(Circular) (Six-base) MAPSORT of: pFN21AE3618.seq Check: 8391 from: 1 to: 5599 pFN21AE3618 5599 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2144 2144 Size: 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2595 2920 2595 Size: 325 5274 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3662 5046 5314 1632 Size: 2030 1384 268 1917 Fragments arranged by size: 2030 1917 1384 268 AfeI AGC'GCT Cuts at: 2090 2090 Size: 5599 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 4498 5475 1203 Size: 3295 977 1327 Fragments arranged by size: 3295 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2897 1184 Size: 1713 3886 AlwNI CAG_nnn'CTG Cuts at: 2145 2170 2464 2524 2567 3342 4914 2145 Size: 25 294 60 43 775 1572 2830 Fragments arranged by size: 2830 1572 775 294 60 43 25 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2908 3134 3286 5395 1493 Size: 1415 226 152 2109 1697 Fragments arranged by size: 2109 1697 1415 226 152 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2924 1870 Size: 73 375 606 4545 Fragments arranged by size: 4545 606 375 73 AvrII C'CTAG_G Cuts at: 2122 2122 Size: 5599 BamHI G'GATC_C Cuts at: 2929 3296 2929 Size: 367 5232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2213 2311 2595 2920 4375 Size: 325 217 627 426 98 284 325 1455 Cuts at: 4375 618 Size: 1842 Fragments arranged by size: 1842 1455 627 426 325 325 284 217 98 BanII G_rGCy'C Cuts at: 1391 2261 2531 2703 2866 2918 1391 Size: 870 270 172 163 52 4072 Fragments arranged by size: 4072 870 270 172 163 52 BbeI G_GCGC'C Cuts at: 2217 2217 Size: 5599 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2562 928 Size: 971 663 3965 Fragments arranged by size: 3965 971 663 BbvCI CC'TCA_GC Cuts at: 2307 2307 Size: 5599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3491 4701 3491 Size: 1210 4389 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2570 2681 4210 Size: 107 122 71 631 605 898 111 1529 Cuts at: 4210 136 Size: 1525 Fragments arranged by size: 1529 1525 898 631 605 122 111 107 71 BglII A'GATC_T Cuts at: 5594 5594 Size: 5599 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 2644 2833 2852 3660 4816 1790 Size: 854 189 19 808 1156 2573 Fragments arranged by size: 2573 1156 854 808 189 19 BmgBI CAC'GTC Cuts at: 1318 2757 1318 Size: 1439 4160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3330 4288 Size: 416 53 83 399 24 651 1262 958 Cuts at: 4288 442 Size: 1753 Fragments arranged by size: 1753 1262 958 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4259 1239 Size: 3020 2579 Bpu10I CC'TnA_GC Cuts at: 2307 2601 2307 Size: 294 5305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 3724 4589 4887 2717 Size: 1007 865 298 3429 Fragments arranged by size: 3429 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2155 3390 493 Size: 711 951 1235 2702 Fragments arranged by size: 2702 1235 951 711 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 3406 3788 4423 Size: 53 83 186 936 681 1192 382 635 Cuts at: 4423 275 Size: 1451 Fragments arranged by size: 1451 1192 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2469 2551 4032 4430 4704 4851 5262 2469 Size: 82 1481 398 274 147 411 2806 Fragments arranged by size: 2806 1481 411 398 274 147 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2741 2771 1178 Size: 30 165 30 1338 30 4006 Fragments arranged by size: 4006 1338 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2219 2222 2279 2762 1186 Size: 1024 9 3 57 483 4023 Fragments arranged by size: 4023 1024 483 57 9 3 BseYI C'CCAG_C Cuts at: 2450 2517 2524 4802 5298 2450 Size: 67 7 2278 496 2751 Fragments arranged by size: 2751 2278 496 67 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 2522 1163 Size: 573 282 504 4240 Fragments arranged by size: 4240 573 504 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3810 4838 1540 Size: 275 186 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3660 3745 4816 3660 Size: 85 1071 4443 Fragments arranged by size: 4443 1071 85 BsmI GAATG_Cn' Cuts at: 3110 3203 3110 Size: 93 5506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2531 2644 2703 2833 2852 2866 Size: 399 471 270 113 59 130 19 14 Cuts at: 2866 2918 3660 3745 4816 1391 Size: 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 270 130 113 85 59 52 19 14 BspEI T'CCGG_A Cuts at: 5262 5262 Size: 5599 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2144 2954 844 Size: 1300 810 3489 Fragments arranged by size: 3489 1300 810 BsrBI CCG'CTC Cuts at: 1394 1823 2103 3486 1394 Size: 429 280 1383 3507 Fragments arranged by size: 3507 1383 429 280 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2336 4243 4430 1700 Size: 110 256 270 1907 187 2869 Fragments arranged by size: 2869 1907 270 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 3659 4671 3659 Size: 1012 4587 BstAPI GCAn_nnn'nTGC Cuts at: 794 2766 794 Size: 1972 3627 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5599 BstXI CCAn_nnnn'nTGG Cuts at: 2159 2719 2159 Size: 560 5039 BstYI r'GATC_y Cuts at: 1972 2166 2929 3296 3681 3698 5150 5236 5248 Size: 194 763 367 385 17 1452 86 12 Cuts at: 5248 5594 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 763 385 367 346 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2038 2540 513 Size: 552 939 34 502 3572 Fragments arranged by size: 3572 939 552 502 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4860 Fragments arranged by size: 4860 683 56 BtsI GCAGTG_nn' Cuts at: 3115 3909 3929 3115 Size: 794 20 4785 Fragments arranged by size: 4785 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DraIII CAC_nnn'GTG Cuts at: 2500 2500 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 809 Size: 507 3290 1802 Fragments arranged by size: 3290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2294 2806 3046 Size: 54 1425 50 275 18 464 512 240 Cuts at: 3046 3935 5564 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 512 464 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 1537 Size: 275 1234 4090 Fragments arranged by size: 4090 1234 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2196 2476 3529 1353 Size: 843 280 1053 3423 Fragments arranged by size: 3423 1053 843 280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4186 4560 4706 387 Size: 1064 138 2597 374 146 1280 Fragments arranged by size: 2597 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3662 4259 5046 5314 1239 Size: 393 2030 597 787 268 1524 Fragments arranged by size: 2030 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2121 2192 2230 2121 Size: 71 38 5490 Fragments arranged by size: 5490 71 38 EcoO109I rG'GnC_Cy Cuts at: 2009 2237 2392 2536 2009 Size: 228 155 144 5072 Fragments arranged by size: 5072 228 155 144 EcoRI G'AATT_C Cuts at: 2908 3286 2908 Size: 378 5221 FspI TGC'GCA Cuts at: 2885 4105 2885 Size: 1220 4379 FspAI rTGC'GCAy Cuts at: 2885 2885 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 2092 2217 4746 1678 Size: 414 125 2529 2531 Fragments arranged by size: 2531 2529 414 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2475 2507 2615 2647 2853 Size: 32 135 32 903 32 108 32 206 Cuts at: 2853 2885 4240 4272 4314 4346 1373 Size: 32 1355 32 42 32 2626 Fragments arranged by size: 2626 1355 903 206 135 108 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2943 3189 669 Size: 988 281 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KasI G'GCGC_C Cuts at: 2213 2213 Size: 5599 KpnI G_GTAC'C Cuts at: 2599 2924 2599 Size: 325 5274 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4713 4897 1305 Size: 3408 184 2007 Fragments arranged by size: 3408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3777 Fragments arranged by size: 3777 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 3557 3916 4075 518 Size: 648 991 1400 359 159 2042 Fragments arranged by size: 2042 1400 991 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 2299 2454 2521 2731 2845 3015 3692 Size: 573 120 155 67 210 114 170 677 Cuts at: 3692 4840 5085 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 573 245 210 170 155 120 114 67 NaeI GCC'GGC Cuts at: 2338 2338 Size: 5599 NarI GG'CG_CC Cuts at: 2214 2214 Size: 5599 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4108 Fragments arranged by size: 4108 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NgoMIV G'CCGG_C Cuts at: 2336 2336 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2438 2957 4502 1132 Size: 97 1209 519 1545 2229 Fragments arranged by size: 2229 1545 1209 519 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 1108 Size: 1416 4183 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PmlI CAC'GTG Cuts at: 2155 2155 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2536 2009 Size: 527 5072 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PstI C_TGCA'G Cuts at: 830 2141 2400 2745 2844 2861 2951 830 Size: 1311 259 345 99 17 90 3478 Fragments arranged by size: 3478 1311 345 259 99 90 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2179 2454 2521 2731 2845 2179 Size: 275 67 210 114 4933 Fragments arranged by size: 4933 275 210 114 67 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SanDI GG'GwC_CC Cuts at: 2536 2536 Size: 5599 SbfI CC_TGCA'GG Cuts at: 2951 2951 Size: 5599 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 5599 SfcI C'TryA_G Cuts at: 826 1045 2137 2396 2741 2840 2857 2947 4082 Size: 219 1092 259 345 99 17 90 1135 Cuts at: 4082 4763 4954 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1092 681 345 259 219 191 99 90 17 SfoI GGC'GCC Cuts at: 2215 2215 Size: 5599 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 3703 4604 4866 820 Size: 197 926 753 1007 901 262 1553 Fragments arranged by size: 1553 1007 926 901 753 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2438 2957 1132 Size: 1306 519 3774 Fragments arranged by size: 3774 1306 519 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 2127 1517 Size: 610 4989 StyI C'CwwG_G Cuts at: 513 1065 2004 2122 3038 513 Size: 552 939 118 916 3074 Fragments arranged by size: 3074 939 916 552 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2629 3626 3643 3796 3981 4426 325 Size: 1255 1049 997 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1049 997 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3845 5557 96 Size: 274 80 33 51 1443 1868 1712 138 Fragments arranged by size: 1868 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 5559 5591 2741 Size: 32 2786 32 2749 Fragments arranged by size: 2786 2749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2489 1316 Size: 1173 4426 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI