(Circular) (Six-base) MAPSORT of: pFN21AB0412.seq Check: 5499 from: 1 to: 7177 pFN21AB0412 7177 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2617 4520 1937 Size: 680 1903 4594 Fragments arranged by size: 4594 1903 680 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3089 4219 5240 6624 6892 1632 Size: 1457 1130 1021 1384 268 1917 Fragments arranged by size: 1917 1457 1384 1130 1021 268 AflII C'TTAA_G Cuts at: 820 1017 2389 820 Size: 197 1372 5608 Fragments arranged by size: 5608 1372 197 AflIII A'CryG_T Cuts at: 1203 3765 6076 7053 1203 Size: 2562 2311 977 1327 Fragments arranged by size: 2562 2311 1327 977 AgeI A'CCGG_T Cuts at: 6008 6008 Size: 7177 AhdI GACnn_n'nnGTC Cuts at: 2197 3518 5906 2197 Size: 1321 2388 3468 Fragments arranged by size: 3468 2388 1321 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7177 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3906 3938 3906 Size: 32 7145 AlwNI CAG_nnn'CTG Cuts at: 2722 2911 3860 4920 6492 2722 Size: 189 949 1060 1572 3407 Fragments arranged by size: 3407 1572 1060 949 189 ApaLI G'TGCA_C Cuts at: 5234 6390 5234 Size: 1156 6021 ApoI r'AATT_y Cuts at: 1493 2747 2803 2922 3429 3456 3646 4486 4712 Size: 1254 56 119 507 27 190 840 226 Cuts at: 4712 4864 6973 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1254 840 507 226 190 152 119 56 27 AseI AT'TA_AT Cuts at: 160 1032 5731 160 Size: 872 4699 1606 Fragments arranged by size: 4699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2105 4502 1870 Size: 73 83 79 2397 4545 Fragments arranged by size: 4545 2397 83 79 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2377 2410 2377 Size: 33 7144 BamHI G'GATC_C Cuts at: 2118 4507 4874 2118 Size: 2389 367 4421 Fragments arranged by size: 4421 2389 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 4498 5953 618 Size: 325 217 627 543 2168 1455 1842 Fragments arranged by size: 2168 1842 1455 627 543 325 217 BanII G_rGCy'C Cuts at: 1391 2304 4255 4496 1391 Size: 913 1951 241 4072 Fragments arranged by size: 4072 1951 913 241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3732 4169 4221 928 Size: 971 1833 437 52 3884 Fragments arranged by size: 3884 1833 971 437 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2038 2072 5368 5402 1903 Size: 34 101 34 3296 34 3678 Fragments arranged by size: 3678 3296 101 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5069 6279 5069 Size: 1210 5967 BclI T'GATC_A Cuts at: 1548 1548 Size: 7177 BfrBI ATG'CAT Cuts at: 3187 4465 3187 Size: 1278 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5788 136 Size: 107 122 71 631 605 337 3779 1525 Fragments arranged by size: 3779 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7172 7172 Size: 7177 BlpI GC'TnA_GC Cuts at: 2064 4197 4594 2064 Size: 2133 397 4647 Fragments arranged by size: 4647 2133 397 Bme1580I G_kGCm'C Cuts at: 1790 5238 6394 1790 Size: 3448 1156 2573 Fragments arranged by size: 3448 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3620 4908 5866 Size: 416 53 83 399 24 2203 1288 958 Cuts at: 5866 442 Size: 1753 Fragments arranged by size: 2203 1753 1288 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4188 1056 Size: 3132 4045 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2275 2275 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 3972 4269 4354 5837 1239 Size: 1007 1726 297 85 1483 2579 Fragments arranged by size: 2579 1726 1483 1007 297 85 Bpu10I CC'TnA_GC Cuts at: 2035 2133 2035 Size: 98 7079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 3970 5302 6167 6465 2258 Size: 1712 1332 865 298 2970 Fragments arranged by size: 2970 1712 1332 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5840 882 Size: 617 4341 2219 Fragments arranged by size: 4341 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4968 493 Size: 711 3764 2702 Fragments arranged by size: 3764 2702 711 BsaBI GATnn'nnATC Cuts at: 2405 4311 7171 2405 Size: 1906 2860 2411 Fragments arranged by size: 2860 2411 1906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4984 5366 6001 275 Size: 53 83 186 936 3451 382 635 1451 Fragments arranged by size: 3451 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5610 6008 6282 6429 6840 5610 Size: 398 274 147 411 5947 Fragments arranged by size: 5947 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6952 Fragments arranged by size: 6952 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4340 1186 Size: 3154 4023 BseYI C'CCAG_C Cuts at: 2287 6380 6876 2287 Size: 4093 496 2588 Fragments arranged by size: 4093 2588 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2288 