(Circular) (Six-base) MAPSORT of: pFN21AB8852.seq Check: 5394 from: 1 to: 4930 pFN21AB8852 4930 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2740 278 Size: 53 83 186 936 1204 2468 Fragments arranged by size: 2468 1204 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2273 1937 Size: 336 4594 Acc65I G'GTAC_C Cuts at: 2251 2251 Size: 4930 AclI AA'CG_TT Cuts at: 1563 3057 3430 1563 Size: 1494 373 3063 Fragments arranged by size: 3063 1494 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 4377 4645 1632 Size: 1361 1384 268 1917 Fragments arranged by size: 1917 1384 1361 268 AflII C'TTAA_G Cuts at: 820 1017 2072 820 Size: 197 1055 3678 Fragments arranged by size: 3678 1055 197 AflIII A'CryG_T Cuts at: 1203 3829 4806 1203 Size: 2626 977 1327 Fragments arranged by size: 2626 1327 977 AgeI A'CCGG_T Cuts at: 3761 3761 Size: 4930 AhdI GACnn_n'nnGTC Cuts at: 3659 3659 Size: 4930 AleI CACnn'nnGTG Cuts at: 1166 2151 2174 1166 Size: 985 23 3922 Fragments arranged by size: 3922 985 23 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4930 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 2195 Size: 32 4898 AlwNI CAG_nnn'CTG Cuts at: 2673 4245 2673 Size: 1572 3358 ApaLI G'TGCA_C Cuts at: 2987 4143 2987 Size: 1156 3774 ApoI r'AATT_y Cuts at: 1493 2239 2465 2617 4726 1493 Size: 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 3484 160 Size: 872 2452 1606 Fragments arranged by size: 2452 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4930 AvaI C'yCGr_G Cuts at: 1870 1943 2255 1870 Size: 73 312 4545 Fragments arranged by size: 4545 312 73 BamHI G'GATC_C Cuts at: 2158 2260 2627 2158 Size: 102 367 4461 Fragments arranged by size: 4461 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 3706 618 Size: 325 217 627 464 1455 1842 Fragments arranged by size: 1842 1455 627 464 325 217 BanII G_rGCy'C Cuts at: 1391 2249 1391 Size: 858 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3121 3155 1903 Size: 34 1184 34 3678 Fragments arranged by size: 3678 1184 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2822 4032 2822 Size: 1210 3720 BclI T'GATC_A Cuts at: 1548 1548 Size: 4930 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3541 136 Size: 107 122 71 631 605 1869 1525 Fragments arranged by size: 1869 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4925 4925 Size: 4930 BlpI GC'TnA_GC Cuts at: 2347 2347 Size: 4930 Bme1580I G_kGCm'C Cuts at: 1790 2181 2991 4147 1790 Size: 391 810 1156 2573 Fragments arranged by size: 2573 1156 810 391 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4930 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 3619 442 Size: 416 53 83 399 24 1244 958 1753 Fragments arranged by size: 1753 1244 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4930 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3590 1239 Size: 2351 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3055 3920 4218 3055 Size: 865 298 3767 Fragments arranged by size: 3767 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2009 3593 882 Size: 617 510 1584 2219 Fragments arranged by size: 2219 1584 617 510 BsaAI yAC'GTr Cuts at: 493 1204 2721 493 Size: 711 1517 2702 Fragments arranged by size: 2702 1517 711 BsaBI GATnn'nnATC Cuts at: 4924 4924 Size: 4930 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2737 3119 3754 275 Size: 53 83 186 936 1204 382 635 1451 Fragments arranged by size: 1451 1204 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3363 3761 4035 4182 4593 3363 Size: 398 274 147 411 3700 Fragments arranged by size: 3700 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 1178 Size: 30 165 30 792 30 3883 Fragments arranged by size: 3883 792 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 1186 Size: 1030 3900 BseYI C'CCAG_C Cuts at: 4133 4629 4133 Size: 496 4434 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2380 3141 4169 1540 Size: 275 186 379 761 1028 2301 Fragments arranged by size: 2301 1028 761 379 275 186 BsiHKAI G_wGCw'C Cuts at: 2224 2991 3076 4147 2224 Size: 767 85 1071 3007 Fragments arranged by size: 3007 1071 767 85 BsmI GAATG_Cn' Cuts at: 2441 2534 2441 Size: 93 4837 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2224 2249 2991 3076 4147 1391 Size: 399 391 43 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 85 43 25 BspEI T'CCGG_A Cuts at: 4593 4593 Size: 4930 BspHI T'CATG_A Cuts at: 2819 4549 2819 Size: 1730 3200 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 844 Size: 1441 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2817 1394 Size: 429 994 3507 Fragments arranged by size: 3507 994 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3425 3599 65 Size: 1183 2177 174 1396 Fragments arranged by size: 2177 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3574 3761 1700 Size: 110 1764 187 2869 Fragments arranged by size: 2869 1764 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4930 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4930 BssSI C'ACGA_G Cuts at: 2990 4002 2990 Size: 1012 3918 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4930 BstBI TT'CG_AA Cuts at: 3734 3734 Size: 4930 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4930 BstYI r'GATC_y Cuts at: 1972 2158 2260 2627 3012 