Restriction map for pFN21AE1316
 (Circular) (Six-base) MAPSORT of: pFN21AE1316.seq  Check: 9323  from: 1  to: 5524



pFN21AE1316  5524 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:45  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3334     278 
   Size:         53      83     186     936    1798    2468

  Fragments arranged by size: 

                2468    1798     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2867    1937 
   Size:        930    4594
 
Acc65I G'GTAC_C

Cuts at:   2845    2845 
   Size:       5524
 
AclI AA'CG_TT

Cuts at:   1563    3651    4024    1563 
   Size:       2088     373    3063

  Fragments arranged by size: 

                3063    2088     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3587    4971    5239    1632 
   Size:       1955    1384     268    1917

  Fragments arranged by size: 

                1955    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5327
 
AflIII A'CryG_T

Cuts at:   1203    2769    4423    5400    1203 
   Size:       1566    1654     977    1327

  Fragments arranged by size: 

                1654    1566    1327     977
 
AgeI A'CCGG_T

Cuts at:   4355    4355 
   Size:       5524
 
AhdI GACnn_n'nnGTC

Cuts at:   4253    4253 
   Size:       5524
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5524
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5524
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2259    2291    2259 
   Size:         32    5492
 
AlwNI CAG_nnn'CTG

Cuts at:   2291    3267    4839    2291 
   Size:        976    1572    2976

  Fragments arranged by size: 

                2976    1572     976
 
ApaLI G'TGCA_C

Cuts at:   3581    4737    3581 
   Size:       1156    4368
 
ApoI r'AATT_y

Cuts at:   1493    2300    2336    2492    2657    2833    3059    3211    5320 
   Size:        807      36     156     165     176     226     152    2109

Cuts at:   5320    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     807     226     176     165     156     152
                  36
 
AseI AT'TA_AT

Cuts at:    160    1032    4078     160 
   Size:        872    3046    1606

  Fragments arranged by size: 

                3046    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5524
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2849    1870 
   Size:         73     906    4545

  Fragments arranged by size: 

                4545     906      73
 
BamHI G'GATC_C

Cuts at:   2854    3221    2854 
   Size:        367    5157
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2845    4300     618 
   Size:        325     217     627    1058    1455    1842

  Fragments arranged by size: 

                1842    1455    1058     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2843    1391 
   Size:       1452    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4553
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3715    3749    1903 
   Size:         34    1778      34    3678

  Fragments arranged by size: 

                3678    1778      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3416    4626    3416 
   Size:       1210    4314
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5524
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2021    2132 
   Size:        107     122      71     631     605     337      12     111

Cuts at:   2132    4135     136 
   Size:       2003    1525

  Fragments arranged by size: 

                2003    1525     631     605     337     122     111     107
                  71      12
 
BglII A'GATC_T

Cuts at:   5519    5519 
   Size:       5524
 
BlpI GC'TnA_GC

Cuts at:   2941    2941 
   Size:       5524
 
Bme1580I G_kGCm'C

Cuts at:   1790    2110    3585    4741    1790 
   Size:        320    1475    1156    2573

  Fragments arranged by size: 

                2573    1475    1156     320
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5524
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3255    4213     442 
   Size:        416      53      83     399      24    1838     958    1753

  Fragments arranged by size: 

                1838    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5524
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2193    4184    1239 
   Size:        954    1991    2579

  Fragments arranged by size: 

                2579    1991     954
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3649    4514    4812    3649 
   Size:        865     298    4361

  Fragments arranged by size: 

                4361     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2577    4187     882 
   Size:        617    1078    1610    2219

  Fragments arranged by size: 

                2219    1610    1078     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3315     493 
   Size:        711    2111    2702

  Fragments arranged by size: 

                2702    2111     711
 
BsaBI GATnn'nnATC

Cuts at:   2349    5518    2349 
   Size:       3169    2355
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3331    3713    4348     275 
   Size:         53      83     186     936    1798     382     635    1451

