(Linear) (Six-base) MAPSORT of: KIEE1353.seq Check: 2293 from: 1 to: 621 KIEE1353.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AccI GT'mk_AC Cuts at: 0 124 621 Size: 124 497 Acc65I G'GTAC_C Cuts at: 0 314 621 Size: 314 307 AfeI AGC'GCT Cuts at: 0 229 621 Size: 229 392 AvaI C'yCGr_G Cuts at: 0 146 318 621 Size: 146 172 303 Fragments arranged by size: 303 172 146 BanI G'GyrC_C Cuts at: 0 159 184 204 314 621 Size: 159 25 20 110 307 Fragments arranged by size: 307 159 110 25 20 BanII G_rGCy'C Cuts at: 0 7 179 621 Size: 7 172 442 Fragments arranged by size: 442 172 7 BglI GCCn_nnn'nGGC Cuts at: 0 276 621 Size: 276 345 Bme1580I G_kGCm'C Cuts at: 0 164 187 209 272 621 Size: 164 23 22 63 349 Fragments arranged by size: 349 164 63 23 22 BmrI ACTGGGnnnn_n' Cuts at: 0 284 621 Size: 284 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 491 621 Size: 280 211 130 Fragments arranged by size: 280 211 130 BsaBI GATnn'nnATC Cuts at: 0 298 621 Size: 298 323 BsaHI Gr'CG_yC Cuts at: 0 587 621 Size: 587 34 BsaWI w'CCGG_w Cuts at: 0 290 311 621 Size: 290 21 310 Fragments arranged by size: 310 290 21 BseYI C'CCAG_C Cuts at: 0 71 208 330 434 455 483 538 621 Size: 71 137 122 104 21 28 55 83 Fragments arranged by size: 137 122 104 83 71 55 28 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 518 621 Size: 244 274 103 Fragments arranged by size: 274 244 103 BsiHKAI G_wGCw'C Cuts at: 0 179 477 621 Size: 179 298 144 Fragments arranged by size: 298 179 144 BsmI GAATG_Cn' Cuts at: 0 373 621 Size: 373 248 Bsp1286I G_dGCh'C Cuts at: 0 7 164 179 187 209 272 477 621 Size: 7 157 15 8 22 63 205 144 Fragments arranged by size: 205 157 144 63 22 15 8 7 BspEI T'CCGG_A Cuts at: 0 290 621 Size: 290 331 BspHI T'CATG_A Cuts at: 0 305 621 Size: 305 316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 145 576 621 Size: 145 431 45 Fragments arranged by size: 431 145 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 201 621 Size: 201 420 BstYI r'GATC_y Cuts at: 0 299 621 Size: 299 322 BstZ17I GTA'TAC Cuts at: 0 125 621 Size: 125 496 BtgI C'CryG_G Cuts at: 0 188 621 Size: 188 433 EaeI y'GGCC_r Cuts at: 0 87 191 621 Size: 87 104 430 Fragments arranged by size: 430 104 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4 621 Size: 4 617 EcoICRI GAG'CTC Cuts at: 0 177 621 Size: 177 444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 491 621 Size: 280 211 130 Fragments arranged by size: 280 211 130 EcoO109I rG'GnC_Cy Cuts at: 0 60 517 621 Size: 60 457 104 Fragments arranged by size: 457 104 60 HaeII r_GCGC'y Cuts at: 0 231 554 621 Size: 231 323 67 Fragments arranged by size: 323 231 67 KpnI G_GTAC'C Cuts at: 0 318 621 Size: 318 303 MscI TGG'CCA Cuts at: 0 89 193 621 Size: 89 104 428 Fragments arranged by size: 428 104 89 MspA1I CmG'CkG Cuts at: 0 45 201 212 330 487 574 580 621 Size: 45 156 11 118 157 87 6 41 Fragments arranged by size: 157 156 118 87 45 41 11 6 PstI C_TGCA'G Cuts at: 0 44 621 Size: 44 577 PvuII CAG'CTG Cuts at: 0 45 201 212 330 487 621 Size: 45 156 11 118 157 134 Fragments arranged by size: 157 156 134 118 45 11 SacI G_AGCT'C Cuts at: 0 179 621 Size: 179 442 SfcI C'TryA_G Cuts at: 0 40 621 Size: 40 581 SmaI CCC'GGG Cuts at: 0 148 621 Size: 148 473 StyI C'CwwG_G Cuts at: 0 608 621 Size: 608 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 511 552 621 Size: 511 41 69 Fragments arranged by size: 511 69 41 XmaI C'CCGG_G Cuts at: 0 146 621 Size: 146 475 Enzymes that do cut: AccI Acc65I AfeI AvaI BanI BanII BglI Bme1580I BmrI BpmI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BstXI BstYI BstZ17I BtgI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII KpnI MscI MspA1I PstI PvuII SacI SfcI SmaI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BsiEI BsiWI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI