(Circular) (Six-base) MAPSORT of: pFN21AB8203.seq Check: 837 from: 1 to: 5614 pFN21AB8203 5614 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2793 2793 Size: 5614 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 3677 5061 5329 1632 Size: 605 1440 1384 268 1917 Fragments arranged by size: 1917 1440 1384 605 268 AfeI AGC'GCT Cuts at: 2416 2441 2416 Size: 25 5589 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 4513 5490 1203 Size: 3310 977 1327 Fragments arranged by size: 3310 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 2278 4343 2278 Size: 2065 3549 AleI CACnn'nnGTG Cuts at: 1166 2559 1166 Size: 1393 4221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 2829 Size: 32 5582 AlwNI CAG_nnn'CTG Cuts at: 2242 2389 3357 4929 2242 Size: 147 968 1572 2927 Fragments arranged by size: 2927 1572 968 147 ApaI G_GGCC'C Cuts at: 2590 2590 Size: 5614 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2923 3149 3301 5410 1493 Size: 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 226 152 AscI GG'CGCG_CC Cuts at: 2257 2257 Size: 5614 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2074 2939 1870 Size: 73 120 11 865 4545 Fragments arranged by size: 4545 865 120 73 11 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 4390 618 Size: 325 217 627 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 2091 2590 2825 2933 1391 Size: 684 16 499 235 108 4072 Fragments arranged by size: 4072 684 499 235 108 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2087 2121 2755 2789 3805 3839 1903 Size: 34 150 34 634 34 1016 34 3678 Fragments arranged by size: 3678 1016 634 150 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2594 4225 136 Size: 107 122 71 631 605 922 1631 1525 Fragments arranged by size: 1631 1525 922 631 605 122 107 71 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2590 3675 4831 1790 Size: 800 1085 1156 2573 Fragments arranged by size: 2573 1156 1085 800 BmgBI CAC'GTC Cuts at: 1318 2581 1318 Size: 1263 4351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2739 3345 4303 Size: 416 53 83 399 24 1322 606 958 Cuts at: 4303 442 Size: 1753 Fragments arranged by size: 1753 1322 958 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2506 2506 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2339 2485 4274 1239 Size: 1046 54 146 1789 2579 Fragments arranged by size: 2579 1789 1046 146 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2075 3739 4604 4902 2075 Size: 1664 865 298 2787 Fragments arranged by size: 2787 1664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4277 882 Size: 617 2778 2219 Fragments arranged by size: 2778 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4047 4445 4719 4866 5277 4047 Size: 398 274 147 411 4384 Fragments arranged by size: 4384 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2471 2792 1186 Size: 847 438 321 4008 Fragments arranged by size: 4008 847 438 321 BseYI C'CCAG_C Cuts at: 2041 2411 2589 2898 4817 5313 2041 Size: 370 178 309 1919 496 2342 Fragments arranged by size: 2342 1919 496 370 309 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 1163 Size: 573 735 4306 Fragments arranged by size: 4306 735 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2155 2764 3064 3825 4853 1540 Size: 275 186 154 609 300 761 1028 2301 Fragments arranged by size: 2301 1028 761 609 300 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2091 2784 3675 3760 4831 2091 Size: 693 891 85 1071 2874 Fragments arranged by size: 2874 1071 891 693 85 BsmI GAATG_Cn' Cuts at: 2879 3125 3218 2879 Size: 246 93 5275 Fragments arranged by size: 5275 246 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2091 2590 2784 2825 2933 3675 Size: 399 285 16 499 194 41 108 742 Cuts at: 3675 3760 4831 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 399 285 194 108 85 41 16 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2793 2969 844 Size: 1949 176 3489 Fragments arranged by size: 3489 1949 176 BsrBI CCG'CTC Cuts at: 1394 1823 2461 3501 1394 Size: 429 638 1040 3507 Fragments arranged by size: 3507 1040 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2508 4109 4283 65 Size: 1183 1260 1601 174 1396 Fragments arranged by size: 1601 1396 1260 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2253 4258 4445 1700 Size: 110 443 2005 187 2869 Fragments arranged by size: 2869 2005 443 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 2079 2257 1925 Size: 154 178 5282 Fragments arranged by size: 5282 178 154 BssSI C'ACGA_G Cuts at: 2231 2555 3674 4686 2231 Size: 324 1119 1012 3159 Fragments arranged by size: 3159 1119 1012 324 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5614 BstBI TT'CG_AA Cuts at: 2422 4418 2422 Size: 1996 3618 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2944 3311 3696 3713 5165 5251 5263 5609 Size: 972 367 385 17 1452 86 12 346 Cuts at: 5609 1972 Size: 1977 Fragments arranged by size: 1977 1452 972 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2514 513 Size: 552 1449 3613 Fragments arranged by size: 3613 1449 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 3772 Fragments arranged by size: 3772 1103 683 56 BtsI GCAGTG_nn' Cuts at: 2315 3130 3924 3944 2315 Size: 815 794 20 3985 Fragments arranged by size: 3985 815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2579 4621 809 Size: 507 1263 2042 1802 Fragments arranged by size: 2042 1802 1263 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 2181 2244 Size: 54 1425 50 275 18 322 29 63 Cuts at: 2244 2373 3061 3950 5579 8 Size: 129 688 889 1629 43 Fragments arranged by size: 1629 1425 889 688 322 275 129 63 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2152 3061 1537 Size: 275 340 909 4090 Fragments arranged by size: 4090 909 340 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2424 2532 2660 3544 1353 Size: 710 361 108 128 884 3423 Fragments arranged by size: 3423 884 710 361 128 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2070 2645 4201 4575 4721 387 Size: 1064 138 481 575 1556 374 146 1280 Fragments arranged by size: 1556 1280 1064 575 481 374 146 138 EcoICRI GAG'CTC Cuts at: 2089 2089 Size: 5614 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2285 2339 2485 3677 4274 5061 Size: 393 605 48 54 146 1192 597 787 Cuts at: 5061 5329 1239 Size: 268 1524 Fragments arranged by size: 1524 1192 787 605 597 393 268 146 54 48 EcoNI CCTnn'n_nnAGG Cuts at: 2375 2831 2375 Size: 456 5158 EcoO109I rG'GnC_Cy Cuts at: 2239 2239 Size: 5614 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2728 2311 Size: 417 5197 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 2049 2418 2443 4761 1678 Size: 371 369 25 2318 2531 Fragments arranged by size: 2531 2318 371 369 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 4255 4287 4329 Size: 32 135 32 692 32 1959 32 42 Cuts at: 4329 4361 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 692 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3249 Fragments arranged by size: 3249 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 3572 3931 4090 518 Size: 648 1393 1013 359 159 2042 Fragments arranged by size: 2042 1393 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2317 2386 2516 3030 3707 4855 5100 Size: 570 141 69 130 514 677 1148 245 Cuts at: 5100 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 570 514 245 141 130 69 NaeI GCC'GGC Cuts at: 2255 2255 Size: 5614 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5062 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NgoMIV G'CCGG_C Cuts at: 2253 2253 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NruI TCG'CGA Cuts at: 2429 2429 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2972 4517 1132 Size: 97 1743 1545 2229 Fragments arranged by size: 2229 1743 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PspOMI G'GGCC_C Cuts at: 2586 2586 Size: 5614 PstI C_TGCA'G Cuts at: 830 2358 2790 2966 830 Size: 1528 432 176 3478 Fragments arranged by size: 3478 1528 432 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 PvuII CAG'CTG Cuts at: 2317 2317 Size: 5614 SacI G_AGCT'C Cuts at: 2091 2091 Size: 5614 SacII CC_GC'GG Cuts at: 2517 2517 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SapI GCTCTTCn'nnn_ Cuts at: 2063 2424 2063 Size: 361 5253 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2354 2786 2962 4097 4778 4969 826 Size: 219 1309 432 176 1135 681 191 1471 Fragments arranged by size: 1471 1309 1135 681 432 219 191 176 SfiI GGCCn_nnn'nGGCC Cuts at: 2594 2594 Size: 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 3718 4619 4881 820 Size: 197 926 147 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 147 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 4534 StyI C'CwwG_G Cuts at: 513 1065 2376 3053 513 Size: 552 1311 677 3074 Fragments arranged by size: 3074 1311 677 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2890 3641 3658 3811 3996 4441 325 Size: 1255 1310 751 17 153 185 445 1498 Fragments arranged by size: 1498 1310 1255 751 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 3860 5572 Size: 274 80 33 51 1443 645 1238 1712 Cuts at: 5572 96 Size: 138 Fragments arranged by size: 1712 1443 1238 645 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5574 5606 5574 Size: 32 5582 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI XcmI