################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MaeII MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 524 GGTACC G^GTACC AcsI 469 GAATTT [GA]^AATT[CT] AlwNI 736 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1051 CCTAAGG CC^T[ACGT]AGG ApoI 469 GAATTT [GA]^AATT[CT] Asp718I 524 GGTACC G^GTACC AspHI 592 GAGCTC G[AT]GC[AT]^C AvaI 85 CCCGAG C^[CT]CG[GA]G BamHI 1056 GGATCC G^GATCC BbeI 148 GGCGCC GGCGC^C BbsI 658 GTCTTC GAAGAC(2/6) BcgI 1096 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 216 CCGGG CC^[GC]GG BlpI 943 GCTCAGC GC^T[ACGT]AGC Bpu1102I 943 GCTCAGC GC^T[ACGT]AGC BpuAI 658 GTCTTC GAAGAC(2/6) BseRI 1039 GAGGAG GAGGAG(10/8) BsiEI 70 CGGCCG CG[GA][CT]^CG BsiHKAI 592 GAGCTC G[AT]GC[AT]^C BsiWI 162 CGTACG C^GTACG BsmI 183 GAATGC GAATGC(1/-1) BsoBI 85 CCCGAG C^[CT]CG[GA]G BspHI 371 TCATGA T^CATGA BspMI 527 ACCTGC ACCTGC(4/8) BsrDI 816 CATTGC GCAATG(2/0) BstAPI 617 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1051 CCTAAGG CC^T[ACGT]AGG CelII 943 GCTCAGC GC^T[ACGT]AGC DraII 482 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 174 CCGCGG C^C[GA][CT]GG EagI 70 CGGCCG C^GGCCG EclXI 70 CGGCCG C^GGCCG Eco52I 70 CGGCCG C^GGCCG Eco81I 1051 CCTAAGG CC^T[ACGT]AGG EcoO109I 482 AGGACCT [GA]G^G[ACGT]CC[CT] HapII 216 CCGG C^CGG HgaI 518 GCGTC GACGC(5/10) HpaII 216 CCGG C^CGG KasI 148 GGCGCC G^GCGCC KpnI 524 GGTACC GGTAC^C KspI 174 CCGCGG CCGC^GG MseI 581 TTAA T^TAA MslI 675 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 216 CCGG C^CGG NarI 148 GGCGCC GG^CGCC NciI 216 CCGGG CC^[GC]GG NotI 69 GCGGCCGC GC^GGCCGC PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT] RcaI 371 TCATGA T^CATGA SacI 592 GAGCTC GAGCT^C SacII 174 CCGCGG CCGC^GG SapI 420 GCTCTTC GCTCTTC(1/4) SfoI 148 GGCGCC GGC^GCC SphI 939 GCATGC GCATG^C SspI 282 AATATT AAT^ATT StuI 753 AGGCCT AGG^CCT Tru9I 581 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 148 GGCGCC G[GA]^CG[CT]C 517 GGCGTC AvaII 483 GGACC G^G[AT]CC 966 GGTCC BalI 695 TGGCCA TGG^CCA 1004 TGGCCA BanII 262 GGGCTC G[GA]GC[CT]^C 592 GAGCTC BbvCI 12 CCTCAGC CCTCAGC(-5/-2) 531 GCTGAGG BmyI 262 GGGCTC G[AGT]GC[ACT]^C 592 GAGCTC BpmI 745 CTGGAG CTGGAG(16/14) 1114 CTGGAG BsaHI 148 GGCGCC G[GA]^CG[CT]C 517 GGCGTC BsgI 249 CTGCAC GTGCAG(16/14) 598 CTGCAC Bsp1286I 262 GGGCTC G[AGT]GC[ACT]^C 592 GAGCTC BsrI 542 ACTGG ACTGG(1/-1) 619 ACTGG BstF5I 627 CATCC GGATG(2/0) 219 GGATG BstXI 479 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 677 CCAATGTGATGG EcoT14I 479 CCAAGG C^C[AT][AT]GG 1007 CCAAGG FokI 627 CATCC GGATG(9/13) 219 GGATG Hin1I 148 GGCGCC G[GA]^CG[CT]C 517 GGCGTC HphI 687 GGTGA GGTGA(8/7) 209 TCACC MluNI 695 TGGCCA TGG^CCA 1004 TGGCCA MscI 695 TGGCCA TGG^CCA 1004 TGGCCA NspI 194 ACATGC [GA]CATG^[CT] 939 GCATGC PvuII 246 CAGCTG CAG^CTG 415 CAGCTG SexAI 485 ACCTGGT A^CC[AT]GGT 962 ACCTGGT StyI 479 CCAAGG C^C[AT][AT]GG 1007 CCAAGG TfiI 338 GAATC G^A[AT]TC 1075 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanI BbvI BsaJI BsiYI BslI BsmFI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI HaeII HaeIII HhaI HinP1I HinfI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII