################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI Ksp632I MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 462 GACGTC GACGT^C AcyI 462 GACGTC G[GA]^CG[CT]C Aor51HI 80 AGCGCT AGC^GCT Asp700I 948 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1150 GAGCAC G[AT]GC[AT]^C AvaI 167 CCCGGG C^[CT]CG[GA]G AvaII 40 GGACC G^G[AT]CC BalI 603 TGGCCA TGG^CCA BamHI 204 GGATCC G^GATCC BanI 545 GGCACC G^G[CT][GA]CC BbvCI 812 CCTCAGC CCTCAGC(-5/-2) BclI 1041 TGATCA T^GATCA BsaBI 1315 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 462 GACGTC G[GA]^CG[CT]C BsaI 25 GAGACC GGTCTC(1/5) BsaWI 1049 ACCGGA [AT]^CCGG[AT] BsiEI 114 CGACCG CG[GA][CT]^CG BsiHKAI 1150 GAGCAC G[AT]GC[AT]^C BsoBI 167 CCCGGG C^[CT]CG[GA]G Bsp1407I 125 TGTACA T^GTACA BspMI 404 ACCTGC ACCTGC(4/8) BsrDI 1091 GCAATG GCAATG(2/0) BsrFI 149 GCCGGC [GA]^CCGG[CT] BsrGI 125 TGTACA T^GTACA BstAPI 411 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 149 GCCGGC [GA]^CCGG[CT] DraIII 1189 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 80 AGCGCT AGC^GCT EcoT14I 719 CCTTGG C^C[AT][AT]GG EcoT22I 700 ATGCAT ATGCA^T FbaI 1041 TGATCA T^GATCA HaeII 80 AGCGCT [GA]GCGC^[CT] Hin1I 462 GACGTC G[GA]^CG[CT]C HinfI 1294 GAGTC G^A[ACGT]TC KspI 29 CCGCGG CCGC^GG MaeIII 1009 GTGAC ^GT[ACGT]AC MamI 1315 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 603 TGGCCA TGG^CCA MscI 603 TGGCCA TGG^CCA MspA1I 29 CCGCGG C[AC]G^C[GT]G NaeI 149 GCCGGC GCC^GGC NgoMIV 149 GCCGGC G^CCGGC NsiI 700 ATGCAT ATGCA^T PleI 1294 GAGTC GAGTC(4/5) PpuMI 39 GGGACCT [GA]G^G[AT]CC[CT] PstI 529 CTGCAG CTGCA^G SacII 29 CCGCGG CCGC^GG SfcI 529 CTGCAG C^T[GA][CT]AG SmaI 167 CCCGGG CCC^GGG SspBI 125 TGTACA T^GTACA StyI 719 CCTTGG C^C[AT][AT]GG Tsp45I 1009 GTGAC ^GT[GC]AC XmaCI 167 CCCGGG C^CCGGG XmaI 167 CCCGGG C^CCGGG XmnI 948 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 30 CGCG CG^CG 50 CGCG ApaI 617 GGGCCC GGGCC^C 808 GGGCCC BbsI 388 GTCTTC GAAGAC(2/6) 556 GTCTTC BfaI 106 CTAG C^TAG 1312 CTAG BpmI 281 CTCCAG CTGGAG(16/14) 1078 CTCCAG BpuAI 388 GTCTTC GAAGAC(2/6) 556 GTCTTC BseRI 481 CTCCTC GAGGAG(10/8) 94 GAGGAG BsmAI 25 GAGAC GTCTC(1/5) 279 GTCTC BspHI 371 TCATGA T^CATGA 1044 TCATGA BsrBI 97 GAGCGG CCGCTC(-3/-3) 358 GAGCGG BstUI 30 CGCG CG^CG 50 CGCG BstYI 204 GGATCC [GA]^GATC[CT] 728 AGATCC CfoI 51 GCGC GCG^C 81 GCGC DsaI 29 CCGCGG C^C[GA][CT]GG 200 CCACGG EaeI 17 TGGCCG [CT]^GGCC[GA] 603 TGGCCA HhaI 51 GCGC GCG^C 81 GCGC HinP1I 51 GCGC G^CGC 81 GCGC MaeI 106 CTAG C^TAG 1312 CTAG MaeII 463 ACGT A^CGT 908 ACGT MflI 204 GGATCC [GA]^GATC[CT] 728 AGATCC MslI 693 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 924 CACTTATGTG MvnI 30 CGCG CG^CG 50 CGCG PflMI 626 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1188 CCACAAAGTGG RcaI 371 TCATGA T^CATGA 1044 TCATGA TaqI 113 TCGA T^CGA 1176 TCGA Tsp509I 804 AATT ^AATT 1183 AATT TthHB8I 113 TCGA T^CGA 1176 TCGA Van91I 626 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1188 CCACAAAGTGG XhoII 204 GGATCC [GA]^GATC[CT] 728 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AlwI BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI