Restriction map for
pFN21AE6236
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BlnI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FspI HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 309 GTGCAC G^TGCAC
AlwI 203 GATCC GGATC(4/5)
ApaI 662 GGGCCC GGGCC^C
ApaLI 309 GTGCAC G^TGCAC
AspHI 309 GTGCAC G[AT]GC[AT]^C
AvaI 270 CTCGGG C^[CT]CG[GA]G
AvaII 413 GGACC G^G[AT]CC
BbrPI 103 CACGTG CAC^GTG
BclI 65 TGATCA T^GATCA
BglII 194 AGATCT A^GATCT
BlpI 36 GCTCAGC GC^T[ACGT]AGC
BpmI 229 CTCCAG CTGGAG(16/14)
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
BseRI 507 CTCCTC GAGGAG(10/8)
BsiHKAI 309 GTGCAC G[AT]GC[AT]^C
BsmAI 406 GTCTC GTCTC(1/5)
BsmBI 405 CGTCTC CGTCTC(1/5)
BsmFI 513 GTCCC GGGAC(10/14)
BsoBI 270 CTCGGG C^[CT]CG[GA]G
BspMI 624 ACCTGC ACCTGC(4/8)
BsrFI 382 GCCGGC [GA]^CCGG[CT]
BstXI 419 CCAACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 194 AGATCT [GA]^GATC[CT]
CelII 36 GCTCAGC GC^T[ACGT]AGC
Cfr10I 382 GCCGGC [GA]^CCGG[CT]
EarI 370 CTCTTC CTCTTC(1/4)
EcoT14I 323 CCAAGG C^C[AT][AT]GG
FbaI 65 TGATCA T^GATCA
FseI 381 GGCCGGCC GGCCGG^CC
HaeII 213 GGCGCT [GA]GCGC^[CT]
HapII 383 CCGG C^CGG
HpaII 383 CCGG C^CGG
HphI 68 TCACC GGTGA(8/7)
Ksp632I 370 CTCTTC CTCTTC(1/4)
MaeIII 673 GTGAC ^GT[ACGT]AC
MboII 371 TCTTC GAAGA(8/7)
MflI 194 AGATCT [GA]^GATC[CT]
MspI 383 CCGG C^CGG
NaeI 382 GCCGGC GCC^GGC
NgoMIV 382 GCCGGC G^CCGGC
PmaCI 103 CACGTG CAC^GTG
PmlI 103 CACGTG CAC^GTG
PvuII 641 CAGCTG CAG^CTG
SfaNI 179 GCATC GCATC(5/9)
SfcI 570 CTACAG C^T[GA][CT]AG
SfiI 3 GGCCAGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 323 CCAAGG C^C[AT][AT]GG
Tsp45I 673 GTGAC ^GT[GC]AC
XcmI 416 CCACCAACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 194 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 241 CGCG CG^CG
314 CGCG
AfaI 492 GTAC GT^AC
696 GTAC
AlwNI 19 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
484 CAGGCCCTG
BalI 2 TGGCCA TGG^CCA
480 TGGCCA
BanII 145 GGGCTC G[GA]GC[CT]^C
662 GGGCCC
BglI 4 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
81 GCCGCACTGGC
BsaAI 103 CACGTG [CT]AC^GT[GA]
712 TACGTG
BsgI 16 GTGCAG GTGCAG(16/14)
430 GTGCAG
BsiEI 11 CGGCCG CG[GA][CT]^CG
125 CGGCCG
BsrI 86 ACTGG ACTGG(1/-1)
186 CCAGT
BstAPI 275 GCATAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
311 GCACGCGCTGC
BstUI 241 CGCG CG^CG
314 CGCG
DdeI 37 CTCAG C^T[ACGT]AG
669 CTCAG
EagI 11 CGGCCG C^GGCCG
125 CGGCCG
EclXI 11 CGGCCG C^GGCCG
125 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
125 CGGCCG
MluNI 2 TGGCCA TGG^CCA
480 TGGCCA
MscI 2 TGGCCA TGG^CCA
480 TGGCCA
MvnI 241 CGCG CG^CG
314 CGCG
RsaI 492 GTAC GT^AC
696 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I ItaI MaeII MboI MnlI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI TspRI