(Circular) (Six-base) MAPSORT of: pFN21AE6237.seq Check: 5020 from: 1 to: 5425 pFN21AE6237 5425 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2478 2746 2478 Size: 268 5157 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3488 4872 5140 1632 Size: 1856 1384 268 1917 Fragments arranged by size: 1917 1856 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 2115 4324 5301 1203 Size: 912 2209 977 1327 Fragments arranged by size: 2209 1327 977 912 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5425 AlwNI CAG_nnn'CTG Cuts at: 2472 3168 4740 2472 Size: 696 1572 3157 Fragments arranged by size: 3157 1572 696 ApaI G_GGCC'C Cuts at: 2394 2513 2649 2394 Size: 119 136 5170 Fragments arranged by size: 5170 136 119 ApaLI G'TGCA_C Cuts at: 2348 3482 4638 2348 Size: 1134 1156 3135 Fragments arranged by size: 3135 1156 1134 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2431 2648 2750 1870 Size: 73 289 199 217 102 4545 Fragments arranged by size: 4545 289 217 199 102 73 BamHI G'GATC_C Cuts at: 2106 2755 3122 2106 Size: 649 367 4409 Fragments arranged by size: 4409 649 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2478 2746 4201 618 Size: 325 217 627 258 433 268 1455 1842 Fragments arranged by size: 1842 1455 627 433 325 268 258 217 BanII G_rGCy'C Cuts at: 1391 2085 2394 2513 2649 2655 2744 1391 Size: 694 309 119 136 6 89 4072 Fragments arranged by size: 4072 694 309 136 119 89 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3616 3650 1903 Size: 34 50 34 1595 34 3678 Fragments arranged by size: 3678 1595 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 4527 3317 Size: 1210 4215 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2492 2574 Size: 107 122 71 631 605 337 483 82 Cuts at: 2574 4036 136 Size: 1462 1525 Fragments arranged by size: 1525 1462 631 605 483 337 122 107 82 71 BglII A'GATC_T Cuts at: 2136 5420 2136 Size: 3284 2141 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2352 2394 2513 2649 3486 4642 1790 Size: 562 42 119 136 837 1156 2573 Fragments arranged by size: 2573 1156 837 562 136 119 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2314 2321 2494 4085 1239 Size: 1075 7 173 1591 2579 Fragments arranged by size: 2579 1591 1075 173 7 Bpu10I CC'TnA_GC Cuts at: 2366 2366 Size: 5425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4088 882 Size: 617 2589 2219 Fragments arranged by size: 2589 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2347 3216 493 Size: 711 1143 869 2702 Fragments arranged by size: 2702 1143 869 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2220 2244 2286 Size: 53 83 186 936 522 165 24 42 Cuts at: 2286 3232 3614 4249 275 Size: 946 382 635 1451 Fragments arranged by size: 1451 946 936 635 522 382 186 165 83 53 42 24 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5200 Fragments arranged by size: 5200 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5425 BseYI C'CCAG_C Cuts at: 4628 5124 4628 Size: 496 4929 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 1163 Size: 573 483 4369 Fragments arranged by size: 4369 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2316 2875 3636 4664 1540 Size: 275 186 315 559 761 1028 2301 Fragments arranged by size: 2301 1028 761 559 315 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 2679 3486 3571 4642 2352 Size: 327 807 85 1071 3135 Fragments arranged by size: 3135 1071 807 327 85 BsmI GAATG_Cn' Cuts at: 2936 3029 2936 Size: 93 5332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2352 2394 2513 2649 2655 2679 Size: 399 295 267 42 119 136 6 24 Cuts at: 2679 2744 3486 3571 4642 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 267 136 119 85 65 42 24 6 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2780 844 Size: 1936 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3920 4094 65 Size: 1183 2672 174 1396 Fragments arranged by size: 2672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2240 2486 2610 4069 4256 1700 Size: 110 232 198 246 124 1459 187 2869 Fragments arranged by size: 2869 1459 246 232 198 187 124 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 2045 794 Size: 1251 4174 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 2236 1184 Size: 1052 4373 BstYI r'GATC_y Cuts at: 1972 2106 2136 2755 3122 3507 3524 4976 5062 Size: 134 30 619 367 385 17 1452 86 Cuts at: 5062 