     214      73
 
BamHI G'GATC_C

Cuts at:   2944    3311    2944 
   Size:        367    5247
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2935    4390     618 
   Size:        325     217     627    1148    1455    1842

  Fragments arranged by size: 

                1842    1455    1148     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2607    2708    2933    1391 
   Size:       1216     101     225    4072

  Fragments arranged by size: 

                4072    1216     225     101
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4643
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3805    3839    1903 
   Size:         34    1868      34    3678

  Fragments arranged by size: 

                3678    1868      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3506    4716    3506 
   Size:       1210    4404
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5614
 
BfrBI ATG'CAT

Cuts at:   2214    2214 
   Size:       5614
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4225     136 
   Size:        107     122      71     631     605    2553    1525

  Fragments arranged by size: 

                2553    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2365    5609    2365 
   Size:       3244    2370
 
BlpI GC'TnA_GC

Cuts at:   2076    3031    2076 
   Size:        955    4659
 
Bme1580I G_kGCm'C

Cuts at:   1790    2607    3675    4831    1790 
   Size:        817    1068    1156    2573

  Fragments arranged by size: 

                2573    1156    1068     817
 
BmgBI CAC'GTC

Cuts at:   1318    2326    1318 
   Size:       1008    4606
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2639    3345    4303 
   Size:        416      53      83     399      24    1222     706     958

Cuts at:   4303     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1222     958     706     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5614
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2771    4274    1239 
   Size:       1532    1503    2579

  Fragments arranged by size: 

                2579    1532    1503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3739    4604    4902    3739 
   Size:        865     298    4451

  Fragments arranged by size: 

                4451     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2884    4277     882 
   Size:        617    1385    1393    2219

  Fragments arranged by size: 

                2219    1393    1385     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3405     493 
   Size:        711    2201    2702

  Fragments arranged by size: 

                2702    2201     711
 
BsaBI GATnn'nnATC

Cuts at:   2625    5608    2625 
   Size:       2983    2631
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3421    3803    4438     275 
   Size:         53      83     186     936    1888     382     635    1451

  Fragments arranged by size: 

                1888    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2085    4047    4445    4719    4866    5277    2085 
   Size:       1962     398     274     147     411    2422

  Fragments arranged by size: 

                2422    1962     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5389

  Fragments arranged by size: 

                5389     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2716    2768    1186 
   Size:       1530      52    4032

  Fragments arranged by size: 

                4032    1530      52
 
BseYI C'CCAG_C

Cuts at:   2203    4817    5313    2203 
   Size:       2614     496    2504

  Fragments arranged by size: 

                2614    2504     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5041
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3064    3825    4853    1540 
   Size:        275     186    1063     761    1028    2301

  Fragments arranged by size: 

                2301    1063    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2760    3675    3760    4831    2760 
   Size:        915      85    1071    3543

  Fragments arranged by size: 

                3543    1071     915      85
 
BsmI GAATG_Cn'

Cuts at:   3125    3218    3125 
   Size:         93    5521
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2607    2708    2760    2933    3675    3760    4831 
   Size:        399     817     101      52     173     742      85    1071

Cuts at:   4831    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     817     742     399     173     101      85
                  52
 
BspEI T'CCGG_A

Cuts at:   5277    5277 
   Size:       5614
 
BspHI T'CATG_A

Cuts at:   3503    5233    3503 
   Size:       1730    3884
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2969     844 
   Size:       2125    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3501    1394 
   Size:        429    1678    3507

  Fragments arranged by size: 

                3507    1678     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4109    4283      65 
   Size:       1183    2861     174    1396

  Fragments arranged by size: 

                2861    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2085    4258    4445    1700 
   Size:        110     275    2173     187    2869

  Fragments arranged by size: 

                2869    2173     275     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5614
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5614
 
BssSI C'ACGA_G

Cuts at:   2695    3674    4686    2695 
   Size:        979    1012    3623

  Fragments arranged by size: 

                3623    1012     979
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2368     794 
   Size:       1574    4040
 
BstBI TT'CG_AA

Cuts at:   2263    4418    2263 
   Size:       2155    3459
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5614
 
BstYI r'GATC_y

Cuts at:   1972    2365    2640    2784    2865    2944    3311    3696    3713 
   Size:        393     275     144      81      79     367     385      17

Cuts at:   3713    5165    5251    5263    5609    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     393     385     367     346     275     144
                  86      81      79      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2434    2676     513 
   Size:        552     939     430     242    3451

  Fragments arranged by size: 

                3451     939     552     430     242
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4875

  Fragments arranged by size: 

                4875     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3130    3924    3944    3130 
   Size:        794      20    4800

  Fragments arranged by size: 

                4800     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5614
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5579
 
DraI TTT'AAA

Cuts at:   2918    2918 
   Size:       5614
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2330    4621     809 
   Size:        507    1014    2291    1802

  Fragments arranged by size: 

                2291    1802    1014     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3061    3950    5579 
   Size:         54    1425      50     275      18    1231     889    1629

Cuts at:   5579       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1231     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3061    1537 
   Size:        275    1249    4090

  Fragments arranged by size: 

                4090    1249     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2025    2114    2300    2382    2549    2669    2707    3544 
   Size:        672      89     186      82     167     120      38     837

Cuts at:   3544    1353 
   Size:       3423

  Fragments arranged by size: 

                3423     837     672     186     167     120      89      82
                  38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4201    4575    4721     387 
   Size:       1064     138    2612     374     146    1280

  Fragments arranged by size: 

                2612    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2095    2406    2771    2890    3677    4274    5061 
   Size:        393     463     311     365     119     787     597     787

Cuts at:   5061    5329    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     787     787     597     463     393     365     311
                 268     119
 
EcoO109I rG'GnC_Cy

Cuts at:   2603    2603 
   Size:       5614
 
EcoRI G'AATT_C

Cuts at:   2923    3301    2923 
   Size:        378    5236
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2387    2387 
   Size:       5614
 
FspI TGC'GCA

Cuts at:   4120    4120 
   Size:       5614
 
HaeII r_GCGC'y

Cuts at:   1678    2294    4761    1678 
   Size:        616    2467    2531

  Fragments arranged by size: 

                2531    2467     616
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2075    2107    2350    2382    2625 
   Size:         32     135      32     503      32     243      32     243

Cuts at:   2625    2657    2816    2848    4255    4287    4329    4361    1373 
   Size:         32     159      32    1407      32      42      32    2626

  Fragments arranged by size: 

                2626    1407     503     243     243     159     135      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2958    3204     669 
   Size:        988     281    1020     246    3079

  Fragments arranged by size: 

                3079    1020     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2379     748 
   Size:       1631    3983
 
HpaI GTT'AAC

Cuts at:   3204    3204 
   Size:       5614
 
KpnI G_GTAC'C

Cuts at:   2939    2939 
   Size:       5614
 
MfeI C'AATT_G

Cuts at:   3213    3213 
   Size:       5614
 
MluI A'CGCG_T

Cuts at:   5490    5490 
   Size:       5614
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4728    4912    1305 
   Size:       3423     184    2007

  Fragments arranged by size: 

                3423    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3792

  Fragments arranged by size: 

                3792    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2400    2795    2850    3572    3931    4090     518 
   Size:        648    1234     395      55     722     359     159    2042

  Fragments arranged by size: 

                2042    1234     722     648     395     359     159      55
            
 
MspA1I CmG'CkG

Cuts at:   1606    3030    3707    4855    5100    1606 
   Size:       1424     677    1148     245    2120

  Fragments arranged by size: 

                2120    1424    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2434     513 
   Size:        552     939     430    3693

  Fragments arranged by size: 

                3693     939     552     430
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5614
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5614
 
NotI GC'GGCC_GC

Cuts at:   3061    3061 
   Size:       5614
 
NsiI A_TGCA'T

Cuts at:   2216    2216 
   Size:       5614
 
NspI r_CATG'y

Cuts at:   1132    1229    2972    4517    1132 
   Size:         97    1743    1545    2229

  Fragments arranged by size: 

                2229    1743    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5614
 
PciI A'CATG_T

Cuts at:   4513    4513 
   Size:       5614
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5614
 
PfoI T'CCnGG_A

Cuts at:   2748    2748 
   Size:       5614
 
PmeI GTTT'AAAC

Cuts at:   2918    2918 
   Size:       5614
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3683    3715    5196    5228    3683 
   Size:         32    1481      32    4069

  Fragments arranged by size: 

                4069    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5614
 
PsiI TTA'TAA

Cuts at:   3184    3184 
   Size:       5614
 
PspOMI G'GGCC_C

Cuts at:   2603    2603 
   Size:       5614
 
PstI C_TGCA'G

Cuts at:    830    2966     830 
   Size:       2136    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5614
 
SalI G'TCGA_C

Cuts at:   1936    2956    1936 
   Size:       1020    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2025    2300    2025 
   Size:        275    5339
 
SbfI CC_TGCA'GG

Cuts at:   2966    2966 
   Size:       5614
 
ScaI AGT'ACT

Cuts at:   3862    5574    3862 
   Size:       1712    3902
 
SfcI C'TryA_G

Cuts at:    826    1045    2962    4097    4778    4969     826 
   Size:        219    1917    1135     681     191    1471

  Fragments arranged by size: 

                1917    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5614
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5614
 
SmaI CCC'GGG

Cuts at:   1872    2159    2941    1872 
   Size:        287     782    4545

  Fragments arranged by size: 

                4545     782     287
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2428    3718    4619    4881     820 
   Size:        197     926     485    1290     901     262    1553

  Fragments arranged by size: 

                1553    1290     926     901     485     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3405     493 
   Size:       2912    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5614
 
SphI G_CATG'C

Cuts at:   1132    2972    1132 
   Size:       1840    3774
 
SspI AAT'ATT

Cuts at:      5      52    3538       5 
   Size:         47    3486    2081

  Fragments arranged by size: 

                3486    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5614
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2434    2892    3053     513 
   Size:        552     939     430     458     161    3074

  Fragments arranged by size: 

                3074     939     552     458     430     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2178    2585    3641    3658    3811    3996    4441 
   Size:       1255     598     407    1056      17     153     185     445

Cuts at:   4441     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1056     598     445     407     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2181    3860    5572 
   Size:        274      80      33      51    1443     204    1679    1712

Cuts at:   5572      96 
   Size:        138

  Fragments arranged by size: 

                1712    1679    1443     274     204     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2452    2484    5574    5606    2452 
   Size:         32    3090      32    2460

  Fragments arranged by size: 

                3090    2460      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5614
 
XbaI T'CTAG_A

Cuts at:   2950    2950 
   Size:       5614
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5614
 
XmaI C'CCGG_G

Cuts at:   1870    2157    2939    1870 
   Size:        287     782    4545

  Fragments arranged by size: 

                4545     782     287
 
XmnI GAAnn'nnTTC

Cuts at:   3743    3743 
   Size:       5614
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3422     276 
   Size:         53      83     186     936    1888    2468

  Fragments arranged by size: 

                2468    1888     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
     PstI     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     PacI     PmlI    PpuMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com