1163 Size: 573 363 189 6052 Fragments arranged by size: 6052 573 363 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 2219 2244 4627 5388 6416 Size: 275 186 189 29 25 2383 761 1028 Cuts at: 6416 1540 Size: 2301 Fragments arranged by size: 2383 2301 1028 761 275 189 186 29 25 BsiHKAI G_wGCw'C Cuts at: 2971 5238 5323 6394 2971 Size: 2267 85 1071 3754 Fragments arranged by size: 3754 2267 1071 85 BsmI GAATG_Cn' Cuts at: 4402 4688 4781 4402 Size: 286 93 6798 Fragments arranged by size: 6798 286 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2971 4255 4496 5238 5323 6394 Size: 399 514 667 1284 241 742 85 1071 Cuts at: 6394 1391 Size: 2174 Fragments arranged by size: 2174 1284 1071 742 667 514 399 241 85 BspEI T'CCGG_A Cuts at: 6840 6840 Size: 7177 BspHI T'CATG_A Cuts at: 5066 6796 5066 Size: 1730 5447 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 4532 844 Size: 1227 2461 3489 Fragments arranged by size: 3489 2461 1227 BsrBI CCG'CTC Cuts at: 1394 1823 2132 5064 1394 Size: 429 309 2932 3507 Fragments arranged by size: 3507 2932 429 309 BsrDI GCAATG_nn' Cuts at: 65 1248 3079 5672 5846 65 Size: 1183 1831 2593 174 1396 Fragments arranged by size: 2593 1831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2185 2218 2243 2263 5821 6008 Size: 110 249 126 33 25 20 3558 187 Cuts at: 6008 1700 Size: 2869 Fragments arranged by size: 3558 2869 249 187 126 110 33 25 20 BsrGI T'GTAC_A Cuts at: 96 2434 96 Size: 2338 4839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 3114 5237 6249 3114 Size: 2123 1012 4042 Fragments arranged by size: 4042 2123 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7177 BstBI TT'CG_AA Cuts at: 5981 5981 Size: 7177 BstEII G'GTnAC_C Cuts at: 1184 3473 1184 Size: 2289 4888 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2365 3171 2287 Size: 78 806 6293 Fragments arranged by size: 6293 806 78 BstYI r'GATC_y Cuts at: 1972 2118 2137 2561 2782 3316 4507 4874 5259 Size: 146 19 424 221 534 1191 367 385 Cuts at: 5259 5276 6728 6814 6826 7172 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1191 534 424 385 367 346 221 146 86 19 17 12 BstZ17I GTA'TAC Cuts at: 2618 2618 Size: 7177 BtgI C'CryG_G Cuts at: 513 1065 2004 2150 513 Size: 552 939 146 5540 Fragments arranged by size: 5540 939 552 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6438 Fragments arranged by size: 6438 683 56 BtsI GCAGTG_nn' Cuts at: 2049 2201 4693 5487 5507 2049 Size: 152 2492 794 20 3719 Fragments arranged by size: 3719 2492 794 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 3748 4481 3748 Size: 733 6444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6184 809 Size: 507 4868 1802 Fragments arranged by size: 4868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2187 2216 Size: 54 1425 50 275 18 243 114 29 Cuts at: 2216 2241 3205 4624 5513 7142 8 Size: 25 964 1419 889 1629 43 Fragments arranged by size: 1629 1425 1419 964 889 275 243 114 54 50 43 29 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2187 2216 2241 4624 1537 Size: 275 375 29 25 2383 4090 Fragments arranged by size: 4090 2383 375 275 29 25 EarI CTCTTCn'nnn_ Cuts at: 1353 3627 4000 4039 5107 1353 Size: 2274 373 39 1068 3423 Fragments arranged by size: 3423 2274 1068 373 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2195 5764 6138 6284 387 Size: 1064 138 606 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 606 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 3089 3972 4219 4269 4354 5240 Size: 393 614 843 883 247 50 85 886 Cuts at: 5240 5837 6624 6892 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 886 883 843 787 614 597 393 268 247 85 50 EcoO109I rG'GnC_Cy Cuts at: 2083 2256 2508 3857 2083 Size: 173 252 1349 5403 Fragments arranged by size: 5403 1349 252 173 EcoRI G'AATT_C Cuts at: 2803 2922 3646 4486 4864 2803 Size: 119 724 840 378 5116 Fragments arranged by size: 5116 840 724 378 119 EcoRV GAT'ATC Cuts at: 2986 2986 Size: 7177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4011 4039 4011 Size: 28 7149 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 2296 6324 1678 Size: 618 4028 2531 Fragments arranged by size: 4028 2531 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2398 2430 3504 Size: 32 135 32 611 32 183 32 1074 Cuts at: 3504 3536 3611 3643 3718 3750 3862 3894 4046 Size: 32 75 32 75 32 112 32 152 Cuts at: 4046 4078 5818 5850 5892 5924 1373 Size: 32 1740 32 42 32 2626 Fragments arranged by size: 2626 1740 1074 611 183 152 135 112 75 75 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4521 4767 669 Size: 988 281 1833 750 