5074 5420 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 619 385 367 346 134 86 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2535 2569 513 Size: 552 1470 34 3369 Fragments arranged by size: 3369 1470 552 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2941 3735 3755 2941 Size: 794 20 4611 Fragments arranged by size: 4611 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2409 2493 Size: 54 1425 50 275 18 378 201 84 Cuts at: 2493 2872 3761 5390 8 Size: 379 889 1629 43 Fragments arranged by size: 1629 1425 889 379 378 275 201 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3355 1353 Size: 905 1097 3423 Fragments arranged by size: 3423 1097 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 2114 4012 4386 4532 387 Size: 1064 138 504 21 1898 374 146 1280 Fragments arranged by size: 1898 1280 1064 504 374 146 138 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2314 2321 2494 3488 4085 4872 5140 Size: 393 682 7 173 994 597 787 268 Cuts at: 5140 1239 Size: 1524 Fragments arranged by size: 1524 994 787 682 597 393 268 173 7 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 5425 EcoO109I rG'GnC_Cy Cuts at: 2362 2391 2362 Size: 29 5396 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 2015 2665 4572 1678 Size: 337 650 1907 2531 Fragments arranged by size: 2531 1907 650 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373 Size: 32 135 32 2494 32 42 32 2626 Fragments arranged by size: 2626 2494 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2482 2750 2482 Size: 268 5157 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4539 4723 1305 Size: 3234 184 2007 Fragments arranged by size: 3234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3603 Fragments arranged by size: 3603 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3383 3742 3901 518 Size: 648 1179 1038 359 159 2042 Fragments arranged by size: 2042 1179 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2407 2571 2841 3518 4666 4911 1606 Size: 552 249 164 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 270 249 245 164 NaeI GCC'GGC Cuts at: 2488 2612 2488 Size: 124 5301 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4873 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NgoMIV G'CCGG_C Cuts at: 2486 2610 2486 Size: 124 5301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2119 2783 4328 1132 Size: 97 890 664 1545 2229 Fragments arranged by size: 2229 1545 890 664 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 2115 4324 2115 Size: 2209 3216 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PmlI CAC'GTG Cuts at: 2347 2347 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5425 PshAI GACnn'nnGTC Cuts at: 1613 2243 1613 Size: 630 4795 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PspOMI G'GGCC_C Cuts at: 2390 2509 2645 2390 Size: 119 136 5170 Fragments arranged by size: 5170 136 119 PstI C_TGCA'G Cuts at: 830 2777 830 Size: 1947 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 RsrII CG'GwC_CG Cuts at: 2455 2455 Size: 5425 SacII CC_GC'GG Cuts at: 2572 2572 Size: 5425 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2773 3908 4589 4780 826 Size: 219 1728 1135 681 191 1471 Fragments arranged by size: 1728 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2234 2433 2650 2752 1872 Size: 362 199 217 102 4545 Fragments arranged by size: 4545 362 217 199 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 3529 4430 4692 820 Size: 197 926 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SrfI GCCC'GGGC Cuts at: 2650 2650 Size: 5425 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5425 StyI C'CwwG_G Cuts at: 513 1065 2124 2864 513 Size: 552 1059 740 3074 Fragments arranged by size: 3074 1059 740 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 3452 3469 3622 3807 4252 325 Size: 1255 750 1122 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1122 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3671 5383 96 Size: 274 80 33 51 1443 1694 1712 138 Fragments arranged by size: 1712 1694 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5385 5417 5385 Size: 32 5393 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2232 2431 2648 2750 1870 Size: 362 199 217 102 4545 Fragments arranged by size: 4545 362 217 199 102 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI SfoI SwaI XcmI