################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BmyI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 676 GTCTAC GT^[AC][GT]AC AfaI 561 GTAC GT^AC AflIII 536 ACATGT A^C[GA][CT]GT AvaI 123 CTCGGG C^[CT]CG[GA]G BalI 519 TGGCCA TGG^CCA BamHI 707 GGATCC G^GATCC BbsI 208 GTCTTC GAAGAC(2/6) BcgI 222 GCATAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 270 CCGGG CC^[GC]GG BlpI 392 GCTCAGC GC^T[ACGT]AGC BpmI 379 CTGGAG CTGGAG(16/14) Bpu1102I 392 GCTCAGC GC^T[ACGT]AGC BpuAI 208 GTCTTC GAAGAC(2/6) BsaI 611 GAGACC GGTCTC(1/5) BsmAI 611 GAGAC GTCTC(1/5) BsoBI 123 CTCGGG C^[CT]CG[GA]G BspLU11I 536 ACATGT A^CATGT BstYI 707 GGATCC [GA]^GATC[CT] CelII 392 GCTCAGC GC^T[ACGT]AGC CfoI 330 GCGC GCG^C DraIII 481 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 519 TGGCCA [CT]^GGCC[GA] HapII 270 CCGG C^CGG HhaI 330 GCGC GCG^C HinP1I 330 GCGC G^CGC HpaII 270 CCGG C^CGG MaeII 686 ACGT A^CGT MaeIII 641 GTAAC ^GT[ACGT]AC MflI 707 GGATCC [GA]^GATC[CT] MluNI 519 TGGCCA TGG^CCA MscI 519 TGGCCA TGG^CCA MspI 270 CCGG C^CGG NciI 270 CCGGG CC^[GC]GG PflMI 414 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 561 GTAC GT^AC SexAI 482 ACCTGGT A^CC[AT]GGT SfaNI 295 GATGC GCATC(5/9) SphI 507 GCATGC GCATG^C StuI 624 AGGCCT AGG^CCT Van91I 414 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 707 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BseRI 49 GAGGAG GAGGAG(10/8) 496 GAGGAG BsmFI 386 GGGAC GGGAC(10/14) 489 GTCCC BsrI 704 ACTGG ACTGG(1/-1) 465 CCAGT DdeI 22 CTGAG C^T[ACGT]AG 393 CTCAG DraII 146 GGGTCCT [GA]G^G[ACGT]CC[CT] 167 GGGTCCT EcoO109I 146 GGGTCCT [GA]G^G[ACGT]CC[CT] 167 GGGTCCT NspI 507 GCATGC [GA]CATG^[CT] 536 ACATGT PleI 24 GAGTC GAGTC(4/5) 674 GAGTC PpuMI 146 GGGTCCT [GA]G^G[AT]CC[CT] 167 GGGTCCT PstI 35 CTGCAG CTGCA^G 690 CTGCAG PvuII 176 CAGCTG CAG^CTG 408 CAGCTG SfcI 35 CTGCAG C^T[GA][CT]AG 690 CTGCAG XcmI 129 CCACTTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 579 CCAGGGTGATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI TspRI