246 3079 Fragments arranged by size: 3079 1833 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 2648 2937 3548 3702 748 Size: 1900 289 611 154 4223 Fragments arranged by size: 4223 1900 611 289 154 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2539 4397 6291 6475 1305 Size: 1234 1858 1894 184 2007 Fragments arranged by size: 2007 1894 1858 1234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5355 Fragments arranged by size: 5355 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3387 5135 5494 5653 518 Size: 648 2221 1748 359 159 2042 Fragments arranged by size: 2221 2042 1748 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2287 4048 4593 5270 6418 6663 1606 Size: 546 135 1761 545 677 1148 245 2120 Fragments arranged by size: 2120 1761 1148 677 546 545 245 135 NaeI GCC'GGC Cuts at: 2061 2187 2245 2061 Size: 126 58 6993 Fragments arranged by size: 6993 126 58 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5686 Fragments arranged by size: 5686 939 552 NdeI CA'TA_TG Cuts at: 387 3388 387 Size: 3001 4176 NgoMIV G'CCGG_C Cuts at: 2059 2185 2243 2059 Size: 126 58 6993 Fragments arranged by size: 6993 126 58 NheI G'CTAG_C Cuts at: 1052 4184 1052 Size: 3132 4045 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NsiI A_TGCA'T Cuts at: 3189 4467 3189 Size: 1278 5899 NspI r_CATG'y Cuts at: 1132 1229 2072 3187 4535 6080 1132 Size: 97 843 1115 1348 1545 2229 Fragments arranged by size: 2229 1545 1348 1115 843 97 PasI CC'CwG_GG Cuts at: 1348 3860 1348 Size: 2512 4665 PciI A'CATG_T Cuts at: 6076 6076 Size: 7177 PflMI CCAn_nnn'nTGG Cuts at: 1108 4106 1108 Size: 2998 4179 PfoI T'CCnGG_A Cuts at: 2670 2670 Size: 7177 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5246 5278 6759 6791 5246 Size: 32 1481 32 5632 Fragments arranged by size: 5632 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2508 2083 Size: 425 6752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 4747 4747 Size: 7177 PstI C_TGCA'G Cuts at: 830 4529 830 Size: 3699 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 4048 4048 Size: 7177 RsrII CG'GwC_CG Cuts at: 2127 2127 Size: 7177 SacII CC_GC'GG Cuts at: 2153 2153 Size: 7177 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SanDI GG'GwC_CC Cuts at: 2508 2508 Size: 7177 SapI GCTCTTCn'nnn_ Cuts at: 3627 4039 3627 Size: 412 6765 SbfI CC_TGCA'GG Cuts at: 4529 4529 Size: 7177 ScaI AGT'ACT Cuts at: 4331 5425 7137 4331 Size: 1094 1712 4371 Fragments arranged by size: 4371 1712 1094 SexAI A'CCwGG_T Cuts at: 3690 4473 3690 Size: 783 6394 SfcI C'TryA_G Cuts at: 826 1045 4345 4525 5660 6341 6532 826 Size: 219 3300 180 1135 681 191 1471 Fragments arranged by size: 3300 1471 1135 681 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 2263 1700 Size: 563 6614 SmaI CCC'GGG Cuts at: 1872 2107 4504 1872 Size: 235 2397 4545 Fragments arranged by size: 4545 2397 235 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 2389 3985 5281 6182 6444 Size: 197 926 330 116 1596 1296 901 262 Cuts at: 6444 820 Size: 1553 Fragments arranged by size: 1596 1553 1296 926 901 330 262 197 116 SnaBI TAC'GTA Cuts at: 493 4968 493 Size: 4475 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 4535 1132 Size: 3403 3774 SspI AAT'ATT Cuts at: 5 52 4364 5101 5 Size: 47 4312 737 2081 Fragments arranged by size: 4312 2081 737 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7177 StyI C'CwwG_G Cuts at: 513 1065 2004 4616 513 Size: 552 939 2612 3074 Fragments arranged by size: 3074 2612 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5204 5221 5374 5559 6004 325 Size: 1255 3624 17 153 185 445 1498 Fragments arranged by size: 3624 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2434 2772 4329 Size: 274 80 33 51 1443 457 338 1557 Cuts at: 4329 5423 7135 96 Size: 1094 1712 138 Fragments arranged by size: 1712 1557 1443 1094 457 338 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2329 2361 3470 3502 4108 4137 4140 4169 7137 Size: 32 1109 32 606 29 3 29 2968 Cuts at: 7137 7169 2329 Size: 32 2337 Fragments arranged by size: 2968 2337 1109 606 32 32 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 3491 4513 3491 Size: 1022 6155 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 3572 2362 Size: 1210 5967 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7177 XmaI C'CCGG_G Cuts at: 1870 2105 4502 1870 Size: 235 2397 4545 Fragments arranged by size: 4545 2397 235 XmnI GAAnn'nnTTC Cuts at: 3499 3994 5306 3499 Size: 495 1312 5370 Fragments arranged by size: 5370 1312 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI SacI SfiI SfoI SrfI SwaI