(Circular) (Six-base) MAPSORT of: pFN21AE4501.seq Check: 7867 from: 1 to: 6286 pFN21AE4501 6286 bp With 182 enzymes: SgfI * March 28, 2012 14:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2836 3530 4349 5733 6001 1632 Size: 1204 694 819 1384 268 1917 Fragments arranged by size: 1917 1384 1204 819 694 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2040 5185 6162 1203 Size: 837 3145 977 1327 Fragments arranged by size: 3145 1327 977 837 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AlwNI CAG_nnn'CTG Cuts at: 3314 3539 4029 5601 3314 Size: 225 490 1572 3999 Fragments arranged by size: 3999 1572 490 225 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 3595 3821 3973 6082 1493 Size: 2102 226 152 2109 1697 Fragments arranged by size: 2109 2102 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2814 4840 160 Size: 872 1782 2026 1606 Fragments arranged by size: 2026 1782 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 2136 3392 3611 1870 Size: 73 193 1256 219 4545 Fragments arranged by size: 4545 1256 219 193 73 BamHI G'GATC_C Cuts at: 3149 3616 3983 3149 Size: 467 367 5452 Fragments arranged by size: 5452 467 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2612 3607 5062 618 Size: 325 217 627 546 279 995 1455 1842 Fragments arranged by size: 1842 1455 995 627 546 325 279 217 BanII G_rGCy'C Cuts at: 1391 2484 3132 3605 1391 Size: 1093 648 473 4072 Fragments arranged by size: 4072 1093 648 473 BbeI G_GCGC'C Cuts at: 2337 2337 Size: 6286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2555 2589 4477 4511 1903 Size: 34 618 34 1888 34 3678 Fragments arranged by size: 3678 1888 618 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 2976 1548 Size: 1428 4858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3090 3703 3090 Size: 613 5673 Bme1580I G_kGCm'C Cuts at: 1790 2615 3308 4347 5503 1790 Size: 825 693 1039 1156 2573 Fragments arranged by size: 2573 1156 1039 825 693 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3226 3226 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3378 4946 1239 Size: 2139 1568 2579 Fragments arranged by size: 2579 2139 1568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2703 3395 4949 882 Size: 617 1204 692 1554 2219 Fragments arranged by size: 2219 1554 1204 692 617 BsaAI yAC'GTr Cuts at: 493 1204 2191 2809 4077 493 Size: 711 987 618 1268 2702 Fragments arranged by size: 2702 1268 987 711 618 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 4093 4475 5110 Size: 53 83 186 936 801 1759 382 635 Cuts at: 5110 275 Size: 1451 Fragments arranged by size: 1759 1451 936 801 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2196 2226 2700 2730 1178 Size: 30 165 30 793 30 474 30 4734 Fragments arranged by size: 4734 793 474 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2693 3215 1186 Size: 1507 522 4257 Fragments arranged by size: 4257 1507 522 BseYI C'CCAG_C Cuts at: 2254 3145 5489 5985 2254 Size: 891 2344 496 2555 Fragments arranged by size: 2555 2344 891 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5713 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2484 3132 4347 4432 5503 2484 Size: 648 1215 85 1071 3267 Fragments arranged by size: 3267 1215 1071 648 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 2768 2297 Size: 471 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 2615 3132 3308 3605 4347 4432 Size: 399 694 131 517 176 297 742 85 Cuts at: 4432 5503 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 694 517 399 297 176 131 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 3249 4175 5905 3249 Size: 926 1730 3630 Fragments arranged by size: 3630 1730 926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3495 3641 844 Size: 2651 146 3489 Fragments arranged by size: 3489 2651 146 BsrBI CCG'CTC Cuts at: 1394 1823 3330 4173 1394 Size: 429 1507 843 3507 Fragments arranged by size: 3507 1507 843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 4781 4955 65 Size: 1183 1110 2423 174 1396 Fragments arranged by size: 2423 1396 1183 1110 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3558 96 Size: 3462 2824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 3400 1184 Size: 2216 4070 BstXI CCAn_nnnn'nTGG Cuts at: 2349 3145 2349 Size: 796 5490 BstYI r'GATC_y Cuts at: 1972 2669 3149 3616 3983 4368 4385 5837 5923 Size: 697 480 467 367 385 17 1452 86 Cuts at: 5923 5935 6281 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 697 480 467 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3282 3578 513 Size: 552 939 1278 296 3221 Fragments arranged by size: 3221 1278 939 552 296 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 2609 3038 3802 4596 4616 2609 Size: 429 764 794 20 4279 Fragments arranged by size: 4279 794 764 429 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DraIII CAC_nnn'GTG Cuts at: 2569 3324 2569 Size: 755 5531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2644 3733 4622 Size: 54 1425 50 275 18 814 1089 889 Cuts at: 4622 6251 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1089 889 814 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3177 4216 1353 Size: 1824 1039 3423 Fragments arranged by size: 3423 1824 1039 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 3130 2482 Size: 648 5638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2836 3378 3530 4349 4946 5733 6001 Size: 393 1204 542 152 819 597 787 268 Cuts at: 6001 1239 Size: 1524 Fragments arranged by size: 1524 1204 819 787 597 542 393 268 152 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 EcoRV GAT'ATC Cuts at: 3415 3415 Size: 6286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2866 2959 2866 Size: 93 6193 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 2337 5433 1678 Size: 659 3096 2531 Fragments arranged by size: 3096 2531 659 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3238 3270 4927 4959 5001 Size: 32 135 32 1666 32 1657 32 42 Cuts at: 5001 5033 1373 Size: 32 2626 Fragments arranged by size: 2626 1666 1657 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2746 3278 3630 3876 669 Size: 988 281 808 532 352 246 3079 Fragments arranged by size: 3079 988 808 532 352 281 246 HindIII A'AGCT_T Cuts at: 748 3159 748 Size: 2411 3875 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KasI G'GCGC_C Cuts at: 2333 2333 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 2444 2852 5400 5584 1305 Size: 823 316 408 2548 184 2007 Fragments arranged by size: 2548 2007 823 408 316 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2646 10 Size: 54 1425 343 814 3650 Fragments arranged by size: 3650 1425 814 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2347 4244 4603 4762 518 Size: 648 1181 1897 359 159 2042 Fragments arranged by size: 2042 1897 1181 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2893 3452 3702 4379 5527 5772 1606 Size: 1287 559 250 677 1148 245 2120 Fragments arranged by size: 2120 1287 1148 677 559 250 245 NarI GG'CG_CC Cuts at: 2334 2334 Size: 6286 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4795 Fragments arranged by size: 4795 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2044 2183 2450 3644 5189 1132 Size: 97 815 139 267 1194 1545 2229 Fragments arranged by size: 2229 1545 1194 815 267 139 97 PasI CC'CwG_GG Cuts at: 1348 2433 3544 1348 Size: 1085 1111 4090 Fragments arranged by size: 4090 1111 1085 PciI A'CATG_T Cuts at: 2040 5185 2040 Size: 3145 3141 PflMI CCAn_nnn'nTGG Cuts at: 1108 3324 1108 Size: 2216 4070 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4355 4387 5868 5900 3476 Size: 32 847 32 1481 32 3862 Fragments arranged by size: 3862 1481 847 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 SacI G_AGCT'C Cuts at: 2484 3132 2484 Size: 648 5638 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 3209 4534 6246 3209 Size: 1325 1712 3249 Fragments arranged by size: 3249 1712 1325 SfcI C'TryA_G Cuts at: 826 1045 3634 4769 5450 5641 826 Size: 219 2589 1135 681 191 1471 Fragments arranged by size: 2589 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2335 2335 Size: 6286 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4390 5291 5553 820 Size: 197 926 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2191 2809 4077 493 Size: 1698 618 1268 2702 Fragments arranged by size: 2702 1698 1268 618 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 2942 1517 Size: 1425 4861 StyI C'CwwG_G Cuts at: 513 1065 2004 3012 3725 513 Size: 552 939 1008 713 3074 Fragments arranged by size: 3074 1008 939 713 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3419 3479 4313 4330 4483 4668 5113 Size: 1255 1839 60 834 17 153 185 445 Cuts at: 5113 325 Size: 1498 Fragments arranged by size: 1839 1498 1255 834 445 185 153 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3207 3558 4532 Size: 274 80 33 51 1443 1230 351 974 Cuts at: 4532 6244 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1230 974 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2700 2732 6246 6278 2196 Size: 32 472 32 3514 32 2204 Fragments arranged by size: 3514 2204 472 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2051 1316 Size: 735 5551 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2365 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI