################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AviII AvrII BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BpuAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 27 CGCG CG^CG AcyI 328 GGCGCC G[GA]^CG[CT]C AflIII 35 ACATGT A^C[GA][CT]GT AseI 808 ATTAAT AT^TAAT AsnI 808 ATTAAT AT^TAAT AspI 43 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 639 TGGCCA TGG^CCA BamHI 1144 GGATCC G^GATCC BbeI 328 GGCGCC GGCGC^C BcgI 561 CGAAGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 971 TGATCA T^GATCA BcnI 1064 CCCGG CC^[GC]GG BlpI 1084 GCTAAGC GC^T[ACGT]AGC BpmI 1352 CTGGAG CTGGAG(16/14) Bpu1102I 1084 GCTAAGC GC^T[ACGT]AGC BsaHI 328 GGCGCC G[GA]^CG[CT]C Bsp1407I 1553 TGTACA T^GTACA BspHI 1244 TCATGA T^CATGA BspLU11I 35 ACATGT A^CATGT BspMI 1481 ACCTGC ACCTGC(4/8) BsrBI 1323 GAGCGG CCGCTC(-3/-3) BsrDI 354 CATTGC GCAATG(2/0) BsrGI 1553 TGTACA T^GTACA BstEII 1395 GGTGACC G^GT[ACGT]ACC BstPI 1395 GGTGACC G^GT[ACGT]ACC BstUI 27 CGCG CG^CG CelII 1084 GCTAAGC GC^T[ACGT]AGC CfoI 329 GCGC GCG^C EaeI 639 TGGCCA [CT]^GGCC[GA] EarI 1177 GAAGAG CTCTTC(1/4) EcoO65I 1395 GGTGACC G^GT[ACGT]ACC EcoRV 1408 GATATC GAT^ATC EcoT14I 1007 CCTTGG C^C[AT][AT]GG FbaI 971 TGATCA T^GATCA HaeII 328 GGCGCC [GA]GCGC^[CT] HapII 1065 CCGG C^CGG HhaI 329 GCGC GCG^C Hin1I 328 GGCGCC G[GA]^CG[CT]C HinP1I 329 GCGC G^CGC HindIII 1154 AAGCTT A^AGCTT HpaII 1065 CCGG C^CGG KasI 328 GGCGCC G^GCGCC Ksp632I 1177 GAAGAG CTCTTC(1/4) MluNI 639 TGGCCA TGG^CCA MscI 639 TGGCCA TGG^CCA MslI 338 CACCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1065 CCGG C^CGG MvnI 27 CGCG CG^CG NarI 328 GGCGCC GG^CGCC NciI 1064 CCCGG CC^[GC]GG PflFI 43 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1313 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1248 GACTC GAGTC(4/5) PshBI 808 ATTAAT AT^TAAT RcaI 1244 TCATGA T^CATGA ScaI 1202 AGTACT AGT^ACT SfoI 328 GGCGCC GGC^GCC SspBI 1553 TGTACA T^GTACA StuI 935 AGGCCT AGG^CCT StyI 1007 CCTTGG C^C[AT][AT]GG Tth111I 43 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1313 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 353 CCATTGCATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 1304 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1529 CAGTAACTG AspHI 475 GAGCTC G[AT]GC[AT]^C 1123 GAGCTC AvaI 131 CTCGGG C^[CT]CG[GA]G 1387 CTCGGG AvaII 211 GGACC G^G[AT]CC 693 GGACC BanI 328 GGCGCC G^G[CT][GA]CC 607 GGCACC BanII 475 GAGCTC G[GA]GC[CT]^C 1123 GAGCTC BfaI 509 CTAG C^TAG 1367 CTAG BsaAI 184 TACGTA [CT]AC^GT[GA] 802 TACGTA BsaI 704 GAGACC GGTCTC(1/5) 1384 GGTCTC BseRI 697 CTCCTC GAGGAG(10/8) 1195 GAGGAG BsiHKAI 475 GAGCTC G[AT]GC[AT]^C 1123 GAGCTC BsmBI 286 CGTCTC CGTCTC(1/5) 769 GAGACG BsoBI 131 CTCGGG C^[CT]CG[GA]G 1387 CTCGGG BstXI 337 CCACCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1133 CCAAAGTGCTGG BstYI 664 GGATCT [GA]^GATC[CT] 1144 GGATCC DraIII 559 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1314 CACAAAGTG DsaI 1277 CCGTGG C^C[GA][CT]GG 1573 CCACGG HgaI 771 GACGC GACGC(5/10) 285 GCGTC HincII 739 GTTGAC GT[CT]^[GA]AC 1271 GTCAAC HindII 739 GTTGAC GT[CT]^[GA]AC 1271 GTCAAC MaeI 509 CTAG C^TAG 1367 CTAG MflI 664 GGATCT [GA]^GATC[CT] 1144 GGATCC MspA1I 886 CAGCGG C[AC]G^C[GT]G 1445 CAGCGG SacI 475 GAGCTC GAGCT^C 1123 GAGCTC SfaNI 421 GATGC GCATC(5/9) 988 GATGC SnaBI 184 TACGTA TAC^GTA 802 TACGTA TaqI 41 TCGA T^CGA 1077 TCGA TthHB8I 41 TCGA T^CGA 1077 TCGA XhoII 664 GGATCT [GA]^GATC[CT] 1144 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI