(Linear) (Six-base) MAPSORT of: KIBB0412.seq Check: 7766 from: 1 to: 2472 KIBB0412.orf With 182 enzymes: SgfI * July 3, 2012 16:00 .. AccI GT'mk_AC Cuts at: 0 612 2472 Size: 612 1860 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1084 2214 2472 Size: 1084 1130 258 Fragments arranged by size: 1130 1084 258 AflII C'TTAA_G Cuts at: 0 384 2472 Size: 384 2088 AflIII A'CryG_T Cuts at: 0 1760 2472 Size: 1760 712 AhdI GACnn_n'nnGTC Cuts at: 0 192 1513 2472 Size: 192 1321 959 Fragments arranged by size: 1321 959 192 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1901 1933 2472 Size: 1901 32 539 Fragments arranged by size: 1901 539 32 AlwNI CAG_nnn'CTG Cuts at: 0 717 906 1855 2472 Size: 717 189 949 617 Fragments arranged by size: 949 717 617 189 ApoI r'AATT_y Cuts at: 0 742 798 917 1424 1451 1641 2472 Size: 742 56 119 507 27 190 831 Fragments arranged by size: 831 742 507 190 119 56 27 AvaI C'yCGr_G Cuts at: 0 21 100 2472 Size: 21 79 2372 Fragments arranged by size: 2372 79 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 372 405 2472 Size: 372 33 2067 Fragments arranged by size: 2067 372 33 BamHI G'GATC_C Cuts at: 0 113 2472 Size: 113 2359 BanI G'GyrC_C Cuts at: 0 325 2472 Size: 325 2147 BanII G_rGCy'C Cuts at: 0 299 2250 2472 Size: 299 1951 222 Fragments arranged by size: 1951 299 222 BbsI GAAGACnn'nnnn_ Cuts at: 0 1727 2164 2216 2472 Size: 1727 437 52 256 Fragments arranged by size: 1727 437 256 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 33 67 2472 Size: 33 34 2405 Fragments arranged by size: 2405 34 33 BfrBI ATG'CAT Cuts at: 0 1182 2460 2472 Size: 1182 1278 12 Fragments arranged by size: 1278 1182 12 BlpI GC'TnA_GC Cuts at: 0 59 2192 2472 Size: 59 2133 280 Fragments arranged by size: 2133 280 59 BmrI ACTGGGnnnn_n' Cuts at: 0 1615 2472 Size: 1615 857 BmtI G_CTAG'C Cuts at: 0 2183 2472 Size: 2183 289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 270 2472 Size: 270 2202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 1967 2264 2349 2472 Size: 241 1726 297 85 123 Fragments arranged by size: 1726 297 241 123 85 Bpu10I CC'TnA_GC Cuts at: 0 30 128 2472 Size: 30 98 2344 Fragments arranged by size: 2344 98 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1965 2472 Size: 253 1712 507 Fragments arranged by size: 1712 507 253 BsaBI GATnn'nnATC Cuts at: 0 400 2306 2472 Size: 400 1906 166 Fragments arranged by size: 1906 400 166 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2335 2472 Size: 2335 137 BseYI C'CCAG_C Cuts at: 0 282 2472 Size: 282 2190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 283 2472 Size: 94 189 2189 Fragments arranged by size: 2189 189 94 BsiEI CG_ry'CG Cuts at: 0 185 214 239 2472 Size: 185 29 25 2233 Fragments arranged by size: 2233 185 29 25 BsiHKAI G_wGCw'C Cuts at: 0 966 2472 Size: 966 1506 BsmI GAATG_Cn' Cuts at: 0 2397 2472 Size: 2397 75 Bsp1286I G_dGCh'C Cuts at: 0 299 966 2250 2472 Size: 299 667 1284 222 Fragments arranged by size: 1284 667 299 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 66 2472 Size: 66 2406 BsrBI CCG'CTC Cuts at: 0 127 2472 Size: 127 2345 BsrDI GCAATG_nn' Cuts at: 0 1074 2472 Size: 1074 1398 BsrFI r'CCGG_y Cuts at: 0 54 180 213 238 258 2472 Size: 54 126 33 25 20 2214 Fragments arranged by size: 2214 126 54 33 25 20 BsrGI T'GTAC_A Cuts at: 0 429 2472 Size: 429 2043 BssSI C'ACGA_G Cuts at: 0 1109 2472 Size: 1109 1363 BstEII G'GTnAC_C Cuts at: 0 1468 2472 Size: 1468 1004 BstXI CCAn_nnnn'nTGG Cuts at: 0 282 360 1166 2472 Size: 282 78 806 1306 Fragments arranged by size: 1306 806 282 78 BstYI r'GATC_y Cuts at: 0 113 132 556 777 1311 2472 Size: 113 19 424 221 534 1161 Fragments arranged by size: 1161 534 424 221 113 19 BstZ17I GTA'TAC Cuts at: 0 613 2472 Size: 613 1859 BtgI C'CryG_G Cuts at: 0 145 2472 Size: 145 2327 BtsI GCAGTG_nn' Cuts at: 0 44 196 2472 Size: 44 152 2276 Fragments arranged by size: 2276 152 44 DraI TTT'AAA Cuts at: 0 1743 2472 Size: 1743 729 EaeI y'GGCC_r Cuts at: 0 68 182 211 236 1200 2472 Size: 68 114 29 25 964 1272 Fragments arranged by size: 1272 964 114 68 29 25 EagI C'GGCC_G Cuts at: 0 182 211 236 2472 Size: 182 29 25 2236 Fragments arranged by size: 2236 182 29 25 EarI CTCTTCn'nnn_ Cuts at: 0 1622 1995 2034 2472 Size: 1622 373 39 438 Fragments arranged by size: 1622 438 373 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 190 2472 