(Linear) (Six-base) MAPSORT of: KIEE3654.seq Check: 4040 from: 1 to: 732 KIEE3654.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AccI GT'mk_AC Cuts at: 0 677 732 Size: 677 55 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 732 Size: 187 545 AflIII A'CryG_T Cuts at: 0 536 732 Size: 536 196 AvaI C'yCGr_G Cuts at: 0 123 732 Size: 123 609 BamHI G'GATC_C Cuts at: 0 707 732 Size: 707 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 201 732 Size: 201 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 211 245 732 Size: 211 34 487 Fragments arranged by size: 487 211 34 BlpI GC'TnA_GC Cuts at: 0 393 732 Size: 393 339 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 634 732 Size: 634 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 732 Size: 400 332 BsaI GGTCTCn'nnnn_ Cuts at: 0 605 732 Size: 605 127 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 64 511 732 Size: 64 447 221 Fragments arranged by size: 447 221 64 BseYI C'CCAG_C Cuts at: 0 342 425 547 732 Size: 342 83 122 185 Fragments arranged by size: 342 185 122 83 BstYI r'GATC_y Cuts at: 0 707 732 Size: 707 25 BtsI GCAGTG_nn' Cuts at: 0 183 732 Size: 183 549 DraIII CAC_nnn'GTG Cuts at: 0 486 732 Size: 486 246 EaeI y'GGCC_r Cuts at: 0 519 732 Size: 519 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 89 732 Size: 89 643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 400 732 Size: 187 213 332 Fragments arranged by size: 332 213 187 EcoO109I rG'GnC_Cy Cuts at: 0 147 168 732 Size: 147 21 564 Fragments arranged by size: 564 147 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 76 108 732 Size: 76 32 624 Fragments arranged by size: 624 76 32 MscI TGG'CCA Cuts at: 0 521 732 Size: 521 211 MspA1I CmG'CkG Cuts at: 0 178 410 600 732 Size: 178 232 190 132 Fragments arranged by size: 232 190 178 132 NspI r_CATG'y Cuts at: 0 511 540 732 Size: 511 29 192 Fragments arranged by size: 511 192 29 PciI A'CATG_T Cuts at: 0 536 732 Size: 536 196 PflMI CCAn_nnn'nTGG Cuts at: 0 420 732 Size: 420 312 PpuMI rG'GwC_Cy Cuts at: 0 147 168 732 Size: 147 21 564 Fragments arranged by size: 564 147 21 PstI C_TGCA'G Cuts at: 0 39 694 732 Size: 39 655 38 Fragments arranged by size: 655 39 38 PvuII CAG'CTG Cuts at: 0 178 410 732 Size: 178 232 322 Fragments arranged by size: 322 232 178 SexAI A'CCwGG_T Cuts at: 0 482 732 Size: 482 250 SfcI C'TryA_G Cuts at: 0 35 690 732 Size: 35 655 42 Fragments arranged by size: 655 42 35 SphI G_CATG'C Cuts at: 0 511 732 Size: 511 221 StuI AGG'CCT Cuts at: 0 626 732 Size: 626 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 544 732 Size: 544 188 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 152 184 472 504 732 Size: 152 32 288 32 228 Fragments arranged by size: 288 228 152 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 136 586 732 Size: 136 450 146 Fragments arranged by size: 450 146 136 Enzymes that do cut: AccI AcuI AflIII AvaI BamHI BbsI BcgI BlpI BplI BpmI BsaI BseRI BseYI BstYI BtsI DraIII EaeI EciI Eco57MI EcoO109I Hin4I MscI MspA1I NspI PciI PflMI PpuMI PstI PvuII SexAI SfcI SphI StuI TaqII TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI