################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI BalI BamHI BcgI BciVI BclI BfrI BglII BlpI BpmI Bpu1102I BsaBI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 590 GGTACC G^GTACC AcyI 208 GGCGCC G[GA]^CG[CT]C AfaI 591 GTAC GT^AC AlwI 162 GATCC GGATC(4/5) Aor51HI 83 AGCGCT AGC^GCT Asp718I 590 GGTACC G^GTACC AspI 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 313 CCCGAG C^[CT]CG[GA]G AviII 878 TGCGCA TGC^GCA AvrII 117 CCTAGG C^CTAGG BbeI 208 GGCGCC GGCGC^C BbrPI 148 CACGTG CAC^GTG BbsI 550 GAAGAC GAAGAC(2/6) BbvCI 301 GCTGAGG CCTCAGC(-5/-2) BfaI 118 CTAG C^TAG BlnI 117 CCTAGG C^CTAGG BpuAI 550 GAAGAC GAAGAC(2/6) BsaAI 148 CACGTG [CT]AC^GT[GA] BsaHI 208 GGCGCC G[GA]^CG[CT]C BsoBI 313 CCCGAG C^[CT]CG[GA]G BspMI 130 ACCTGC ACCTGC(4/8) BsrBI 96 CCGCTC CCGCTC(-3/-3) BstAPI 755 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 161 AGATCC [GA]^GATC[CT] DpnII 162 GATC ^GATC DraIII 490 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 83 AGCGCT AGC^GCT EcoT14I 117 CCTAGG C^C[AT][AT]GG FspI 878 TGCGCA TGC^GCA Hin1I 208 GGCGCC G[GA]^CG[CT]C HinfI 504 GACTC G^A[ACGT]TC KasI 208 GGCGCC G^GCGCC KpnI 590 GGTACC GGTAC^C MaeI 118 CTAG C^TAG MboI 162 GATC ^GATC MflI 161 AGATCC [GA]^GATC[CT] MslI 148 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 331 GCCGGC GCC^GGC NarI 208 GGCGCC GG^CGCC NdeII 162 GATC ^GATC NgoMIV 331 GCCGGC G^CCGGC NspI 429 GCATGC [GA]CATG^[CT] PflFI 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 513 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 504 GACTC GAGTC(4/5) PmaCI 148 CACGTG CAC^GTG PmlI 148 CACGTG CAC^GTG RsaI 591 GTAC GT^AC Sau3AI 162 GATC ^GATC SexAI 806 ACCTGGT A^CC[AT]GGT SfoI 208 GGCGCC GGC^GCC SmlI 691 CTTGAG C^T[CT][GA]AG SphI 429 GCATGC GCATG^C StuI 120 AGGCCT AGG^CCT StyI 117 CCTAGG C^C[AT][AT]GG Tth111I 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 513 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 161 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BglI 559 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 670 GCCCAGAGGGC BsaWI 464 TCCGGT [AT]^CCGG[AT] 546 ACCGGA BsgI 28 CTGCAC GTGCAG(16/14) 496 GTGCAG BsmAI 479 GAGAC GTCTC(1/5) 502 GAGAC BsrFI 61 GCCGGT [GA]^CCGG[CT] 331 GCCGGC BstF5I 153 GGATG GGATG(2/0) 711 GGATG BstXI 147 CCACGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 707 CCAGGGATGTGG Cfr10I 61 GCCGGT [GA]^CCGG[CT] 331 GCCGGC DsaI 33 CCGTGG C^C[GA][CT]GG 535 CCGTGG EaeI 289 TGGCCG [CT]^GGCC[GA] 801 CGGCCA EarI 196 GAAGAG CTCTTC(1/4) 476 GAAGAG FauI 95 CCCGC CCCGC(4/6) 407 GCGGG FokI 153 GGATG GGATG(9/13) 711 GGATG HaeII 83 AGCGCT [GA]GCGC^[CT] 208 GGCGCC HgaI 553 GACGC GACGC(5/10) 267 GCGTC HphI 64 GGTGA GGTGA(8/7) 363 GGTGA Ksp632I 196 GAAGAG CTCTTC(1/4) 476 GAAGAG MaeII 149 ACGT A^CGT 751 ACGT NlaIII 249 CATG CATG^ 430 CATG PpuMI 3 GGGACCT [GA]G^G[AT]CC[CT] 530 GGGACCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoNI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PstI PvuII Sau96I ScrFI SfcI TseI TspRI