Size: 190 2282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 1084 1967 2214 2264 2349 2472 Size: 241 843 883 247 50 85 123 Fragments arranged by size: 883 843 247 241 123 85 50 EcoO109I rG'GnC_Cy Cuts at: 0 78 251 503 1852 2472 Size: 78 173 252 1349 620 Fragments arranged by size: 1349 620 252 173 78 EcoRI G'AATT_C Cuts at: 0 798 917 1641 2472 Size: 798 119 724 831 Fragments arranged by size: 831 798 724 119 EcoRV GAT'ATC Cuts at: 0 981 2472 Size: 981 1491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2006 2034 2472 Size: 2006 28 438 Fragments arranged by size: 2006 438 28 HaeII r_GCGC'y Cuts at: 0 291 2472 Size: 291 2181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 178 210 393 425 1499 1531 1606 1638 Size: 178 32 183 32 1074 32 75 32 Cuts at: 1638 1713 1745 1857 1889 2041 2073 2472 Size: 75 32 112 32 152 32 399 Fragments arranged by size: 1074 399 183 178 152 112 75 75 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1766 2472 Size: 1766 706 HindIII A'AGCT_T Cuts at: 0 643 932 1543 1697 2472 Size: 643 289 611 154 775 Fragments arranged by size: 775 643 611 289 154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 534 2392 2472 Size: 534 1858 80 Fragments arranged by size: 1858 534 80 MslI CAynn'nnrTG Cuts at: 0 1382 2472 Size: 1382 1090 MspA1I CmG'CkG Cuts at: 0 147 282 2043 2472 Size: 147 135 1761 429 Fragments arranged by size: 1761 429 147 135 NaeI GCC'GGC Cuts at: 0 56 182 240 2472 Size: 56 126 58 2232 Fragments arranged by size: 2232 126 58 56 NdeI CA'TA_TG Cuts at: 0 1383 2472 Size: 1383 1089 NgoMIV G'CCGG_C Cuts at: 0 54 180 238 2472 Size: 54 126 58 2234 Fragments arranged by size: 2234 126 58 54 NheI G'CTAG_C Cuts at: 0 2179 2472 Size: 2179 293 NsiI A_TGCA'T Cuts at: 0 1184 2462 2472 Size: 1184 1278 10 Fragments arranged by size: 1278 1184 10 NspI r_CATG'y Cuts at: 0 67 1182 2472 Size: 67 1115 1290 Fragments arranged by size: 1290 1115 67 PasI CC'CwG_GG Cuts at: 0 1855 2472 Size: 1855 617 PflMI CCAn_nnn'nTGG Cuts at: 0 2101 2472 Size: 2101 371 PfoI T'CCnGG_A Cuts at: 0 665 2472 Size: 665 1807 PpuMI rG'GwC_Cy Cuts at: 0 78 503 2472 Size: 78 425 1969 Fragments arranged by size: 1969 425 78 PvuII CAG'CTG Cuts at: 0 2043 2472 Size: 2043 429 RsrII CG'GwC_CG Cuts at: 0 122 2472 Size: 122 2350 SacII CC_GC'GG Cuts at: 0 148 2472 Size: 148 2324 SanDI GG'GwC_CC Cuts at: 0 503 2472 Size: 503 1969 SapI GCTCTTCn'nnn_ Cuts at: 0 1622 2034 2472 Size: 1622 412 438 Fragments arranged by size: 1622 438 412 ScaI AGT'ACT Cuts at: 0 2326 2472 Size: 2326 146 SexAI A'CCwGG_T Cuts at: 0 1685 2472 Size: 1685 787 SfcI C'TryA_G Cuts at: 0 2340 2472 Size: 2340 132 SgrAI Cr'CCGG_yG Cuts at: 0 258 2472 Size: 258 2214 SmaI CCC'GGG Cuts at: 0 102 2472 Size: 102 2370 SmlI C'TyrA_G Cuts at: 0 268 384 1980 2472 Size: 268 116 1596 492 Fragments arranged by size: 1596 492 268 116 SspI AAT'ATT Cuts at: 0 2359 2472 Size: 2359 113 TatI w'GTAC_w Cuts at: 0 429 767 2324 2472 Size: 429 338 1557 148 Fragments arranged by size: 1557 429 338 148 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 324 356 1465 1497 2103 2132 2135 2164 Size: 324 32 1109 32 606 29 3 29 Cuts at: 2164 2472 Size: 308 Fragments arranged by size: 1109 606 324 308 32 32 29 29 3 XbaI T'CTAG_A Cuts at: 0 1486 2472 Size: 1486 986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 357 1567 2472 Size: 357 1210 905 Fragments arranged by size: 1210 905 357 XmaI C'CCGG_G Cuts at: 0 100 2472 Size: 100 2372 XmnI GAAnn'nnTTC Cuts at: 0 1494 1989 2472 Size: 1494 495 483 Fragments arranged by size: 1494 495 483 Enzymes that do cut: AccI AcuI AflII AflIII AhdI AloI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbsI BcgI BfrBI BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI BstZ17I BtgI BtsI DraI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NaeI NdeI NgoMIV NheI NsiI NspI PasI PflMI PfoI PpuMI PvuII RsrII SacII SanDI SapI ScaI SexAI SfcI SgrAI SmaI SmlI SspI TatI TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BciVI BclI BglI BglII Bme1580I BmgBI BsaI BsaAI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BssHII BstAPI BstBI Bsu36I BtgZI ClaI CspCI DraIII DrdI EcoICRI EcoNI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI NarI NcoI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SbfI SfiI SfoI SgfI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I XhoI ZraI