(Linear) (Six-base) MAPSORT of: KIEE9574.seq Check: 5819 from: 1 to: 1050 KIEE9574.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 992 1050 Size: 992 58 AflIII A'CryG_T Cuts at: 0 356 962 1050 Size: 356 606 88 Fragments arranged by size: 606 356 88 AleI CACnn'nnGTG Cuts at: 0 73 1050 Size: 73 977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 349 1050 Size: 349 701 BanI G'GyrC_C Cuts at: 0 428 1050 Size: 428 622 BbsI GAAGACnn'nnnn_ Cuts at: 0 132 1042 1050 Size: 132 910 8 Fragments arranged by size: 910 132 8 BciVI GTATCCnnnnn_n' Cuts at: 0 45 1050 Size: 45 1005 BglI GCCn_nnn'nGGC Cuts at: 0 454 1050 Size: 454 596 Bme1580I G_kGCm'C Cuts at: 0 38 1050 Size: 38 1012 BmgBI CAC'GTC Cuts at: 0 281 686 1050 Size: 281 405 364 Fragments arranged by size: 405 364 281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 823 1050 Size: 823 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 1050 Size: 706 344 BsaAI yAC'GTr Cuts at: 0 900 1050 Size: 900 150 BsaWI w'CCGG_w Cuts at: 0 680 956 1050 Size: 680 276 94 Fragments arranged by size: 680 276 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 235 265 796 826 982 1012 1050 Size: 235 30 531 30 156 30 38 Fragments arranged by size: 531 235 156 38 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 550 943 1050 Size: 550 393 107 Fragments arranged by size: 550 393 107 BseYI C'CCAG_C Cuts at: 0 156 946 1050 Size: 156 790 104 Fragments arranged by size: 790 156 104 BsiEI CG_ry'CG Cuts at: 0 399 1050 Size: 399 651 BsmI GAATG_Cn' Cuts at: 0 187 1050 Size: 187 863 Bsp1286I G_dGCh'C Cuts at: 0 38 1050 Size: 38 1012 BspEI T'CCGG_A Cuts at: 0 680 956 1050 Size: 680 276 94 Fragments arranged by size: 680 276 94 BsrDI GCAATG_nn' Cuts at: 0 168 1050 Size: 168 882 BsrFI r'CCGG_y Cuts at: 0 448 907 1050 Size: 448 459 143 Fragments arranged by size: 459 448 143 BsrGI T'GTAC_A Cuts at: 0 288 1050 Size: 288 762 BtgI C'CryG_G Cuts at: 0 473 602 980 1050 Size: 473 129 378 70 Fragments arranged by size: 473 378 129 70 BtsI GCAGTG_nn' Cuts at: 0 743 1050 Size: 743 307 DrdI GACnn_nn'nnGTC Cuts at: 0 975 1050 Size: 975 75 EaeI y'GGCC_r Cuts at: 0 380 605 743 905 909 1050 Size: 380 225 138 162 4 141 Fragments arranged by size: 380 225 162 141 138 4 EarI CTCTTCn'nnn_ Cuts at: 0 968 1050 Size: 968 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 823 992 1050 Size: 823 169 58 Fragments arranged by size: 823 169 58 FseI GG_CCGG'CC Cuts at: 0 911 1050 Size: 911 139 HaeII r_GCGC'y Cuts at: 0 265 395 1050 Size: 265 130 655 Fragments arranged by size: 655 265 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 235 267 796 828 982 1014 1050 Size: 235 32 529 32 154 32 36 Fragments arranged by size: 529 235 154 36 32 32 32 HincII GTy'rAC Cuts at: 0 968 1050 Size: 968 82 MscI TGG'CCA Cuts at: 0 382 607 745 1050 Size: 382 225 138 305 Fragments arranged by size: 382 305 225 138 MslI CAynn'nnrTG Cuts at: 0 41 73 898 1050 Size: 41 32 825 152 Fragments arranged by size: 825 152 41 32 NaeI GCC'GGC Cuts at: 0 450 909 1050 Size: 450 459 141 Fragments arranged by size: 459 450 141 NgoMIV G'CCGG_C Cuts at: 0 448 907 1050 Size: 448 459 143 Fragments arranged by size: 459 448 143 NspI r_CATG'y Cuts at: 0 360 1050 Size: 360 690 PciI A'CATG_T Cuts at: 0 356 1050 Size: 356 694 PfoI T'CCnGG_A Cuts at: 0 530 800 1050 Size: 530 270 250 Fragments arranged by size: 530 270 250 PstI C_TGCA'G Cuts at: 0 328 1050 Size: 328 722 ScaI AGT'ACT Cuts at: 0 541 1050 Size: 541 509 SexAI A'CCwGG_T Cuts at: 0 773 1050 Size: 773 277 SfcI C'TryA_G Cuts at: 0 324 573 1050 Size: 324 249 477 Fragments arranged by size: 477 324 249 SfiI GGCCn_nnn'nGGCC Cuts at: 0 454 1050 Size: 454 596 SmlI C'TyrA_G Cuts at: 0 721 1050 Size: 721 329 StyI C'CwwG_G Cuts at: 0 119 1050 Size: 119 931 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 168 831 1050 Size: 168 663 219 Fragments arranged by size: 663 219 168 TatI w'GTAC_w Cuts at: 0 288 539 1050 Size: 288 251 511 Fragments arranged by size: 511 288 251 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 453 485 806 835 838 867 1050 Size: 453 32 321 29 3 29 183 Fragments arranged by size: 453 321 183 32 29 29 3 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 307 615 897 1050 Size: 307 308 282 153 Fragments arranged by size: 308 307 282 153 XmnI GAAnn'nnTTC Cuts at: 0 7 1050 Size: 7 1043 Enzymes that do cut: AcuI AflIII AleI AlfI BanI BbsI BciVI BglI Bme1580I BmgBI BpmI BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsmI Bsp1286I BspEI BsrDI BsrFI BsrGI BtgI BtsI DrdI EaeI EarI Eco57MI FseI HaeII Hin4I HincII MscI MslI NaeI NgoMIV NspI PciI PfoI PstI ScaI SexAI SfcI SfiI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsgI BsiHKAI BsiWI BsmBI BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI