################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 473 GGTACC G^GTACC AflIII 110 ACATGT A^C[GA][CT]GT Alw44I 343 GTGCAC G^TGCAC AlwNI 462 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 343 GTGCAC G^TGCAC Asp718I 473 GGTACC G^GTACC BamHI 101 GGATCC G^GATCC BbrPI 340 CACGTG CAC^GTG BglII 131 AGATCT A^GATCT BsaAI 340 CACGTG [CT]AC^GT[GA] BsgI 193 GTGCAG GTGCAG(16/14) BsiEI 308 CGACCG CG[GA][CT]^CG BsmAI 107 GAGAC GTCTC(1/5) BspLU11I 110 ACATGT A^CATGT BsrI 348 CCAGT ACTGG(1/-1) BstAPI 34 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 231 GGTGACC G^GT[ACGT]ACC BstF5I 336 CATCC GGATG(2/0) BstPI 231 GGTGACC G^GT[ACGT]ACC CpoI 449 CGGTCCG CG^G[AT]CCG EarI 247 CTCTTC CTCTTC(1/4) EcoNI 360 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 231 GGTGACC G^GT[ACGT]ACC EcoT14I 119 CCAAGG C^C[AT][AT]GG FokI 336 CATCC GGATG(9/13) HphI 231 GGTGA GGTGA(8/7) KpnI 473 GGTACC GGTAC^C Ksp632I 247 CTCTTC CTCTTC(1/4) KspI 564 CCGCGG CCGC^GG MaeII 341 ACGT A^CGT MslI 336 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 110 ACATGT [GA]CATG^[CT] PmaCI 340 CACGTG CAC^GTG PmlI 340 CACGTG CAC^GTG PpuMI 356 GGGACCT [GA]G^G[AT]CC[CT] PshAI 234 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 449 CGGTCCG CG^G[AT]CCG SacII 564 CCGCGG CCGC^GG StyI 119 CCAAGG C^C[AT][AT]GG TaqI 278 TCGA T^CGA TthHB8I 278 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 114 GTAC GT^AC 474 GTAC AlwI 102 GATCC GGATC(4/5) 101 GGATC AspHI 343 GTGCAC G[AT]GC[AT]^C 670 GAGCAC AvaII 357 GGACC G^G[AT]CC 450 GGTCC BanI 40 GGTGCC G^G[CT][GA]CC 473 GGTACC BglI 481 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 563 GCCGCGGCGGC BsiHKAI 343 GTGCAC G[AT]GC[AT]^C 670 GAGCAC BsmFI 158 GGGAC GGGAC(10/14) 356 GGGAC BstYI 101 GGATCC [GA]^GATC[CT] 131 AGATCT DdeI 361 CTGAG C^T[ACGT]AG 675 CTCAG DpnII 102 GATC ^GATC 132 GATC DraII 356 GGGACCT [GA]G^G[ACGT]CC[CT] 385 GGGCCCC DsaI 530 CCGTGG C^C[GA][CT]GG 564 CCGCGG EcoO109I 356 GGGACCT [GA]G^G[ACGT]CC[CT] 385 GGGCCCC HaeII 6 GGCGCT [GA]GCGC^[CT] 656 AGCGCC MaeIII 124 GTCAC ^GT[ACGT]AC 232 GTGAC MboI 102 GATC ^GATC 132 GATC MboII 248 TCTTC GAAGA(8/7) 287 TCTTC MflI 101 GGATCC [GA]^GATC[CT] 131 AGATCT NaeI 481 GCCGGC GCC^GGC 605 GCCGGC NdeII 102 GATC ^GATC 132 GATC NgoMIV 481 GCCGGC G^CCGGC 605 GCCGGC NlaIII 111 CATG CATG^ 147 CATG RsaI 114 GTAC GT^AC 474 GTAC Sau3AI 102 GATC ^GATC 132 GATC Tsp45I 124 GTCAC ^GT[GC]AC 232 GTGAC XhoII 101 GGATCC [GA]^GATC[CT] 131 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I EaeI EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI SmaI TseI XmaCI XmaI