(Circular) (Six-base) MAPSORT of: pFN21AE1090.seq Check: 8345 from: 1 to: 6490 pFN21AE1090 6490 bp With 182 enzymes: SgfI * October 14, 2009 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3833 1937 Size: 1896 4594 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2866 4553 5937 6205 1632 Size: 1234 1687 1384 268 1917 Fragments arranged by size: 1917 1687 1384 1234 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 2856 3565 5389 6366 1203 Size: 1653 709 1824 977 1327 Fragments arranged by size: 1824 1653 1327 977 709 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 2195 2855 1166 Size: 1029 660 4801 Fragments arranged by size: 4801 1029 660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2374 2406 2374 Size: 32 6458 AlwNI CAG_nnn'CTG Cuts at: 3087 3720 4233 5805 3087 Size: 633 513 1572 3772 Fragments arranged by size: 3772 1572 633 513 ApaLI G'TGCA_C Cuts at: 2186 2255 4547 5703 2186 Size: 69 2292 1156 2973 Fragments arranged by size: 2973 2292 1156 69 ApoI r'AATT_y Cuts at: 1493 2442 2984 3799 4025 4177 6286 1493 Size: 949 542 815 226 152 2109 1697 Fragments arranged by size: 2109 1697 949 815 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2363 3815 1870 Size: 73 420 1452 4545 Fragments arranged by size: 4545 1452 420 73 BamHI G'GATC_C Cuts at: 2064 2946 3732 3820 4187 2064 Size: 882 786 88 367 4367 Fragments arranged by size: 4367 882 786 367 88 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2505 2972 3432 3480 Size: 325 217 627 297 421 467 460 48 Cuts at: 3480 3811 5266 618 Size: 331 1455 1842 Fragments arranged by size: 1842 1455 627 467 460 421 331 325 297 217 48 BanII G_rGCy'C Cuts at: 1391 3600 3809 1391 Size: 2209 209 4072 Fragments arranged by size: 4072 2209 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 2932 928 Size: 971 590 443 4486 Fragments arranged by size: 4486 971 590 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4681 4715 1903 Size: 34 50 34 2660 34 3678 Fragments arranged by size: 3678 2660 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2379 4382 5592 2379 Size: 2003 1210 3277 Fragments arranged by size: 3277 2003 1210 BclI T'GATC_A Cuts at: 1548 2109 1548 Size: 561 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5101 136 Size: 107 122 71 631 605 337 3092 1525 Fragments arranged by size: 3092 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2769 6485 2769 Size: 3716 2774 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 2190 2259 2510 4551 5707 1790 Size: 400 69 251 2041 1156 2573 Fragments arranged by size: 2573 2041 1156 400 251 69 BmgBI CAC'GTC Cuts at: 1318 3568 1318 Size: 2250 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2785 4221 5179 Size: 416 53 83 399 24 1368 1436 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 1753 1436 1368 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3064 3506 3748 5150 1239 Size: 1825 442 242 1402 2579 Fragments arranged by size: 2579 1825 1402 442 242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4615 5480 5778 4615 Size: 865 298 5327 Fragments arranged by size: 5327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2107 4281 493 Size: 711 903 2174 2702 Fragments arranged by size: 2702 2174 903 711 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2450 2975 3735 4923 5321 5595 5742 6153 2450 Size: 525 760 1188 398 274 147 411 2787 Fragments arranged by size: 2787 1188 760 525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 3479 3509 1178 Size: 30 165 30 1659 30 387 30 4159 Fragments arranged by size: 4159 1659 387 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 3389 3503 1186 Size: 1051 1152 114 4173 Fragments arranged by size: 4173 1152 1051 114 BseYI C'CCAG_C Cuts at: 3713 5693 6189 3713 Size: 1980 496 4014 Fragments arranged by size: 4014 1980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5917 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2259 2577 4551 4636 5707 2190 Size: 69 318 1974 85 1071 2973 Fragments arranged by size: 2973 1974 1071 318 85 69 BsmI GAATG_Cn' Cuts at: 4001 4094 4001 Size: 93 6397 BsmBI CGTCTCn'nnnn_ Cuts at: 2039 2039 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2259 2510 2577 3600 3809 4551 Size: 399 400 69 251 67 1023 209 742 Cuts at: 4551 4636 5707 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1023 742 400 399 251 209 85 69 67 BspEI T'CCGG_A Cuts at: 3735 6153 3735 Size: 2418 4072 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3307 3845 844 Size: 2463 538 3489 Fragments arranged by size: 3489 2463 538 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4985 5159 65 Size: 1183 3737 174 1396 Fragments arranged by size: 3737 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3633 5134 5321 1700 Size: 110 198 1625 1501 187 2869 Fragments arranged by size: 2869 1625 1501 198 187 110 BsrGI T'GTAC_A Cuts at: 96 3009 96 Size: 2913 3577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 2617 4550 5562 2617 Size: 1933 1012 3545 Fragments arranged by size: 3545 1933 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6490 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2064 2769 2946 3410 3732 3820 4187 4572 Size: 92 705 177 464 322 88 367 385 Cuts at: 4572 4589 6041 6127 6139 6485 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 705 464 385 367 346 322 177 92 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5938 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3317 487 Size: 683 56 2091 3660 Fragments arranged by size: 3660 2091 683 56 BtsI GCAGTG_nn' Cuts at: 2032 2526 2806 4006 4800 4820 2032 Size: 494 280 1200 794 20 3702 Fragments arranged by size: 3702 1200 794 494 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2602 3042 3937 Size: 54 1425 50 275 18 772 440 895 Cuts at: 3937 4826 6455 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 895 889 772 440 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 3746 4420 1353 Size: 1167 1226 674 3423 Fragments arranged by size: 3423 1226 1167 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2766 5077 5451 5597 387 Size: 1064 138 1177 2311 374 146 1280 Fragments arranged by size: 2311 1280 1177 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2866 3064 3506 3748 4553 5150 5937 Size: 393 1234 198 442 242 805 597 787 Cuts at: 5937 6205 1239 Size: 268 1524 Fragments arranged by size: 1524 1234 805 787 597 442 393 268 242 198 EcoO109I rG'GnC_Cy Cuts at: 2530 3516 2530 Size: 986 5504 EcoRI G'AATT_C Cuts at: 2442 3799 4177 2442 Size: 1357 378 4755 Fragments arranged by size: 4755 1357 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 2116 Size: 6490 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 3611 3676 5637 1678 Size: 1933 65 1961 2531 Fragments arranged by size: 2531 1961 1933 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 3062 3094 5131 Size: 32 135 32 1084 32 374 32 2037 Cuts at: 5131 5163 5205 5237 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2037 1084 374 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3142 3834 4080 669 Size: 988 281 1204 692 246 3079 Fragments arranged by size: 3079 1204 988 692 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6490 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2716 5604 5788 1305 Size: 1411 2888 184 2007 Fragments arranged by size: 2888 2007 1411 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4668 Fragments arranged by size: 4668 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2855 3233 4448 4807 4966 518 Size: 648 1029 660 378 1215 359 159 2042 Fragments arranged by size: 2042 1215 1029 660 648 378 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2161 2353 3001 3047 3385 3717 3726 Size: 416 139 192 648 46 338 332 9 Cuts at: 3726 3906 4583 5731 5976 1606 Size: 180 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 416 338 332 245 192 180 139 46 9 NaeI GCC'GGC Cuts at: 2010 3635 2010 Size: 1625 4865 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5938 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NgoMIV G'CCGG_C Cuts at: 2008 3633 2008 Size: 1625 4865 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 3848 5393 1132 Size: 97 2619 1545 2229 Fragments arranged by size: 2619 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2094 3691 1108 Size: 986 1597 3907 Fragments arranged by size: 3907 1597 986 PfoI T'CCnGG_A Cuts at: 3361 3361 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PmlI CAC'GTG Cuts at: 2107 2107 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PstI C_TGCA'G Cuts at: 830 2021 2027 3576 3842 830 Size: 1191 6 1549 266 3478 Fragments arranged by size: 3478 1549 1191 266 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2022 3001 3385 3717 2022 Size: 979 384 332 4795 Fragments arranged by size: 4795 979 384 332 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 2897 4738 6450 2897 Size: 1841 1712 2937 Fragments arranged by size: 2937 1841 1712 SfcI C'TryA_G Cuts at: 826 1045 2017 2023 3154 3572 3652 3838 4973 Size: 219 972 6 1131 418 80 186 1135 Cuts at: 4973 5654 5845 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1131 972 681 418 219 191 186 80 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4594 5495 5757 820 Size: 197 926 2651 901 262 1553 Fragments arranged by size: 2651 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6490 StyI C'CwwG_G Cuts at: 513 1065 2193 3929 513 Size: 552 1128 1736 3074 Fragments arranged by size: 3074 1736 1128 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2415 3026 4517 4534 4687 4872 5317 Size: 1255 835 611 1491 17 153 185 445 Cuts at: 5317 325 Size: 1498 Fragments arranged by size: 1498 1491 1255 835 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 3009 3765 Size: 274 80 33 51 1443 918 114 756 Cuts at: 3765 4736 6448 96 Size: 971 1712 138 Fragments arranged by size: 1712 1443 971 918 756 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3479 3511 6450 6482 3479 Size: 32 2939 32 3487 Fragments arranged by size: 3487 2939 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 3331 2518 Size: 813 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2306 4619 2306 Size: 2313 4177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI