Restriction map for pFN21AE4427
 (Linear) (Six-base) MAPSORT of: KIEE4427.seq  Check: 9127  from: 1  to: 1332



KIEE4427.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:59  ..

AatII G_ACGT'C

Cuts at:      0     466    1332 
   Size:        466     866
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543     631    1332 
   Size:        543      88     701

  Fragments arranged by size: 

                 701     543      88
 
AfeI AGC'GCT

Cuts at:      0      82    1332 
   Size:         82    1250
 
AleI CACnn'nnGTG

Cuts at:      0     928    1332 
   Size:        928     404
 
ApaI G_GGCC'C

Cuts at:      0     621     812    1332 
   Size:        621     191     520

  Fragments arranged by size: 

                 621     520     191
 
AvaI C'yCGr_G

Cuts at:      0     167    1332 
   Size:        167    1165
 
BamHI G'GATC_C

Cuts at:      0     204    1332 
   Size:        204    1128
 
BanI G'GyrC_C

Cuts at:      0     545    1332 
   Size:        545     787
 
BanII G_rGCy'C

Cuts at:      0     166     305     371     621     812    1332 
   Size:        166     139      66     250     191     520

  Fragments arranged by size: 

                 520     250     191     166     139      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     381     549    1332 
   Size:        381     168     783

  Fragments arranged by size: 

                 783     381     168
 
BbvCI CC'TCA_GC

Cuts at:      0     813    1332 
   Size:        813     519
 
BclI T'GATC_A

Cuts at:      0    1041    1332 
   Size:       1041     291
 
BfrBI ATG'CAT

Cuts at:      0     702    1332 
   Size:        702     630
 
Bme1580I G_kGCm'C

Cuts at:      0     548     621     812    1332 
   Size:        548      73     191     520

  Fragments arranged by size: 

                 548     520     191      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1071    1332 
   Size:       1071     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266    1063    1332 
   Size:        266     797     269

  Fragments arranged by size: 

                 797     269     266
 
Bpu10I CC'TnA_GC

Cuts at:      0      35      84     813    1332 
   Size:         35      49     729     519

  Fragments arranged by size: 

                 729     519      49      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19    1332 
   Size:         19    1313
 
BsaBI GATnn'nnATC

Cuts at:      0    1319    1332 
   Size:       1319      13
 
BsaHI Gr'CG_yC

Cuts at:      0     463    1332 
   Size:        463     869
 
BsaWI w'CCGG_w

Cuts at:      0    1049    1332 
   Size:       1049     283
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     472    1332 
   Size:        109     363     860

  Fragments arranged by size: 

                 860     363     109
 
BseYI C'CCAG_C

Cuts at:      0      77     292    1073    1332 
   Size:         77     215     781     259

  Fragments arranged by size: 

                 781     259     215      77
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     452     524    1228    1332 
   Size:        394      58      72     704     104

  Fragments arranged by size: 

                 704     394     104      72      58
 
BsiEI CG_ry'CG

Cuts at:      0     117    1332 
   Size:        117    1215
 
BsiHKAI G_wGCw'C

Cuts at:      0    1154    1332 
   Size:       1154     178
 
Bsp1286I G_dGCh'C

Cuts at:      0     166     305     371     548     621     812    1154    1332 
   Size:        166     139      66     177      73     191     342     178

  Fragments arranged by size: 

                 342     191     178     177     166     139      73      66
            
 
BspHI T'CATG_A

Cuts at:      0     371    1044    1332 
   Size:        371     673     288

  Fragments arranged by size: 

                 673     371     288
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     413    1332 
   Size:        413     919
 
BsrBI CCG'CTC

Cuts at:      0      99     360    1332 
   Size:         99     261     972

  Fragments arranged by size: 

                 972     261      99
 
BsrDI GCAATG_nn'

Cuts at:      0    1098    1332 
   Size:       1098     234
 
BsrFI r'CCGG_y

Cuts at:      0     149    1332 
   Size:        149    1183
 
BsrGI T'GTAC_A

Cuts at:      0     125    1332 
   Size:        125    1207
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     417    1332 
   Size:        417     915
 
BstYI r'GATC_y

Cuts at:      0     204     728    1332 
   Size:        204     524     604

  Fragments arranged by size: 

                 604     524     204
 
BtgI C'CryG_G

Cuts at:      0      29     200    1332 
   Size:         29     171    1132

  Fragments arranged by size: 

                1132     171      29
 
DraIII CAC_nnn'GTG

Cuts at:      0    1194    1332 
   Size:       1194     138
 
EaeI y'GGCC_r

Cuts at:      0      17     603    1332 
   Size:         17     586     729

  Fragments arranged by size: 

                 729     586      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     543     631    1063    1332 
   Size:        266     277      88     432     269

  Fragments arranged by size: 

                 432     277     269     266      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0      40     234     617     618     809    1332 
   Size:         40     194     383       1     191     523

  Fragments arranged by size: 

                 523     383     194     191      40       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1125    1332 
   Size:       1125     207
 
HaeII r_GCGC'y

Cuts at:      0      84    1332 
   Size:         84    1248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      18      50    1228    1260    1332 
   Size:         18      32    1178      32      72

  Fragments arranged by size: 

                1178      72      32      32      18
 
MscI TGG'CCA

Cuts at:      0     605    1332 
   Size:        605     727
 
MslI CAynn'nnrTG

Cuts at:      0     697     928    1332 
   Size:        697     231     404

  Fragments arranged by size: 

                 697     404     231
 
MspA1I CmG'CkG

Cuts at:      0      31    1332 
   Size:         31    1301
 
NaeI GCC'GGC

Cuts at:      0     151    1332 
   Size:        151    1181
 
NgoMIV G'CCGG_C

Cuts at:      0     149    1332 
   Size:        149    1183
 
NsiI A_TGCA'T

Cuts at:      0     704    1332 
   Size:        704     628
 
NspI r_CATG'y

Cuts at:      0     645     702    1091    1332 
   Size:        645      57     389     241

  Fragments arranged by size: 

                 645     389     241      57
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     632    1194    1332 
   Size:        632     562     138

  Fragments arranged by size: 

                 632     562     138
 
PfoI T'CCnGG_A

Cuts at:      0     335     983    1332 
   Size:        335     648     349

  Fragments arranged by size: 

                 648     349     335
 
PpuMI rG'GwC_Cy

Cuts at:      0      40    1332 
   Size:         40    1292
 
PspOMI G'GGCC_C

Cuts at:      0     617     808    1332 
   Size:        617     191     524

  Fragments arranged by size: 

                 617     524     191
 
PstI C_TGCA'G

Cuts at:      0     533    1332 
   Size:        533     799
 
SacII CC_GC'GG

Cuts at:      0      32    1332 
   Size:         32    1300
 
SfcI C'TryA_G

Cuts at:      0     529    1332 
   Size:        529     803
 
SmaI CCC'GGG

Cuts at:      0     169    1332 
   Size:        169    1163
 
StyI C'CwwG_G

Cuts at:      0     719    1332 
   Size:        719     613
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     543     867     996    1087    1332 
   Size:        543     324     129      91     245

  Fragments arranged by size: 

                 543     324     245     129      91
 
TatI w'GTAC_w

Cuts at:      0     125    1332 
   Size:        125    1207
 
XmaI C'CCGG_G

Cuts at:      0     167    1332 
   Size:        167    1165
 
XmnI GAAnn'nnTTC

Cuts at:      0     952    1332 
   Size:        952     380
 
ZraI GAC'GTC

Cuts at:      0     464    1332 
   Size:        464     868
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AleI     ApaI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BclI    BfrBI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstYI     BtgI   DraIII     EaeI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI   NgoMIV
     NsiI     NspI    PflMI     PfoI    PpuMI   PspOMI     PstI    SacII
     SfcI     SmaI     StyI    TaqII     TatI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BcgI    BciVI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaXI    BsiWI     BsmI
    BsmBI    BspEI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI
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