3029 4481 4567 4579 Size: 186 102 367 385 17 1452 86 12 Cuts at: 4579 4925 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 186 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4191 Fragments arranged by size: 4191 683 56 BtsI GCAGTG_nn' Cuts at: 2446 3240 3260 2446 Size: 794 20 4116 Fragments arranged by size: 4116 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4930 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4895 DraI TTT'AAA Cuts at: 2234 2234 Size: 4930 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3937 809 Size: 507 2621 1802 Fragments arranged by size: 2621 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2377 3266 4895 Size: 54 1425 50 275 18 547 889 1629 Cuts at: 4895 8 Size: 43 Fragments arranged by size: 1629 1425 889 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2377 1537 Size: 275 565 4090 Fragments arranged by size: 4090 565 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2860 1353 Size: 1507 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3517 3891 4037 387 Size: 1064 138 1928 374 146 1280 Fragments arranged by size: 1928 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2993 3590 4377 4645 1239 Size: 393 1361 597 787 268 1524 Fragments arranged by size: 1524 1361 787 597 393 268 EcoRI G'AATT_C Cuts at: 2239 2617 2239 Size: 378 4552 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 4930 FspI TGC'GCA Cuts at: 3436 3436 Size: 4930 HaeII r_GCGC'y Cuts at: 1678 4077 1678 Size: 2399 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3571 3603 3645 3677 1373 Size: 32 135 32 1999 32 42 32 2626 Fragments arranged by size: 2626 1999 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2274 2520 669 Size: 988 281 336 246 3079 Fragments arranged by size: 3079 988 336 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4930 HpaI GTT'AAC Cuts at: 2520 2520 Size: 4930 KpnI G_GTAC'C Cuts at: 2255 2255 Size: 4930 MfeI C'AATT_G Cuts at: 2529 2529 Size: 4930 MluI A'CGCG_T Cuts at: 4806 4806 Size: 4930 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2075 4044 4228 1305 Size: 770 1969 184 2007 Fragments arranged by size: 2007 1969 770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3108 Fragments arranged by size: 3108 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2174 2888 3247 3406 518 Size: 648 985 23 714 359 159 2042 Fragments arranged by size: 2042 985 714 648 359 159 23 MspA1I CmG'CkG Cuts at: 1606 2346 3023 4171 4416 1606 Size: 740 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4378 NdeI CA'TA_TG Cuts at: 387 387 Size: 4930 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4930 NotI GC'GGCC_GC Cuts at: 2377 2377 Size: 4930 NspI r_CATG'y Cuts at: 1132 1229 2288 3833 1132 Size: 97 1059 1545 2229 Fragments arranged by size: 2229 1545 1059 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4930 PciI A'CATG_T Cuts at: 3829 3829 Size: 4930 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4930 PfoI T'CCnGG_A Cuts at: 2199 2199 Size: 4930 PmeI GTTT'AAAC Cuts at: 2234 2234 Size: 4930 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2195 2227 2999 3031 4512 4544 2195 Size: 32 772 32 1481 32 2581 Fragments arranged by size: 2581 1481 772 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4930 PsiI TTA'TAA Cuts at: 2500 2500 Size: 4930 PstI C_TGCA'G Cuts at: 830 2282 830 Size: 1452 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4930 SalI G'TCGA_C Cuts at: 1936 2272 1936 Size: 336 4594 SbfI CC_TGCA'GG Cuts at: 2282 2282 Size: 4930 ScaI AGT'ACT Cuts at: 3178 4890 3178 Size: 1712 3218 SfcI C'TryA_G Cuts at: 826 1045 2278 3413 4094 4285 826 Size: 219 1233 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4930 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4930 SmaI CCC'GGG Cuts at: 1872 2257 1872 Size: 385 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 3034 3935 4197 820 Size: 197 926 129 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 2721 493 Size: 2228 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4930 SphI G_CATG'C Cuts at: 1132 2288 1132 Size: 1156 3774 SspI AAT'ATT Cuts at: 5 52 2854 5 Size: 47 2802 2081 Fragments arranged by size: 2802 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4930 StyI C'CwwG_G Cuts at: 513 1065 2109 2369 513 Size: 552 1044 260 3074 Fragments arranged by size: 3074 1044 552 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2957 2974 3127 3312 3757 325 Size: 1255 1377 17 153 185 445 1498 Fragments arranged by size: 1498 1377 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3176 4888 96 Size: 274 80 33 51 1443 1199 1712 138 Fragments arranged by size: 1712 1443 1199 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4890 4922 4890 Size: 32 4898 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4930 XbaI T'CTAG_A Cuts at: 2266 2266 Size: 4930 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4930 XmaI C'CCGG_G Cuts at: 1870 2255 1870 Size: 385 4545 XmnI GAAnn'nnTTC Cuts at: 2195 2210 2213 3059 2195 Size: 15 3 846 4066 Fragments arranged by size: 4066 846 15 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2738 276 Size: 53 83 186 936 1204 2468 Fragments arranged by size: 2468 1204 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI