(Linear) (Six-base) MAPSORT of: KIEE4427.seq Check: 9127 from: 1 to: 1332 KIEE4427.orf With 182 enzymes: SgfI * March 22, 2012 17:59 .. AatII G_ACGT'C Cuts at: 0 466 1332 Size: 466 866 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 543 631 1332 Size: 543 88 701 Fragments arranged by size: 701 543 88 AfeI AGC'GCT Cuts at: 0 82 1332 Size: 82 1250 AleI CACnn'nnGTG Cuts at: 0 928 1332 Size: 928 404 ApaI G_GGCC'C Cuts at: 0 621 812 1332 Size: 621 191 520 Fragments arranged by size: 621 520 191 AvaI C'yCGr_G Cuts at: 0 167 1332 Size: 167 1165 BamHI G'GATC_C Cuts at: 0 204 1332 Size: 204 1128 BanI G'GyrC_C Cuts at: 0 545 1332 Size: 545 787 BanII G_rGCy'C Cuts at: 0 166 305 371 621 812 1332 Size: 166 139 66 250 191 520 Fragments arranged by size: 520 250 191 166 139 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 381 549 1332 Size: 381 168 783 Fragments arranged by size: 783 381 168 BbvCI CC'TCA_GC Cuts at: 0 813 1332 Size: 813 519 BclI T'GATC_A Cuts at: 0 1041 1332 Size: 1041 291 BfrBI ATG'CAT Cuts at: 0 702 1332 Size: 702 630 Bme1580I G_kGCm'C Cuts at: 0 548 621 812 1332 Size: 548 73 191 520 Fragments arranged by size: 548 520 191 73 BmrI ACTGGGnnnn_n' Cuts at: 0 1071 1332 Size: 1071 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 1063 1332 Size: 266 797 269 Fragments arranged by size: 797 269 266 Bpu10I CC'TnA_GC Cuts at: 0 35 84 813 1332 Size: 35 49 729 519 Fragments arranged by size: 729 519 49 35 BsaI GGTCTCn'nnnn_ Cuts at: 0 19 1332 Size: 19 1313 BsaBI GATnn'nnATC Cuts at: 0 1319 1332 Size: 1319 13 BsaHI Gr'CG_yC Cuts at: 0 463 1332 Size: 463 869 BsaWI w'CCGG_w Cuts at: 0 1049 1332 Size: 1049 283 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 109 472 1332 Size: 109 363 860 Fragments arranged by size: 860 363 109 BseYI C'CCAG_C Cuts at: 0 77 292 1073 1332 Size: 77 215 781 259 Fragments arranged by size: 781 259 215 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 452 524 1228 1332 Size: 394 58 72 704 104 Fragments arranged by size: 704 394 104 72 58 BsiEI CG_ry'CG Cuts at: 0 117 1332 Size: 117 1215 BsiHKAI G_wGCw'C Cuts at: 0 1154 1332 Size: 1154 178 Bsp1286I G_dGCh'C Cuts at: 0 166 305 371 548 621 812 1154 1332 Size: 166 139 66 177 73 191 342 178 Fragments arranged by size: 342 191 178 177 166 139 73 66 BspHI T'CATG_A Cuts at: 0 371 1044 1332 Size: 371 673 288 Fragments arranged by size: 673 371 288 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 413 1332 Size: 413 919 BsrBI CCG'CTC Cuts at: 0 99 360 1332 Size: 99 261 972 Fragments arranged by size: 972 261 99 BsrDI GCAATG_nn' Cuts at: 0 1098 1332 Size: 1098 234 BsrFI r'CCGG_y Cuts at: 0 149 1332 Size: 149 1183 BsrGI T'GTAC_A Cuts at: 0 125 1332 Size: 125 1207 BstAPI GCAn_nnn'nTGC Cuts at: 0 417 1332 Size: 417 915 BstYI r'GATC_y Cuts at: 0 204 728 1332 Size: 204 524 604 Fragments arranged by size: 604 524 204 BtgI C'CryG_G Cuts at: 0 29 200 1332 Size: 29 171 1132 Fragments arranged by size: 1132 171 29 DraIII CAC_nnn'GTG Cuts at: 0 1194 1332 Size: 1194 138 EaeI y'GGCC_r Cuts at: 0 17 603 1332 Size: 17 586 729 Fragments arranged by size: 729 586 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 543 631 1063 1332 Size: 266 277 88 432 269 Fragments arranged by size: 432 277 269 266 88 EcoO109I rG'GnC_Cy Cuts at: 0 40 234 617 618 809 1332 Size: 40 194 383 1 191 523 Fragments arranged by size: 523 383 194 191 40 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1125 1332 Size: 1125 207 HaeII r_GCGC'y Cuts at: 0 84 1332 Size: 84 1248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 18 50 1228 1260 1332 Size: 18 32 1178 32 72 Fragments arranged by size: 1178 72 32 32 18 MscI TGG'CCA Cuts at: 0 605 1332 Size: 605 727 MslI CAynn'nnrTG Cuts at: 0 697 928 1332 Size: 697 231 404 Fragments arranged by size: 697 404 231 MspA1I CmG'CkG Cuts at: 0 31 1332 Size: 31 1301 NaeI GCC'GGC Cuts at: 0 151 1332 Size: 151 1181 NgoMIV G'CCGG_C Cuts at: 0 149 1332 Size: 149 1183 NsiI A_TGCA'T Cuts at: 0 704 1332 Size: 704 628 NspI r_CATG'y Cuts at: 0 645 702 1091 1332 Size: 645 57 389 241 Fragments arranged by size: 645 389 241 57 PflMI CCAn_nnn'nTGG Cuts at: 0 632 1194 1332 Size: 632 562 138 Fragments arranged by size: 632 562 138 PfoI T'CCnGG_A Cuts at: 0 335 983 1332 Size: 335 648 349 Fragments arranged by size: 648 349 335 PpuMI rG'GwC_Cy Cuts at: 0 40 1332 Size: 40 1292 PspOMI G'GGCC_C Cuts at: 0 617 808 1332 Size: 617 191 524 Fragments arranged by size: 617 524 191 PstI C_TGCA'G Cuts at: 0 533 1332 Size: 533 799 SacII CC_GC'GG Cuts at: 0 32 1332 Size: 32 1300 SfcI C'TryA_G Cuts at: 0 529 1332 Size: 529 803 SmaI CCC'GGG Cuts at: 0 169 1332 Size: 169 1163 StyI C'CwwG_G Cuts at: 0 719 1332 Size: 719 613 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 543 867 996 1087 1332 Size: 543 324 129 91 245 Fragments arranged by size: 543 324 245 129 91 TatI w'GTAC_w Cuts at: 0 125 1332 Size: 125 1207 XmaI C'CCGG_G Cuts at: 0 167 1332 Size: 167 1165 XmnI GAAnn'nnTTC Cuts at: 0 952 1332 Size: 952 380 ZraI GAC'GTC Cuts at: 0 464 1332 Size: 464 868 Enzymes that do cut: AatII AcuI AfeI AleI ApaI AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BtgI DraIII EaeI Eco57MI EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV NsiI NspI PflMI PfoI PpuMI PspOMI PstI SacII SfcI SmaI StyI TaqII TatI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaXI BsiWI BsmI BsmBI BspEI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI