################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsgI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspDI BspHI BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MseI MspA1I MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AluI 839 AGCT AG^CT AlwI 461 GGATC GGATC(4/5) Asp700I 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 428 GGTGCC G^G[CT][GA]CC BciVI 51 GGATAC GTATCC(6/5) BglI 448 GCCGGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 34 GGGCAC G[AGT]GC[ACT]^C BpmI 802 CTGGAG CTGGAG(16/14) BsaAI 898 TACGTG [CT]AC^GT[GA] BsiEI 396 CGACCG CG[GA][CT]^CG BsmAI 856 GTCTC GTCTC(1/5) BsmI 187 GCATTC GAATGC(1/-1) Bsp1286I 34 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 288 TGTACA T^GTACA BspLU11I 356 ACATGT A^CATGT BsrDI 161 GCAATG GCAATG(2/0) BsrGI 288 TGTACA T^GTACA DpnII 462 GATC ^GATC DrdI 969 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 973 GAAGAG CTCTTC(1/4) EcoT14I 119 CCTTGG C^C[AT][AT]GG FseI 906 GGCCGGCC GGCCGG^CC HincII 966 GTTGAC GT[CT]^[GA]AC HindII 966 GTTGAC GT[CT]^[GA]AC Ksp632I 973 GAAGAG CTCTTC(1/4) MboI 462 GATC ^GATC NdeII 462 GATC ^GATC NlaIII 357 CATG CATG^ NspI 356 ACATGT [GA]CATG^[CT] PstI 324 CTGCAG CTGCA^G Sau3AI 462 GATC ^GATC ScaI 539 AGTACT AGT^ACT SexAI 773 ACCAGGT A^CC[AT]GGT SfiI 447 GGCCGGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 721 CTCAAG C^T[CT][GA]AG SspBI 288 TGTACA T^GTACA StyI 119 CCTTGG C^C[AT][AT]GG TaqI 191 TCGA T^CGA Tsp509I 13 AATT ^AATT TthHB8I 191 TCGA T^CGA XmnI 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 704 CGCG CG^CG 1007 CGCG AccIII 680 TCCGGA T^CCGGA 956 TCCGGA AflIII 356 ACATGT A^C[GA][CT]GT 962 ACGTGT BbsI 1035 GAAGAC GAAGAC(2/6) 139 GTCTTC BcnI 522 CCGGG CC^[GC]GG 531 CCGGG BpuAI 1035 GAAGAC GAAGAC(2/6) 139 GTCTTC BsaWI 680 TCCGGA [AT]^CCGG[AT] 956 TCCGGA BseAI 680 TCCGGA T^CCGGA 956 TCCGGA BseRI 952 CTCCTC GAGGAG(10/8) 535 GAGGAG BspEI 680 TCCGGA T^CCGGA 956 TCCGGA BsrFI 448 GCCGGC [GA]^CCGG[CT] 907 GCCGGC BsrI 302 ACTGG ACTGG(1/-1) 746 CCAGT BstUI 704 CGCG CG^CG 1007 CGCG Cfr10I 448 GCCGGC [GA]^CCGG[CT] 907 GCCGGC FauI 314 GCGGG CCCGC(4/6) 591 GCGGG HaeII 261 GGCGCT [GA]GCGC^[CT] 391 AGCGCC HinfI 844 GACTC G^A[ACGT]TC 976 GAGTC MroI 680 TCCGGA T^CCGGA 956 TCCGGA MvnI 704 CGCG CG^CG 1007 CGCG NaeI 448 GCCGGC GCC^GGC 907 GCCGGC NciI 522 CCGGG CC^[GC]GG 531 CCGGG NgoMIV 448 GCCGGC G^CCGGC 907 GCCGGC PleI 844 GACTC GAGTC(4/5) 976 GAGTC SfcI 324 CTGCAG C^T[GA][CT]AG 573 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AvaII BalI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MluNI MnlI MscI MslI MspI MvaI MwoI NlaIV RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XcmI