(Circular) (Six-base) MAPSORT of: pFN21AE1353.seq Check: 4978 from: 1 to: 5326 pFN21AE1353 5326 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3136 278 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2129 2669 1937 Size: 192 540 4594 Fragments arranged by size: 4594 540 192 Acc65I G'GTAC_C Cuts at: 2319 2647 2319 Size: 328 4998 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3389 4773 5041 1632 Size: 1757 1384 268 1917 Fragments arranged by size: 1917 1757 1384 268 AfeI AGC'GCT Cuts at: 2234 2234 Size: 5326 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 4225 5202 1203 Size: 3022 977 1327 Fragments arranged by size: 3022 1327 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 4055 4055 Size: 5326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5326 AlwNI CAG_nnn'CTG Cuts at: 3069 4641 3069 Size: 1572 3754 ApaLI G'TGCA_C Cuts at: 3383 4539 3383 Size: 1156 4170 ApoI r'AATT_y Cuts at: 1493 2635 2861 3013 5122 1493 Size: 1142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 226 152 AseI AT'TA_AT Cuts at: 160 1032 3880 160 Size: 872 2848 1606 Fragments arranged by size: 2848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2323 2651 1870 Size: 73 208 172 328 4545 Fragments arranged by size: 4545 328 208 172 73 BamHI G'GATC_C Cuts at: 2656 3023 2656 Size: 367 4959 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2164 2189 2209 2319 2647 Size: 325 217 627 377 25 20 110 328 Cuts at: 2647 4102 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 377 328 325 217 110 25 20 BanII G_rGCy'C Cuts at: 1391 2012 2184 2645 1391 Size: 621 172 461 4072 Fragments arranged by size: 4072 621 461 172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 1903 Size: 34 1580 34 3678 Fragments arranged by size: 3678 1580 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3218 4428 3218 Size: 1210 4116 BclI T'GATC_A Cuts at: 1548 1548 Size: 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2281 3937 136 Size: 107 122 71 631 605 609 1656 1525 Fragments arranged by size: 1656 1525 631 609 605 122 107 71 BglII A'GATC_T Cuts at: 5321 5321 Size: 5326 BlpI GC'TnA_GC Cuts at: 2743 2743 Size: 5326 Bme1580I G_kGCm'C Cuts at: 1790 2169 2192 2214 2277 3387 4543 1790 Size: 379 23 22 63 1110 1156 2573 Fragments arranged by size: 2573 1156 1110 379 63 23 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2289 3057 4015 Size: 416 53 83 399 24 872 768 958 Cuts at: 4015 442 Size: 1753 Fragments arranged by size: 1753 958 872 768 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2496 3986 1239 Size: 1046 211 1490 2579 Fragments arranged by size: 2579 1490 1046 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 882 Size: 617 2490 2219 Fragments arranged by size: 2490 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3117 493 Size: 711 1913 2702 Fragments arranged by size: 2702 1913 711 BsaBI GATnn'nnATC Cuts at: 2303 5320 2303 Size: 3017 2309 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 3133 3515 4150 Size: 53 83 186 936 1059 541 382 635 Cuts at: 4150 275 Size: 1451 Fragments arranged by size: 1451 1059 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2295 2316 3759 4157 4431 4578 4989 2295 Size: 21 1443 398 274 147 411 2632 Fragments arranged by size: 2632 1443 411 398 274 147 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5101 Fragments arranged by size: 5101 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5326 BseYI C'CCAG_C Cuts at: 2076 2213 2335 2439 2460 2488 2543 4529 5025 Size: 137 122 104 21 28 55 1986 496 Cuts at: 5025 2076 Size: 2377 Fragments arranged by size: 2377 1986 496 137 122 104 55 28 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2249 2523 1163 Size: 573 513 274 3966 Fragments arranged by size: 3966 573 513 274 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2776 3537 4565 1540 Size: 275 186 775 761 1028 2301 Fragments arranged by size: 2301 1028 775 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2482 3387 3472 4543 2184 Size: 298 905 85 1071 2967 Fragments arranged by size: 2967 1071 905 298 85 BsmI GAATG_Cn' Cuts at: 2378 2837 2930 2378 Size: 459 93 4774 Fragments arranged by size: 4774 459 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2169 2184 2192 2214 2277 2482 Size: 399 222 157 15 8 22 63 205 Cuts at: 2482 2645 3387 3472 4543 1391 Size: 163 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 222 205 163 157 85 63 22 15 8 BspEI T'CCGG_A Cuts at: 2295 4989 2295 Size: 2694 2632 BspHI T'CATG_A Cuts at: 2310 3215 4945 2310 Size: 905 1730 2691 Fragments arranged by size: 2691 1730 905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 2581 2681 844 Size: 1306 431 100 3489 Fragments arranged by size: 3489 1306 431 100 BsrBI CCG'CTC Cuts at: 1394 1823 3213 1394 Size: 429 1390 3507 Fragments arranged by size: 3507 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3821 3995 65 Size: 1183 2573 174 1396 Fragments arranged by size: 2573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3970 4157 1700 Size: 110 2160 187 2869 Fragments arranged by size: 2869 2160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 3386 4398 3386 Size: 1012 4314 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5326 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2206 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2304 2656 3023 3408 3425 4877 4963 4975 Size: 332 352 367 385 17 1452 86 12 Cuts at: 4975 5321 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 352 346 332 86 17 12 BstZ17I GTA'TAC Cuts at: 2130 2130 Size: 5326 BtgI C'CryG_G Cuts at: 513 1065 2193 513 Size: 552 1128 3646 Fragments arranged by size: 3646 1128 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3636 3656 2842 Size: 794 20 4512 Fragments arranged by size: 4512 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2630 2630 Size: 5326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2196 2773 Size: 54 1425 50 275 18 262 104 577 Cuts at: 2773 3662 5291 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 577 275 262 104 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2773 1537 Size: 275 961 4090 Fragments arranged by size: 4090 961 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 1353 Size: 1903 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2009 3913 4287 4433 387 Size: 1064 138 420 1904 374 146 1280 Fragments arranged by size: 1904 1280 1064 420 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2182 Size: 5326 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2496 3389 3986 4773 5041 1239 Size: 393 653 211 893 597 787 268 1524 Fragments arranged by size: 1524 893 787 653 597 393 268 211 EcoO109I rG'GnC_Cy Cuts at: 2065 2522 2065 Size: 457 4869 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FspI TGC'GCA Cuts at: 3832 3832 Size: 5326 HaeII r_GCGC'y Cuts at: 1678 2236 2559 4473 1678 Size: 558 323 1914 2531 Fragments arranged by size: 2531 1914 558 323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373 Size: 32 135 32 2395 32 42 32 2626 Fragments arranged by size: 2626 2395 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 2916 669 Size: 988 281 732 246 3079 Fragments arranged by size: 3079 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KpnI G_GTAC'C Cuts at: 2323 2651 2323 Size: 328 4998 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4440 4624 1305 Size: 3135 184 2007 Fragments arranged by size: 3135 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2094 2198 10 Size: 54 1425 343 262 104 3138 Fragments arranged by size: 3138 1425 343 262 104 54 MslI CAynn'nnrTG Cuts at: 518 1166 3284 3643 3802 518 Size: 648 2118 359 159 2042 Fragments arranged by size: 2118 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2206 2217 2335 2492 2579 2585 2742 Size: 444 156 11 118 157 87 6 157 Cuts at: 2742 3419 4567 4812 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 245 157 157 156 118 87 11 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4774 NdeI CA'TA_TG Cuts at: 387 387 Size: 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2684 4229 1132 Size: 97 1455 1545 2229 Fragments arranged by size: 2229 1545 1455 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5326 PciI A'CATG_T Cuts at: 4225 4225 Size: 5326 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5326 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PstI C_TGCA'G Cuts at: 830 2049 2678 830 Size: 1219 629 3478 Fragments arranged by size: 3478 1219 629 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 PvuII CAG'CTG Cuts at: 2050 2206 2217 2335 2492 2050 Size: 156 11 118 157 4884 Fragments arranged by size: 4884 157 156 118 11 SacI G_AGCT'C Cuts at: 2184 2184 Size: 5326 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2045 2674 3809 4490 4681 826 Size: 219 1000 629 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 681 629 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2153 2653 1872 Size: 281 500 4545 Fragments arranged by size: 4545 500 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 3250 5 Size: 47 3198 2081 Fragments arranged by size: 3198 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5326 StyI C'CwwG_G Cuts at: 513 1065 2613 2765 513 Size: 552 1548 152 3074 Fragments arranged by size: 3074 1548 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2516 2557 3353 3370 3523 3708 4153 Size: 1255 936 41 796 17 153 185 445 Cuts at: 4153 325 Size: 1498 Fragments arranged by size: 1498 1255 936 796 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3572 5284 96 Size: 274 80 33 51 1443 1595 1712 138 Fragments arranged by size: 1712 1595 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5286 5318 5286 Size: 32 5294 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5326 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2151 2651 1870 Size: 281 500 4545 Fragments arranged by size: 4545 500 281 XmnI GAAnn'nnTTC Cuts at: 3455 3455 Size: 5326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3134 276 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI