Restriction map for pFN21AE6351
 (Circular) (Six-base) MAPSORT of: pFN21AE6351.seq  Check: 5709  from: 1  to: 5836



pFN21AE6351  5836 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3646     278 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3179    1937 
   Size:       1242    4594
 
Acc65I G'GTAC_C

Cuts at:   2529    3157    2529 
   Size:        628    5208
 
AclI AA'CG_TT

Cuts at:   1563    3963    4336    1563 
   Size:       2400     373    3063

  Fragments arranged by size: 

                3063    2400     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2178    3899    5283    5551    1632 
   Size:        546    1721    1384     268    1917

  Fragments arranged by size: 

                1917    1721    1384     546     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5639
 
AflIII A'CryG_T

Cuts at:   1203    4735    5712    1203 
   Size:       3532     977    1327

  Fragments arranged by size: 

                3532    1327     977
 
AgeI A'CCGG_T

Cuts at:   4667    4667 
   Size:       5836
 
AhdI GACnn_n'nnGTC

Cuts at:   4565    4565 
   Size:       5836
 
AleI CACnn'nnGTG

Cuts at:   1166    2684    1166 
   Size:       1518    4318
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2138    2568    1184 
   Size:        954     430    4452

  Fragments arranged by size: 

                4452     954     430
 
AlwNI CAG_nnn'CTG

Cuts at:   2746    3579    5151    2746 
   Size:        833    1572    3431

  Fragments arranged by size: 

                3431    1572     833
 
ApaLI G'TGCA_C

Cuts at:   3893    5049    3893 
   Size:       1156    4680
 
ApoI r'AATT_y

Cuts at:   1493    2474    3145    3371    3523    5632    1493 
   Size:        981     671     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     981     671     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4390     160 
   Size:        872    3358    1606

  Fragments arranged by size: 

                3358    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2090    3161    1870 
   Size:         73     147    1071    4545

  Fragments arranged by size: 

                4545    1071     147      73
 
BamHI G'GATC_C

Cuts at:   3061    3166    3533    3061 
   Size:        105     367    5364

  Fragments arranged by size: 

                5364     367     105
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2153    2529    2678    2716    3157 
   Size:        325     217     627     366     376     149      38     441

Cuts at:   3157    4612     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455     627     441     376     366     325     217
                 149      38
 
BanII G_rGCy'C

Cuts at:   1391    2271    2601    3155    1391 
   Size:        880     330     554    4072

  Fragments arranged by size: 

                4072     880     554     330
 
BbeI G_GCGC'C

Cuts at:   2157    2157 
   Size:       5836
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2656     928 
   Size:        971     757    4108

  Fragments arranged by size: 

                4108     971     757
 
BbvCI CC'TCA_GC

Cuts at:   2018    2537    2018 
   Size:        519    5317
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3090    3124    4027    4061    1903 
   Size:         34    1153      34     903      34    3678

  Fragments arranged by size: 

                3678    1153     903      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3728    4938    3728 
   Size:       1210    4626
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5836
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4447     136 
   Size:        107     122      71     631     605    2775    1525

  Fragments arranged by size: 

                2775    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5831    5831 
   Size:       5836
 
BlpI GC'TnA_GC

Cuts at:   2949    3253    2949 
   Size:        304    5532
 
Bme1580I G_kGCm'C

Cuts at:   1790    3897    5053    1790 
   Size:       2107    1156    2573

  Fragments arranged by size: 

                2573    2107    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5836
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3567    4525     442 
   Size:        416      53      83     399      24    2150     958    1753

  Fragments arranged by size: 

                2150    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5836
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2615    2615 
   Size:       5836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2771    3140    4496    1239 
   Size:       1532     369    1356    2579

  Fragments arranged by size: 

                2579    1532    1356     369
 
Bpu10I CC'TnA_GC

Cuts at:   2018    2507    2537    2018 
   Size:        489      30    5317

  Fragments arranged by size: 

                5317     489      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3961    4826    5124    3961 
   Size:        865     298    4673

  Fragments arranged by size: 

                4673     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4499     882 
   Size:        617    3000    2219

  Fragments arranged by size: 

                3000    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3627     493 
   Size:        711    2423    2702

  Fragments arranged by size: 

                2702    2423     711
 
BsaBI GATnn'nnATC

Cuts at:   5830    5830 
   Size:       5836
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2154    2523    3643    4025 
   Size:         53      83     186     936     621     369    1120     382

Cuts at:   4025    4660     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451    1120     936     635     621     382     369     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   4269    4667    4941    5088    5499    4269 
   Size:        398     274     147     411    4606

  Fragments arranged by size: 

                4606     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2787    2817    1178 
   Size:         30     165      30    1384      30    4197

  Fragments arranged by size: 

                4197    1384     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3059    1186 
   Size:       1873    3963
 
BseYI C'CCAG_C

Cuts at:   2338    2638    2785    5039    5535    2338 
   Size:        300     147    2254     496    2639

  Fragments arranged by size: 

                2639    2254     496     300     147
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2239    2588    1163 
   Size:        573     503     349    4411

  Fragments arranged by size: 

                4411     573     503     349
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2078    3286    4047    5075    1540 
   Size:        275     186      77    1208     761    1028    2301

  Fragments arranged by size: 

                2301    1208    1028     761     275     186      77
 
BsiHKAI G_wGCw'C

Cuts at:   2601    3897    3982    5053    2601 
   Size:       1296      85    1071    3384

  Fragments arranged by size: 

                3384    1296    1071      85
 
BsiWI C'GTAC_G

Cuts at:   2167    2167 
   Size:       5836
 
BsmI GAATG_Cn'

Cuts at:   2194    3347    3440    2194 
   Size:       1153      93    4590

  Fragments arranged by size: 

                4590    1153      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2271    2601    3155    3897    3982    5053    1391 
   Size:        399     481     330     554     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     554     481     399     330      85
            
 
BspEI T'CCGG_A

Cuts at:   5499    5499 
   Size:       5836
 
BspHI T'CATG_A

Cuts at:   2376    3725    5455    2376 
   Size:       1349    1730    2757

  Fragments arranged by size: 

                2757    1730    1349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2541    3191     844 
   Size:       1697     650    3489

  Fragments arranged by size: 

                3489    1697     650
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3723    1394 
   Size:        429    1900    3507

  Fragments arranged by size: 

                3507    1900     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2820    4331    4505      65 
   Size:       1183    1572    1511     174    1396

  Fragments arranged by size: 

                1572    1511    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4480    4667    1700 
   Size:        110    2670     187    2869

  Fragments arranged by size: 

                2869    2670     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5836
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5836
 
BssSI C'ACGA_G

Cuts at:   3896    4908    3896 
   Size:       1012    4824
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2628     794 
   Size:       1834    4002
 
BstBI TT'CG_AA

Cuts at:   4640    4640 
   Size:       5836
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5836
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2491    2689    2491 
   Size:        198    5638
 
BstYI r'GATC_y

Cuts at:   1972    2743    2814    3061    3166    3533    3918    3935    5387 
   Size:        771      71     247     105     367     385      17    1452

Cuts at:   5387    5473    5485    5831    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     771     385     367     346     247     105
                  86      71      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3057    3057 
   Size:       5836
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2179     513 
   Size:        552     939     175    4170

  Fragments arranged by size: 

                4170     939     552     175
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2083    2230     487 
   Size:        683      56     857     147    4093

  Fragments arranged by size: 

                4093     857     683     147      56
 
BtsI GCAGTG_nn'

Cuts at:   2044    3078    3352    4146    4166    2044 
   Size:       1034     274     794      20    3714

  Fragments arranged by size: 

                3714    1034     794     274      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5836
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5801
 
DraI TTT'AAA

Cuts at:   3140    3140 
   Size:       5836
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4843     809 
   Size:        507    3527    1802

  Fragments arranged by size: 

                3527    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2075    2700    3009 
   Size:         54    1425      50     275      18     245     625     309

Cuts at:   3009    3283    4172    5801       8 
   Size:        274     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     625     309     275     274     245
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2075    3283    1537 
   Size:        275     263    1208    4090

  Fragments arranged by size: 

                4090    1208     275     263
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2432    2451    2654    3766    1353 
   Size:       1079      19     203    1112    3423

  Fragments arranged by size: 

                3423    1112    1079     203      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2658    4423    4797    4943     387 
   Size:       1064     138    1069    1765     374     146    1280

  Fragments arranged by size: 

                1765    1280    1069    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2599    2599 
   Size:       5836
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2178    2771    3140    3899    4496    5283    5551 
   Size:        393     546     593     369     759     597     787     268

Cuts at:   5551    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     787     759     597     593     546     393     369
                 268
 
EcoO109I rG'GnC_Cy

Cuts at:   2488    2488 
   Size:       5836
 
EcoRI G'AATT_C

Cuts at:   3145    3523    3145 
   Size:        378    5458
 
FspI TGC'GCA

Cuts at:   4342    4342 
   Size:       5836
 
HaeII r_GCGC'y

Cuts at:   1678    2135    2157    3008    4983    1678 
   Size:        457      22     851    1975    2531

  Fragments arranged by size: 

                2531    1975     851     457      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2637    2669    2787    2819    4477 
   Size:         32     135      32    1065      32     118      32    1658

Cuts at:   4477    4509    4551    4583    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1658    1065     135     118      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3180    3426     669 
   Size:        988     281    1242     246    3079

  Fragments arranged by size: 

                3079    1242     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5836
 
HpaI GTT'AAC

Cuts at:   3426    3426 
   Size:       5836
 
KasI G'GCGC_C

Cuts at:   2153    2153 
   Size:       5836
 
KpnI G_GTAC'C

Cuts at:   2533    3161    2533 
   Size:        628    5208
 
MfeI C'AATT_G

Cuts at:   3435    3435 
   Size:       5836
 
MluI A'CGCG_T

Cuts at:   5712    5712 
   Size:       5836
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4950    5134    1305 
   Size:       3645     184    2007

  Fragments arranged by size: 

                3645    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2702    3011      10 
   Size:         54    1425     343     870     309    2835

  Fragments arranged by size: 

                2835    1425     870     343     309      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2684    3794    4153    4312     518 
   Size:        648    1518    1110     359     159    2042

  Fragments arranged by size: 

                2042    1518    1110     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2181    2253    2422    3068    3252    3929    5077    5322 
   Size:        575      72     169     646     184     677    1148     245

Cuts at:   5322    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     646     575     245     184     169
                  72
 
NarI GG'CG_CC

Cuts at:   2154    2154 
   Size:       5836
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4345

  Fragments arranged by size: 

                4345     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5836
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5836
 
NotI GC'GGCC_GC

Cuts at:   2075    3283    2075 
   Size:       1208    4628
 
NspI r_CATG'y

Cuts at:   1132    1229    2203    2948    3194    4739    1132 
   Size:         97     974     745     246    1545    2229

  Fragments arranged by size: 

                2229    1545     974     745     246      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5836
 
PciI A'CATG_T

Cuts at:   4735    4735 
   Size:       5836
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5836
 
PmeI GTTT'AAAC

Cuts at:   3140    3140 
   Size:       5836
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3905    3937    5418    5450    3905 
   Size:         32    1481      32    4291

  Fragments arranged by size: 

                4291    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2488    2488 
   Size:       5836
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5836
 
PsiI TTA'TAA

Cuts at:   3406    3406 
   Size:       5836
 
PstI C_TGCA'G

Cuts at:    830    3188     830 
   Size:       2358    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5836
 
PvuII CAG'CTG

Cuts at:   2253    2422    2253 
   Size:        169    5667
 
SacI G_AGCT'C

Cuts at:   2601    2601 
   Size:       5836
 
SacII CC_GC'GG

Cuts at:   2182    2182 
   Size:       5836
 
SalI G'TCGA_C

Cuts at:   1936    3178    1936 
   Size:       1242    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2432    2432 
   Size:       5836
 
SbfI CC_TGCA'GG

Cuts at:   3188    3188 
   Size:       5836
 
ScaI AGT'ACT

Cuts at:   4084    5796    4084 
   Size:       1712    4124
 
SexAI A'CCwGG_T

Cuts at:   2490    2967    2490 
   Size:        477    5359
 
SfcI C'TryA_G

Cuts at:    826    1045    3184    4319    5000    5191     826 
   Size:        219    2139    1135     681     191    1471

  Fragments arranged by size: 

                2139    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2155    2155 
   Size:       5836
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5836
 
SmaI CCC'GGG

Cuts at:   1872    3163    1872 
   Size:       1291    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3940    4841    5103     820 
   Size:        197     926    1997     901     262    1553

  Fragments arranged by size: 

                1997    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3627     493 
   Size:       3134    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5836
 
SphI G_CATG'C

Cuts at:   1132    2948    3194    1132 
   Size:       1816     246    3774

  Fragments arranged by size: 

                3774    1816     246
 
SspI AAT'ATT

Cuts at:      5      52    2289    3760       5 
   Size:         47    2237    1471    2081

  Fragments arranged by size: 

                2237    2081    1471      47
 
StuI AGG'CCT

Cuts at:   1517    2760    1517 
   Size:       1243    4593
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2484    3012    3275     513 
   Size:        552     939     480     528     263    3074

  Fragments arranged by size: 

                3074     939     552     528     480     263
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3863    3880    4033    4218    4663     325 
   Size:       1255    2283      17     153     185     445    1498

  Fragments arranged by size: 

                2283    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2301    4082    5794 
   Size:        274      80      33      51    1443     324    1781    1712

Cuts at:   5794      96 
   Size:        138

  Fragments arranged by size: 

                1781    1712    1443     324     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2480    2512    5796    5828    2480 
   Size:         32    3284      32    2488

  Fragments arranged by size: 

                3284    2488      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5836
 
XbaI T'CTAG_A

Cuts at:   3172    3172 
   Size:       5836
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5836
 
XmaI C'CCGG_G

Cuts at:   1870    3161    1870 
   Size:       1291    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3965    3965 
   Size:       5836
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3644     276 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BfrBI
    BsmBI  BstZ17I   DraIII    EcoNI    EcoRV     FalI     FseI    FspAI
     NaeI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII    SanDI     SfiI     SrfI     SwaI     XcmI
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