Restriction map for
pFN21AE1316
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DpnII DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI MaeII MboI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeII NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 51 CGCG CG^CG
AflIII 764 ACATGT A^C[GA][CT]GT
AlwNI 281 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 439 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 792 TGGCCA TGG^CCA
BfaI 754 CTAG C^TAG
BmyI 101 GGGCAC G[AGT]GC[ACT]^C
BpmI 167 CTGGAG CTGGAG(16/14)
BsaBI 340 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 566 GGTCTC GGTCTC(1/5)
BseRI 215 GAGGAG GAGGAG(10/8)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiYI 65 CCTCGGTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 65 CCTCGGTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 712 GAGACG CGTCTC(1/5)
Bsp1286I 101 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 764 ACATGT A^CATGT
BsrBI 85 GAGCGG CCGCTC(-3/-3)
BsrI 166 ACTGG ACTGG(1/-1)
BstUI 51 CGCG CG^CG
CfoI 114 GCGC GCG^C
DraII 282 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 50 CCGCGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoO109I 282 AGGACCT [GA]G^G[ACGT]CC[CT]
HhaI 114 GCGC GCG^C
HinP1I 114 GCGC G^CGC
HindIII 409 AAGCTT A^AGCTT
HphI 526 GGTGA GGTGA(8/7)
KspI 50 CCGCGG CCGC^GG
MaeI 754 CTAG C^TAG
MaeIII 549 GTTAC ^GT[ACGT]AC
MamI 340 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 792 TGGCCA TGG^CCA
MscI 792 TGGCCA TGG^CCA
MvnI 51 CGCG CG^CG
NdeI 806 CATATG CA^TATG
NspI 764 ACATGT [GA]CATG^[CT]
PpuMI 282 AGGACCT [GA]G^G[AT]CC[CT]
PstI 384 CTGCAG CTGCA^G
SacII 50 CCGCGG CCGC^GG
SfcI 384 CTGCAG C^T[GA][CT]AG
TaqI 83 TCGA T^CGA
TspRI 104 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 83 TCGA T^CGA
XmnI 439 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 768 GTAC GT^AC
782 GTAC
AluI 56 AGCT AG^CT
410 AGCT
AvaII 283 GGACC G^G[AT]CC
739 GGACC
BcnI 122 CCCGG CC^[GC]GG
134 CCCGG
BglI 10 GCCGACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
121 GCCCGGCTGGC
BsaJI 50 CCGCGG C^C[ACGT][ACGT]GG
65 CCTCGG
BsmAI 712 GAGAC GTCTC(1/5)
567 GTCTC
BstNI 117 CCAGG CC^[AT]GG
280 CCAGG
EaeI 8 CGGCCG [CT]^GGCC[GA]
792 TGGCCA
EarI 232 GAAGAG CTCTTC(1/4)
561 GAAGAG
EcoRII 117 CCAGG ^CC[AT]GG
280 CCAGG
HinfI 340 GATTC G^A[ACGT]TC
504 GAATC
Ksp632I 232 GAAGAG CTCTTC(1/4)
561 GAAGAG
MseI 485 TTAA T^TAA
544 TTAA
MslI 724 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
730 CATGAGTATG
MspA1I 50 CCGCGG C[AC]G^C[GT]G
97 CAGCGG
MvaI 117 CCAGG CC^[AT]GG
280 CCAGG
NciI 122 CCCGG CC^[GC]GG
134 CCCGG
RsaI 768 GTAC GT^AC
782 GTAC
SfaNI 108 GATGC GCATC(5/9)
391 GATGC
TfiI 340 GATTC G^A[AT]TC
504 GAATC
Tru9I 485 TTAA T^TAA
544 TTAA
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AcsI ApoI BbvI BssKI
Cac8I Cfr13I FauI Fnu4HI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MwoI NlaIII Sau96I ScrFI
TseI Tsp509I