  Fragments arranged by size: 

                1798    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3957    4355    4629    4776    5187    3957 
   Size:        398     274     147     411    4294

  Fragments arranged by size: 

                4294     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5299

  Fragments arranged by size: 

                5299     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2235    1186 
   Size:       1049    4475
 
BseYI C'CCAG_C

Cuts at:   4727    5223    4727 
   Size:        496    5028
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4951
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2016    2974    3735    4763    1540 
   Size:        275     186      15     958     761    1028    2301

  Fragments arranged by size: 

                2301    1028     958     761     275     186      15
 
BsiHKAI G_wGCw'C

Cuts at:   3585    3670    4741    3585 
   Size:         85    1071    4368

  Fragments arranged by size: 

                4368    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3035    3128    3035 
   Size:         93    5431
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2711    2711 
   Size:       5524
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2110    2843    3585    3670    4741    1391 
   Size:        399     320     733     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     733     399     320      85
 
BspEI T'CCGG_A

Cuts at:   5187    5187 
   Size:       5524
 
BspHI T'CATG_A

Cuts at:   3413    5143    3413 
   Size:       1730    3794
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2879     844 
   Size:       2035    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2092    3411    1394 
   Size:        429     269    1319    3507

  Fragments arranged by size: 

                3507    1319     429     269
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4019    4193      65 
   Size:       1183    2771     174    1396

  Fragments arranged by size: 

                2771    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4168    4355    1700 
   Size:        110    2358     187    2869

  Fragments arranged by size: 

                2869    2358     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5524
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5524
 
BssSI C'ACGA_G

Cuts at:   3584    4596    3584 
   Size:       1012    4512
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5524
 
BstBI TT'CG_AA

Cuts at:   4328    4328 
   Size:       5524
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5524
 
BstYI r'GATC_y

Cuts at:   1972    2854    3221    3606    3623    5075    5161    5173    5519 
   Size:        882     367     385      17    1452      86      12     346

Cuts at:   5519    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     882     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2055     513 
   Size:        552     939      51    3982

  Fragments arranged by size: 

                3982     939     552      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4785

  Fragments arranged by size: 

                4785     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3040    3834    3854    3040 
   Size:        794      20    4710

  Fragments arranged by size: 

                4710     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5524
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5489
 
DraI TTT'AAA

Cuts at:   2828    2828 
   Size:       5524
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4531     809 
   Size:        507    3215    1802

  Fragments arranged by size: 

                3215    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2013    2797    2971 
   Size:         54    1425      50     275      18     183     784     174

Cuts at:   2971    3860    5489       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     784     275     183     174      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2013    2971    1537 
   Size:        275     201     958    4090

  Fragments arranged by size: 

                4090     958     275     201
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2232    2561    3454    1353 
   Size:        879     329     893    3423

  Fragments arranged by size: 

                3423     893     879     329
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4111    4485    4631     387 
   Size:       1064     138    2522     374     146    1280

  Fragments arranged by size: 

                2522    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2193    3587    4184    4971    5239    1239 
   Size:        393     561    1394     597     787     268    1524

  Fragments arranged by size: 

                1524    1394     787     597     561     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2288    2288 
   Size:       5524
 
EcoRI G'AATT_C

Cuts at:   2833    3211    2833 
   Size:        378    5146
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2465    2465 
   Size:       5524
 
FspI TGC'GCA

Cuts at:   4030    4030 
   Size:       5524
 
HaeII r_GCGC'y

Cuts at:   1678    4671    1678 
   Size:       2993    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2713    2745    4165    4197    4239 
   Size:         32     135      32    1141      32    1420      32      42

Cuts at:   4239    4271    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1420    1141     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2868    3114     669 
   Size:        988     281     930     246    3079

  Fragments arranged by size: 

                3079     988     930     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2414     748 
   Size:       1666    3858
 
HpaI GTT'AAC

Cuts at:   3114    3114 
   Size:       5524
 
KpnI G_GTAC'C

Cuts at:   2849    2849 
   Size:       5524
 
MfeI C'AATT_G

Cuts at:   3123    3123 
   Size:       5524
 
MluI A'CGCG_T

Cuts at:   5400    5400 
   Size:       5524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2647    4638    4822    1305 
   Size:       1342    1991     184    2007

  Fragments arranged by size: 

                2007    1991    1342     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2799      10 
   Size:         54    1425     343     967    2735

  Fragments arranged by size: 

                2735    1425     967     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2733    2739    3482    3841    4000     518 
   Size:        648    1567       6     743     359     159    2042

  Fragments arranged by size: 

                2042    1567     743     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2057    2104    2940    3617    4765    5010    1606 
   Size:        451      47     836     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     836     677     451     245      47
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4033

  Fragments arranged by size: 

                4033     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2812     387 
   Size:       2425    3099
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5524
 
NotI GC'GGCC_GC

Cuts at:   2971    2971 
   Size:       5524
 
NspI r_CATG'y

Cuts at:   1132    1229    2773    2882    4427    1132 
   Size:         97    1544     109    1545    2229

  Fragments arranged by size: 

                2229    1545    1544     109      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5524
 
PciI A'CATG_T

Cuts at:   2769    4423    2769 
   Size:       1654    3870
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5524
 
PmeI GTTT'AAAC

Cuts at:   2828    2828 
   Size:       5524
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3593    3625    5106    5138    3593 
   Size:         32    1481      32    3979

  Fragments arranged by size: 

                3979    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2288    2288 
   Size:       5524
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5524
 
PsiI TTA'TAA

Cuts at:   2420    3094    2420 
   Size:        674    4850
 
PstI C_TGCA'G

Cuts at:    830    2393    2876     830 
   Size:       1563     483    3478

  Fragments arranged by size: 

                3478    1563     483
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5524
 
SacII CC_GC'GG

Cuts at:   2058    2058 
   Size:       5524
 
SalI G'TCGA_C

Cuts at:   1936    2866    1936 
   Size:        930    4594
 
SbfI CC_TGCA'GG

Cuts at:   2876    2876 
   Size:       5524
 
ScaI AGT'ACT

Cuts at:   3772    5484    3772 
   Size:       1712    3812
 
SfcI C'TryA_G

Cuts at:    826    1045    2389    2872    4007    4688    4879     826 
   Size:        219    1344     483    1135     681     191    1471

  Fragments arranged by size: 

                1471    1344    1135     681     483     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5524
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5524
 
SmaI CCC'GGG

Cuts at:   1872    2851    1872 
   Size:        979    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3628    4529    4791     820 
   Size:        197     926    1685     901     262    1553

  Fragments arranged by size: 

                1685    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3315     493 
   Size:       2822    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5524
 
SphI G_CATG'C

Cuts at:   1132    2882    1132 
   Size:       1750    3774
 
SspI AAT'ATT

Cuts at:      5      52    3448       5 
   Size:         47    3396    2081

  Fragments arranged by size: 

                3396    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5524
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2963     513 
   Size:        552     939     959    3074

  Fragments arranged by size: 

                3074     959     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3551    3568    3721    3906    4351     325 
   Size:       1255    1971      17     153     185     445    1498

  Fragments arranged by size: 

                1971    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2786    3770    5482 
   Size:        274      80      33      51    1443     809     984    1712

Cuts at:   5482      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443     984     809     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5484    5516    5484 
   Size:         32    5492
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5524
 
XbaI T'CTAG_A

Cuts at:   2860    2860 
   Size:       5524
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5524
 
XmaI C'CCGG_G

Cuts at:   1870    2849    1870 
   Size:        979    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2448    3653    2448 
   Size:       1205    4319
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3332     276 
   Size:         53      83     186     936    1798    2468

  Fragments arranged by size: 

                2468    1798     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    SacII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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