(Circular) (Six-base) MAPSORT of: pFN21AE4427.seq Check: 596 from: 1 to: 6037 pFN21AE4427 6037 bp With 182 enzymes: SgfI * March 28, 2012 14:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2471 3847 278 Size: 53 83 186 936 935 1376 2468 Fragments arranged by size: 2468 1376 936 935 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 3358 3358 Size: 6037 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2548 2636 4100 5484 5752 1632 Size: 916 88 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 916 268 88 AfeI AGC'GCT Cuts at: 2087 2087 Size: 6037 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 4936 5913 1203 Size: 3733 977 1327 Fragments arranged by size: 3733 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 2933 1166 Size: 1767 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AlwNI CAG_nnn'CTG Cuts at: 3780 5352 3780 Size: 1572 4465 ApaI G_GGCC'C Cuts at: 2626 2817 2626 Size: 191 5846 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 3346 3572 3724 5833 1493 Size: 1853 226 152 2109 1697 Fragments arranged by size: 2109 1853 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3362 1870 Size: 73 229 1190 4545 Fragments arranged by size: 4545 1190 229 73 BamHI G'GATC_C Cuts at: 2209 3367 3734 2209 Size: 1158 367 4512 Fragments arranged by size: 4512 1158 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2550 3358 4813 618 Size: 325 217 627 763 808 1455 1842 Fragments arranged by size: 1842 1455 808 763 627 325 217 BanII G_rGCy'C Cuts at: 1391 2171 2310 2376 2626 2817 3356 1391 Size: 780 139 66 250 191 539 4072 Fragments arranged by size: 4072 780 539 250 191 139 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 2554 928 Size: 971 487 168 4411 Fragments arranged by size: 4411 971 487 168 BbvCI CC'TCA_GC Cuts at: 2818 2818 Size: 6037 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 3046 1548 Size: 1498 4539 BfrBI ATG'CAT Cuts at: 2707 2707 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4648 136 Size: 107 122 71 631 605 2976 1525 Fragments arranged by size: 2976 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6032 6032 Size: 6037 BlpI GC'TnA_GC Cuts at: 3454 3454 Size: 6037 Bme1580I G_kGCm'C Cuts at: 1790 2553 2626 2817 4098 5254 1790 Size: 763 73 191 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 763 191 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3076 3768 4726 Size: 416 53 83 399 24 1659 692 958 Cuts at: 4726 442 Size: 1753 Fragments arranged by size: 1753 1659 958 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2271 3068 4697 1239 Size: 1032 797 1629 2579 Fragments arranged by size: 2579 1629 1032 797 Bpu10I CC'TnA_GC Cuts at: 2040 2089 2818 2040 Size: 49 729 5259 Fragments arranged by size: 5259 729 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4162 5027 5325 4162 Size: 865 298 4874 Fragments arranged by size: 4874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 4700 882 Size: 617 525 2676 2219 Fragments arranged by size: 2676 2219 617 525 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 3324 6031 3324 Size: 2707 3330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2468 3844 4226 4861 Size: 53 83 186 936 935 1376 382 635 Cuts at: 4861 275 Size: 1451 Fragments arranged by size: 1451 1376 936 935 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3054 4470 4868 5142 5289 5700 3054 Size: 1416 398 274 147 411 3391 Fragments arranged by size: 3391 1416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5812 Fragments arranged by size: 5812 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2477 1186 Size: 928 363 4746 Fragments arranged by size: 4746 928 363 BseYI C'CCAG_C Cuts at: 2082 2297 3078 5240 5736 2082 Size: 215 781 2162 496 2383 Fragments arranged by size: 2383 2162 781 496 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 2457 2529 3233 1163 Size: 573 663 58 72 704 3967 Fragments arranged by size: 3967 704 663 573 72 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 3487 4248 5276 1540 Size: 275 186 121 1365 761 1028 2301 Fragments arranged by size: 2301 1365 1028 761 275 186 121 BsiHKAI G_wGCw'C Cuts at: 3159 4098 4183 5254 3159 Size: 939 85 1071 3942 Fragments arranged by size: 3942 1071 939 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2171 2310 2376 2553 2626 2817 3159 Size: 399 381 139 66 177 73 191 342 Cuts at: 3159 3356 4098 4183 5254 1391 Size: 197 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 381 342 197 191 177 139 85 73 66 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 2376 3049 3926 5656 2376 Size: 673 877 1730 2757 Fragments arranged by size: 2757 1730 877 673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2418 3392 844 Size: 1574 974 3489 Fragments arranged by size: 3489 1574 974 BsrBI CCG'CTC Cuts at: 1394 1823 2104 2365 3924 1394 Size: 429 281 261 1559 3507 Fragments arranged by size: 3507 1559 429 281 261 BsrDI GCAATG_nn' Cuts at: 65 1248 3103 4532 4706 65 Size: 1183 1855 1429 174 1396 Fragments arranged by size: 1855 1429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2154 4681 4868 1700 Size: 110 344 2527 187 2869 Fragments arranged by size: 2869 2527 344 187 110 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 4003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 2422 794 Size: 1628 4409 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2209 2733 3367 3734 4119 4136 5588 5674 Size: 237 524 634 367 385 17 1452 86 Cuts at: 5674 5686 6032 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 634 524 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2205 513 Size: 552 939 30 171 4345 Fragments arranged by size: 4345 939 552 171 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5298 Fragments arranged by size: 5298 683 56 BtsI GCAGTG_nn' Cuts at: 3553 4347 4367 3553 Size: 794 20 5223 Fragments arranged by size: 5223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DraIII CAC_nnn'GTG Cuts at: 3199 3199 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2608 3484 Size: 54 1425 50 275 18 192 586 876 Cuts at: 3484 4373 6002 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 876 586 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3484 1537 Size: 275 1672 4090 Fragments arranged by size: 4090 1672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3967 1353 Size: 2614 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4624 4998 5144 387 Size: 1064 138 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2548 2636 3068 4100 4697 5484 Size: 393 639 277 88 432 1032 597 787 Cuts at: 5484 5752 1239 Size: 268 1524 Fragments arranged by size: 1524 1032 787 639 597 432 393 277 268 88 EcoO109I rG'GnC_Cy Cuts at: 2045 2239 2622 2623 2814 2045 Size: 194 383 1 191 5268 Fragments arranged by size: 5268 383 194 191 1 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3130 3130 Size: 6037 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 2089 5184 1678 Size: 411 3095 2531 Fragments arranged by size: 3095 2531 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2023 2055 3233 3265 4678 Size: 32 135 32 451 32 1178 32 1413 Cuts at: 4678 4710 4752 4784 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1413 1178 451 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3381 3627 669 Size: 988 281 1443 246 3079 Fragments arranged by size: 3079 1443 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 3362 3362 Size: 6037 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5151 5335 1305 Size: 3846 184 2007 Fragments arranged by size: 3846 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2610 10 Size: 54 1425 343 778 3437 Fragments arranged by size: 3437 1425 778 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 2933 3995 4354 4513 518 Size: 648 1536 231 1062 359 159 2042 Fragments arranged by size: 2042 1536 1062 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2036 3453 4130 5278 5523 1606 Size: 430 1417 677 1148 245 2120 Fragments arranged by size: 2120 1417 1148 677 430 245 NaeI GCC'GGC Cuts at: 2156 2156 Size: 6037 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4546 Fragments arranged by size: 4546 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NgoMIV G'CCGG_C Cuts at: 2154 2154 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NsiI A_TGCA'T Cuts at: 2709 2709 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2650 2707 3096 3395 4940 1132 Size: 97 1421 57 389 299 1545 2229 Fragments arranged by size: 2229 1545 1421 389 299 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 2637 3199 1108 Size: 1529 562 3946 Fragments arranged by size: 3946 1529 562 PfoI T'CCnGG_A Cuts at: 2340 2988 2340 Size: 648 5389 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4106 4138 5619 5651 4106 Size: 32 1481 32 4492 Fragments arranged by size: 4492 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2045 2045 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PspOMI G'GGCC_C Cuts at: 2622 2813 2622 Size: 191 5846 PstI C_TGCA'G Cuts at: 830 2538 3389 830 Size: 1708 851 3478 Fragments arranged by size: 3478 1708 851 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 SacII CC_GC'GG Cuts at: 2037 2037 Size: 6037 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SbfI CC_TGCA'GG Cuts at: 3389 3389 Size: 6037 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 2534 3385 4520 5201 5392 826 Size: 219 1489 851 1135 681 191 1471 Fragments arranged by size: 1489 1471 1135 851 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 2174 3364 1872 Size: 302 1190 4545 Fragments arranged by size: 4545 1190 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 4141 5042 5304 820 Size: 197 926 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6037 StyI C'CwwG_G Cuts at: 513 1065 2004 2724 3476 513 Size: 552 939 720 752 3074 Fragments arranged by size: 3074 939 752 720 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2548 2872 3001 3092 4064 4081 4234 Size: 1255 968 324 129 91 972 17 153 Cuts at: 4234 4419 4864 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 972 968 445 324 185 153 129 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 4283 5995 Size: 274 80 33 51 1443 153 2153 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 2153 1712 1443 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6037 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2172 3362 1870 Size: 302 1190 4545 Fragments arranged by size: 4545 1190 302 XmnI GAAnn'nnTTC Cuts at: 2957 4166 2957 Size: 1209 4828 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2469 3845 276 Size: 53 83 186 936 935 1376 2468 Fragments arranged by size: 2468 1376 936 935 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI