(Circular) (Six-base) MAPSORT of: pFN21AE6351.seq Check: 5709 from: 1 to: 5836 pFN21AE6351 5836 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 2529 3157 2529 Size: 628 5208 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2178 3899 5283 5551 1632 Size: 546 1721 1384 268 1917 Fragments arranged by size: 1917 1721 1384 546 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 2684 1166 Size: 1518 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2138 2568 1184 Size: 954 430 4452 Fragments arranged by size: 4452 954 430 AlwNI CAG_nnn'CTG Cuts at: 2746 3579 5151 2746 Size: 833 1572 3431 Fragments arranged by size: 3431 1572 833 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2474 3145 3371 3523 5632 1493 Size: 981 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 981 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3161 1870 Size: 73 147 1071 4545 Fragments arranged by size: 4545 1071 147 73 BamHI G'GATC_C Cuts at: 3061 3166 3533 3061 Size: 105 367 5364 Fragments arranged by size: 5364 367 105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2529 2678 2716 3157 Size: 325 217 627 366 376 149 38 441 Cuts at: 3157 4612 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 441 376 366 325 217 149 38 BanII G_rGCy'C Cuts at: 1391 2271 2601 3155 1391 Size: 880 330 554 4072 Fragments arranged by size: 4072 880 554 330 BbeI G_GCGC'C Cuts at: 2157 2157 Size: 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2656 928 Size: 971 757 4108 Fragments arranged by size: 4108 971 757 BbvCI CC'TCA_GC Cuts at: 2018 2537 2018 Size: 519 5317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3090 3124 4027 4061 1903 Size: 34 1153 34 903 34 3678 Fragments arranged by size: 3678 1153 903 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2949 3253 2949 Size: 304 5532 Bme1580I G_kGCm'C Cuts at: 1790 3897 5053 1790 Size: 2107 1156 2573 Fragments arranged by size: 2573 2107 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2615 2615 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 3140 4496 1239 Size: 1532 369 1356 2579 Fragments arranged by size: 2579 1532 1356 369 Bpu10I CC'TnA_GC Cuts at: 2018 2507 2537 2018 Size: 489 30 5317 Fragments arranged by size: 5317 489 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 2523 3643 4025 Size: 53 83 186 936 621 369 1120 382 Cuts at: 4025 4660 275 Size: 635 1451 Fragments arranged by size: 1451 1120 936 635 621 382 369 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2787 2817 1178 Size: 30 165 30 1384 30 4197 Fragments arranged by size: 4197 1384 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3059 1186 Size: 1873 3963 BseYI C'CCAG_C Cuts at: 2338 2638 2785 5039 5535 2338 Size: 300 147 2254 496 2639 Fragments arranged by size: 2639 2254 496 300 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2239 2588 1163 Size: 573 503 349 4411 Fragments arranged by size: 4411 573 503 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 3286 4047 5075 1540 Size: 275 186 77 1208 761 1028 2301 Fragments arranged by size: 2301 1208 1028 761 275 186 77 BsiHKAI G_wGCw'C Cuts at: 2601 3897 3982 5053 2601 Size: 1296 85 1071 3384 Fragments arranged by size: 3384 1296 1071 85 BsiWI C'GTAC_G Cuts at: 2167 2167 Size: 5836 BsmI GAATG_Cn' Cuts at: 2194 3347 3440 2194 Size: 1153 93 4590 Fragments arranged by size: 4590 1153 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2601 3155 3897 3982 5053 1391 Size: 399 481 330 554 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 481 399 330 85 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 2376 3725 5455 2376 Size: 1349 1730 2757 Fragments arranged by size: 2757 1730 1349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3191 844 Size: 1697 650 3489 Fragments arranged by size: 3489 1697 650 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2820 4331 4505 65 Size: 1183 1572 1511 174 1396 Fragments arranged by size: 1572 1511 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2628 794 Size: 1834 4002 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2689 2491 Size: 198 5638 BstYI r'GATC_y Cuts at: 1972 2743 2814 3061 3166 3533 3918 3935 5387 Size: 771 71 247 105 367 385 17 1452 Cuts at: 5387 5473 5485 5831 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 771 385 367 346 247 105 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3057 3057 Size: 5836 BtgI C'CryG_G Cuts at: 513 1065 2004 2179 513 Size: 552 939 175 4170 Fragments arranged by size: 4170 939 552 175 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2083 2230 487 Size: 683 56 857 147 4093 Fragments arranged by size: 4093 857 683 147 56 BtsI GCAGTG_nn' Cuts at: 2044 3078 3352 4146 4166 2044 Size: 1034 274 794 20 3714 Fragments arranged by size: 3714 1034 794 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2700 3009 Size: 54 1425 50 275 18 245 625 309 Cuts at: 3009 3283 4172 5801 8 Size: 274 889 1629 43 Fragments arranged by size: 1629 1425 889 625 309 275 274 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 3283 1537 Size: 275 263 1208 4090 Fragments arranged by size: 4090 1208 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 2451 2654 3766 1353 Size: 1079 19 203 1112 3423 Fragments arranged by size: 3423 1112 1079 203 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2658 4423 4797 4943 387 Size: 1064 138 1069 1765 374 146 1280 Fragments arranged by size: 1765 1280 1069 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2599 2599 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2771 3140 3899 4496 5283 5551 Size: 393 546 593 369 759 597 787 268 Cuts at: 5551 1239 Size: 1524 Fragments arranged by size: 1524 787 759 597 593 546 393 369 268 EcoO109I rG'GnC_Cy Cuts at: 2488 2488 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2135 2157 3008 4983 1678 Size: 457 22 851 1975 2531 Fragments arranged by size: 2531 1975 851 457 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2637 2669 2787 2819 4477 Size: 32 135 32 1065 32 118 32 1658 Cuts at: 4477 4509 4551 4583 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1658 1065 135 118 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2153 2153 Size: 5836 KpnI G_GTAC'C Cuts at: 2533 3161 2533 Size: 628 5208 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 3011 10 Size: 54 1425 343 870 309 2835 Fragments arranged by size: 2835 1425 870 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 3794 4153 4312 518 Size: 648 1518 1110 359 159 2042 Fragments arranged by size: 2042 1518 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 2253 2422 3068 3252 3929 5077 5322 Size: 575 72 169 646 184 677 1148 245 Cuts at: 5322 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 646 575 245 184 169 72 NarI GG'CG_CC Cuts at: 2154 2154 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 2075 3283 2075 Size: 1208 4628 NspI r_CATG'y Cuts at: 1132 1229 2203 2948 3194 4739 1132 Size: 97 974 745 246 1545 2229 Fragments arranged by size: 2229 1545 974 745 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2253 2422 2253 Size: 169 5667 SacI G_AGCT'C Cuts at: 2601 2601 Size: 5836 SacII CC_GC'GG Cuts at: 2182 2182 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2432 2432 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SexAI A'CCwGG_T Cuts at: 2490 2967 2490 Size: 477 5359 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2155 2155 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 2948 3194 1132 Size: 1816 246 3774 Fragments arranged by size: 3774 1816 246 SspI AAT'ATT Cuts at: 5 52 2289 3760 5 Size: 47 2237 1471 2081 Fragments arranged by size: 2237 2081 1471 47 StuI AGG'CCT Cuts at: 1517 2760 1517 Size: 1243 4593 StyI C'CwwG_G Cuts at: 513 1065 2004 2484 3012 3275 513 Size: 552 939 480 528 263 3074 Fragments arranged by size: 3074 939 552 528 480 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4082 5794 Size: 274 80 33 51 1443 324 1781 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 1781 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 5796 5828 2480 Size: 32 3284 32 2488 Fragments arranged by size: 3284 2488 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI