Restriction map for
pFN21AB4614
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 1646 ACATGT A^C[GA][CT]GT
AspHI 1225 GAGCAC G[AT]GC[AT]^C
AvaI 979 CCCGAG C^[CT]CG[GA]G
AvaII 1285 GGACC G^G[AT]CC
BanII 1388 GAGCCC G[GA]GC[CT]^C
BciVI 798 GGATAC GTATCC(6/5)
BlpI 1222 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1222 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 1225 GAGCAC G[AT]GC[AT]^C
BsoBI 979 CCCGAG C^[CT]CG[GA]G
Bsp1407I 217 TGTACA T^GTACA
BspLU11I 1646 ACATGT A^CATGT
BsrFI 273 GCCGGT [GA]^CCGG[CT]
BsrGI 217 TGTACA T^GTACA
BstAPI 250 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1017 GGTTACC G^GT[ACGT]ACC
BstPI 1017 GGTTACC G^GT[ACGT]ACC
CelII 1222 GCTGAGC GC^T[ACGT]AGC
Cfr10I 273 GCCGGT [GA]^CCGG[CT]
DraIII 724 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1093 CGGCCA [CT]^GGCC[GA]
EcoO65I 1017 GGTTACC G^GT[ACGT]ACC
EcoRI 1327 GAATTC G^AATTC
MspA1I 1037 CAGCTG C[AC]G^C[GT]G
NcoI 1614 CCATGG C^CATGG
NheI 657 GCTAGC G^CTAGC
PshAI 808 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1037 CAGCTG CAG^CTG
SexAI 1271 ACCAGGT A^CC[AT]GGT
SspBI 217 TGTACA T^GTACA
TaqI 1440 TCGA T^CGA
TthHB8I 1440 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 812 GGCGTC G[GA]^CG[CT]C
1066 GGCGTC
BamHI 679 GGATCC G^GATCC
1057 GGATCC
BbsI 805 GAAGAC GAAGAC(2/6)
405 GTCTTC
BfaI 92 CTAG C^TAG
658 CTAG
BpuAI 805 GAAGAC GAAGAC(2/6)
405 GTCTTC
BsaHI 812 GGCGTC G[GA]^CG[CT]C
1066 GGCGTC
BseRI 509 CTCCTC GAGGAG(10/8)
1378 GAGGAG
BsgI 257 CTGCAC GTGCAG(16/14)
575 CTGCAC
BstXI 31 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
861 CCAGATCACTGG
EarI 674 GAAGAG CTCTTC(1/4)
783 GAAGAG
HapII 48 CCGG C^CGG
274 CCGG
HgaI 813 GCGTC GACGC(5/10)
1067 GCGTC
Hin1I 812 GGCGTC G[GA]^CG[CT]C
1066 GGCGTC
HpaII 48 CCGG C^CGG
274 CCGG
Ksp632I 674 GAAGAG CTCTTC(1/4)
783 GAAGAG
MaeI 92 CTAG C^TAG
658 CTAG
MspI 48 CCGG C^CGG
274 CCGG
PflMI 817 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
997 CCAACCTCTGG
PleI 183 GACTC GAGTC(4/5)
237 GACTC
SphI 537 GCATGC GCATG^C
974 GCATGC
Van91I 817 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
997 CCAACCTCTGG
XcmI 356 CCACAAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1166 CCAACTCAGCTTTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
Aor51HI ApoI BanI BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI Eco47III EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII