Restriction map for pFN21AE1090
 (Circular) (Six-base) MAPSORT of: pFN21AE1090.seq  Check: 8345  from: 1  to: 6490



pFN21AE1090  6490 bp

 With 182 enzymes: SgfI * 

                        October 14, 2009 13:44  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4300     278 
   Size:         53      83     186     936    2764    2468

  Fragments arranged by size: 

                2764    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3833    1937 
   Size:       1896    4594
 
Acc65I G'GTAC_C

Cuts at:   3811    3811 
   Size:       6490
 
AclI AA'CG_TT

Cuts at:   1563    4617    4990    1563 
   Size:       3054     373    3063

  Fragments arranged by size: 

                3063    3054     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2866    4553    5937    6205    1632 
   Size:       1234    1687    1384     268    1917

  Fragments arranged by size: 

                1917    1687    1384    1234     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6293
 
AflIII A'CryG_T

Cuts at:   1203    2856    3565    5389    6366    1203 
   Size:       1653     709    1824     977    1327

  Fragments arranged by size: 

                1824    1653    1327     977     709
 
AgeI A'CCGG_T

Cuts at:   5321    5321 
   Size:       6490
 
AhdI GACnn_n'nnGTC

Cuts at:   5219    5219 
   Size:       6490
 
AleI CACnn'nnGTG

Cuts at:   1166    2195    2855    1166 
   Size:       1029     660    4801

  Fragments arranged by size: 

                4801    1029     660
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6490
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2374    2406    2374 
   Size:         32    6458
 
AlwNI CAG_nnn'CTG

Cuts at:   3087    3720    4233    5805    3087 
   Size:        633     513    1572    3772

  Fragments arranged by size: 

                3772    1572     633     513
 
ApaLI G'TGCA_C

Cuts at:   2186    2255    4547    5703    2186 
   Size:         69    2292    1156    2973

  Fragments arranged by size: 

                2973    2292    1156      69
 
ApoI r'AATT_y

Cuts at:   1493    2442    2984    3799    4025    4177    6286    1493 
   Size:        949     542     815     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     949     815     542     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5044     160 
   Size:        872    4012    1606

  Fragments arranged by size: 

                4012    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6490
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2363    3815    1870 
   Size:         73     420    1452    4545

  Fragments arranged by size: 

                4545    1452     420      73
 
BamHI G'GATC_C

Cuts at:   2064    2946    3732    3820    4187    2064 
   Size:        882     786      88     367    4367

  Fragments arranged by size: 

                4367     882     786     367      88
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2084    2505    2972    3432    3480 
   Size:        325     217     627     297     421     467     460      48

Cuts at:   3480    3811    5266     618 
   Size:        331    1455    1842

  Fragments arranged by size: 

                1842    1455     627     467     460     421     331     325
                 297     217      48
 
BanII G_rGCy'C

Cuts at:   1391    3600    3809    1391 
   Size:       2209     209    4072

  Fragments arranged by size: 

                4072    2209     209
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2489    2932     928 
   Size:        971     590     443    4486

  Fragments arranged by size: 

                4486     971     590     443
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4681    4715    1903 
   Size:         34      50      34    2660      34    3678

  Fragments arranged by size: 

                3678    2660      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2379    4382    5592    2379 
   Size:       2003    1210    3277

  Fragments arranged by size: 

                3277    2003    1210
 
BclI T'GATC_A

Cuts at:   1548    2109    1548 
   Size:        561    5929
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    5101     136 
   Size:        107     122      71     631     605     337    3092    1525

  Fragments arranged by size: 

                3092    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2769    6485    2769 
   Size:       3716    2774
 
BlpI GC'TnA_GC

Cuts at:   3907    3907 
   Size:       6490
 
Bme1580I G_kGCm'C

Cuts at:   1790    2190    2259    2510    4551    5707    1790 
   Size:        400      69     251    2041    1156    2573

  Fragments arranged by size: 

                2573    2041    1156     400     251      69
 
BmgBI CAC'GTC

Cuts at:   1318    3568    1318 
   Size:       2250    4240
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2785    4221    5179 
   Size:        416      53      83     399      24    1368    1436     958

Cuts at:   5179     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1436    1368     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6490
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3064    3506    3748    5150    1239 
   Size:       1825     442     242    1402    2579

  Fragments arranged by size: 

                2579    1825    1402     442     242
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4615    5480    5778    4615 
   Size:        865     298    5327

  Fragments arranged by size: 

                5327     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5153     882 
   Size:        617    3654    2219

  Fragments arranged by size: 

                3654    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2107    4281     493 
   Size:        711     903    2174    2702

  Fragments arranged by size: 

                2702    2174     903     711
 
BsaBI GATnn'nnATC

Cuts at:   6484    6484 
   Size:       6490
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4297    4679    5314     275 
   Size:         53      83     186     936    2764     382     635    1451

  Fragments arranged by size: 

                2764    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2450    2975    3735    4923    5321    5595    5742    6153    2450 
   Size:        525     760    1188     398     274     147     411    2787

  Fragments arranged by size: 

                2787    1188     760     525     411     398     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3062    3092    3479    3509    1178 
   Size:         30     165      30    1659      30     387      30    4159

  Fragments arranged by size: 

                4159    1659     387     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2237    3389    3503    1186 
   Size:       1051    1152     114    4173

  Fragments arranged by size: 

                4173    1152    1051     114
 
BseYI C'CCAG_C

Cuts at:   3713    5693    6189    3713 
   Size:       1980     496    4014

  Fragments arranged by size: 

                4014    1980     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5917
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3940    4701    5729    1540 
   Size:        275     186    1939     761    1028    2301

  Fragments arranged by size: 

                2301    1939    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2190    2259    2577    4551    4636    5707    2190 
   Size:         69     318    1974      85    1071    2973

  Fragments arranged by size: 

                2973    1974    1071     318      85      69
 
BsmI GAATG_Cn'

Cuts at:   4001    4094    4001 
   Size:         93    6397
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2039    2039 
   Size:       6490
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2190    2259    2510    2577    3600    3809    4551 
   Size:        399     400      69     251      67    1023     209     742

Cuts at:   4551    4636    5707    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071    1023     742     400     399     251     209
                  85      69      67
 
BspEI T'CCGG_A

Cuts at:   3735    6153    3735 
   Size:       2418    4072
 
BspHI T'CATG_A

Cuts at:   4379    6109    4379 
   Size:       1730    4760
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3307    3845     844 
   Size:       2463     538    3489

  Fragments arranged by size: 

                3489    2463     538
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4377    1394 
   Size:        429    2554    3507

  Fragments arranged by size: 

                3507    2554     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4985    5159      65 
   Size:       1183    3737     174    1396

  Fragments arranged by size: 

                3737    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2008    3633    5134    5321    1700 
   Size:        110     198    1625    1501     187    2869

  Fragments arranged by size: 

                2869    1625    1501     198     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3009      96 
   Size:       2913    3577
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6490
 
BssSI C'ACGA_G

Cuts at:   2617    4550    5562    2617 
   Size:       1933    1012    3545

  Fragments arranged by size: 

                3545    1933    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6490
 
BstBI TT'CG_AA

Cuts at:   5294    5294 
   Size:       6490
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6490
 
BstYI r'GATC_y

Cuts at:   1972    2064    2769    2946    3410    3732    3820    4187    4572 
   Size:         92     705     177     464     322      88     367     385

Cuts at:   4572    4589    6041    6127    6139    6485    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     705     464     385     367     346     322
                 177      92      88      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5938
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3317     487 
   Size:        683      56    2091    3660

  Fragments arranged by size: 

                3660    2091     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2032    2526    2806    4006    4800    4820    2032 
   Size:        494     280    1200     794      20    3702

  Fragments arranged by size: 

                3702    1200     794     494     280      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6490
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6455
 
DraI TTT'AAA

Cuts at:   3794    3794 
   Size:       6490
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5497     809 
   Size:        507    4181    1802

  Fragments arranged by size: 

                4181    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2602    3042    3937 
   Size:         54    1425      50     275      18     772     440     895

Cuts at:   3937    4826    6455       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     895     889     772     440     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3937    1537 
   Size:        275    2125    4090

  Fragments arranged by size: 

                4090    2125     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2520    3746    4420    1353 
   Size:       1167    1226     674    3423

  Fragments arranged by size: 

                3423    1226    1167     674
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2766    5077    5451    5597     387 
   Size:       1064     138    1177    2311     374     146    1280

  Fragments arranged by size: 

                2311    1280    1177    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2866    3064    3506    3748    4553    5150    5937 
   Size:        393    1234     198     442     242     805     597     787

Cuts at:   5937    6205    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1234     805     787     597     442     393     268
                 242     198
 
EcoO109I rG'GnC_Cy

Cuts at:   2530    3516    2530 
   Size:        986    5504
 
EcoRI G'AATT_C

Cuts at:   2442    3799    4177    2442 
   Size:       1357     378    4755

  Fragments arranged by size: 

                4755    1357     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2116    2116 
   Size:       6490
 
FspI TGC'GCA

Cuts at:   4996    4996 
   Size:       6490
 
HaeII r_GCGC'y

Cuts at:   1678    3611    3676    5637    1678 
   Size:       1933      65    1961    2531

  Fragments arranged by size: 

                2531    1961    1933      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2656    2688    3062    3094    5131 
   Size:         32     135      32    1084      32     374      32    2037

Cuts at:   5131    5163    5205    5237    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2037    1084     374     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3142    3834    4080     669 
   Size:        988     281    1204     692     246    3079

  Fragments arranged by size: 

                3079    1204     988     692     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6490
 
HpaI GTT'AAC

Cuts at:   4080    4080 
   Size:       6490
 
KpnI G_GTAC'C

Cuts at:   3815    3815 
   Size:       6490
 
MfeI C'AATT_G

Cuts at:   4089    4089 
   Size:       6490
 
MluI A'CGCG_T

Cuts at:   6366    6366 
   Size:       6490
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2716    5604    5788    1305 
   Size:       1411    2888     184    2007

  Fragments arranged by size: 

                2888    2007    1411     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4668

  Fragments arranged by size: 

                4668    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2195    2855    3233    4448    4807    4966     518 
   Size:        648    1029     660     378    1215     359     159    2042

  Fragments arranged by size: 

                2042    1215    1029     660     648     378     359     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2022    2161    2353    3001    3047    3385    3717    3726 
   Size:        416     139     192     648      46     338     332       9

Cuts at:   3726    3906    4583    5731    5976    1606 
   Size:        180     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     648     416     338     332     245
                 192     180     139      46       9
 
NaeI GCC'GGC

Cuts at:   2010    3635    2010 
   Size:       1625    4865
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5938
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6490
 
NgoMIV G'CCGG_C

Cuts at:   2008    3633    2008 
   Size:       1625    4865
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6490
 
NotI GC'GGCC_GC

Cuts at:   3937    3937 
   Size:       6490
 
NspI r_CATG'y

Cuts at:   1132    1229    3848    5393    1132 
   Size:         97    2619    1545    2229

  Fragments arranged by size: 

                2619    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6490
 
PciI A'CATG_T

Cuts at:   5389    5389 
   Size:       6490
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2094    3691    1108 
   Size:        986    1597    3907

  Fragments arranged by size: 

                3907    1597     986
 
PfoI T'CCnGG_A

Cuts at:   3361    3361 
   Size:       6490
 
PmeI GTTT'AAAC

Cuts at:   3794    3794 
   Size:       6490
 
PmlI CAC'GTG

Cuts at:   2107    2107 
   Size:       6490
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4559    4591    6072    6104    4559 
   Size:         32    1481      32    4945

  Fragments arranged by size: 

                4945    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6490
 
PsiI TTA'TAA

Cuts at:   4060    4060 
   Size:       6490
 
PstI C_TGCA'G

Cuts at:    830    2021    2027    3576    3842     830 
   Size:       1191       6    1549     266    3478

  Fragments arranged by size: 

                3478    1549    1191     266       6
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6490
 
PvuII CAG'CTG

Cuts at:   2022    3001    3385    3717    2022 
   Size:        979     384     332    4795

  Fragments arranged by size: 

                4795     979     384     332
 
SalI G'TCGA_C

Cuts at:   1936    3832    1936 
   Size:       1896    4594
 
SbfI CC_TGCA'GG

Cuts at:   3842    3842 
   Size:       6490
 
ScaI AGT'ACT

Cuts at:   2897    4738    6450    2897 
   Size:       1841    1712    2937

  Fragments arranged by size: 

                2937    1841    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2017    2023    3154    3572    3652    3838    4973 
   Size:        219     972       6    1131     418      80     186    1135

Cuts at:   4973    5654    5845     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1135    1131     972     681     418     219     191
                 186      80       6
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6490
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6490
 
SmaI CCC'GGG

Cuts at:   1872    3817    1872 
   Size:       1945    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4594    5495    5757     820 
   Size:        197     926    2651     901     262    1553

  Fragments arranged by size: 

                2651    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4281     493 
   Size:       3788    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6490
 
SphI G_CATG'C

Cuts at:   1132    3848    1132 
   Size:       2716    3774
 
SspI AAT'ATT

Cuts at:      5      52    4414       5 
   Size:         47    4362    2081

  Fragments arranged by size: 

                4362    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6490
 
StyI C'CwwG_G

Cuts at:    513    1065    2193    3929     513 
   Size:        552    1128    1736    3074

  Fragments arranged by size: 

                3074    1736    1128     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2415    3026    4517    4534    4687    4872    5317 
   Size:       1255     835     611    1491      17     153     185     445

Cuts at:   5317     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1491    1255     835     611     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2895    3009    3765 
   Size:        274      80      33      51    1443     918     114     756

Cuts at:   3765    4736    6448      96 
   Size:        971    1712     138

  Fragments arranged by size: 

                1712    1443     971     918     756     274     138     114
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3479    3511    6450    6482    3479 
   Size:         32    2939      32    3487

  Fragments arranged by size: 

                3487    2939      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6490
 
XbaI T'CTAG_A

Cuts at:   3826    3826 
   Size:       6490
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2518    3331    2518 
   Size:        813    5677
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6490
 
XmaI C'CCGG_G

Cuts at:   1870    3815    1870 
   Size:       1945    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2306    4619    2306 
   Size:       2313    4177
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4298     276 
   Size:         53      83     186     936    2764    2468

  Fragments arranged by size: 

                2764    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI     NarI     NruI
     NsiI     PacI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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