Restriction map for
pFN21AB8203
(Linear) (Six-base) MAPSORT of: KIBB8203.seq Check: 2480 from: 1 to: 909
KIBB8203.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 788 909
Size: 788 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 909
Size: 232 677
AfeI AGC'GCT
Cuts at: 0 411 436 909
Size: 411 25 473
Fragments arranged by size:
473 411 25
AhdI GACnn_n'nnGTC
Cuts at: 0 273 909
Size: 273 636
AleI CACnn'nnGTG
Cuts at: 0 554 909
Size: 554 355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 909
Size: 824 32 53
Fragments arranged by size:
824 53 32
AlwNI CAG_nnn'CTG
Cuts at: 0 237 384 909
Size: 237 147 525
Fragments arranged by size:
525 237 147
ApaI G_GGCC'C
Cuts at: 0 585 909
Size: 585 324
AscI GG'CGCG_CC
Cuts at: 0 252 909
Size: 252 657
AvaI C'yCGr_G
Cuts at: 0 58 69 909
Size: 58 11 840
Fragments arranged by size:
840 58 11
BanII G_rGCy'C
Cuts at: 0 70 86 585 820 909
Size: 70 16 499 235 89
Fragments arranged by size:
499 235 89 70 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 82 116 750 784 909
Size: 82 34 634 34 125
Fragments arranged by size:
634 125 82 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 589 909
Size: 589 320
Bme1580I G_kGCm'C
Cuts at: 0 585 909
Size: 585 324
BmgBI CAC'GTC
Cuts at: 0 576 909
Size: 576 333
BmrI ACTGGGnnnn_n'
Cuts at: 0 734 909
Size: 734 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 501 909
Size: 501 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 334 480 909
Size: 280 54 146 429
Fragments arranged by size:
429 280 146 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 909
Size: 70 839
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 466 787 909
Size: 28 438 321 122
Fragments arranged by size:
438 321 122 28
BseYI C'CCAG_C
Cuts at: 0 36 406 584 893 909
Size: 36 370 178 309 16
Fragments arranged by size:
370 309 178 36 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 909
Size: 466 443
BsiEI CG_ry'CG
Cuts at: 0 150 759 909
Size: 150 609 150
Fragments arranged by size:
609 150 150
BsiHKAI G_wGCw'C
Cuts at: 0 86 779 909
Size: 86 693 130
Fragments arranged by size:
693 130 86
BsmI GAATG_Cn'
Cuts at: 0 874 909
Size: 874 35
Bsp1286I G_dGCh'C
Cuts at: 0 70 86 585 779 820 909
Size: 70 16 499 194 41 89
Fragments arranged by size:
499 194 89 70 41 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 788 909
Size: 788 121
BsrBI CCG'CTC
Cuts at: 0 456 909
Size: 456 453
BsrDI GCAATG_nn'
Cuts at: 0 503 909
Size: 503 406
BsrFI r'CCGG_y
Cuts at: 0 248 909
Size: 248 661
BssHII G'CGCG_C
Cuts at: 0 74 252 909
Size: 74 178 657
Fragments arranged by size:
657 178 74
BssSI C'ACGA_G
Cuts at: 0 226 550 909
Size: 226 324 359
Fragments arranged by size:
359 324 226
BstBI TT'CG_AA
Cuts at: 0 417 909
Size: 417 492
BtgI C'CryG_G
Cuts at: 0 509 909
Size: 509 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 909
Size: 324 585
BtsI GCAGTG_nn'
Cuts at: 0 310 909
Size: 310 599
DrdI GACnn_nn'nnGTC
Cuts at: 0 574 909
Size: 574 335
EaeI y'GGCC_r
Cuts at: 0 147 176 239 368 909
Size: 147 29 63 129 541
Fragments arranged by size:
541 147 129 63 29
EagI C'GGCC_G
Cuts at: 0 147 909
Size: 147 762
EarI CTCTTCn'nnn_
Cuts at: 0 58 419 527 655 909
Size: 58 361 108 128 254
Fragments arranged by size:
361 254 128 108 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 640 909
Size: 65 575 269
Fragments arranged by size:
575 269 65
EcoICRI GAG'CTC
Cuts at: 0 84 909
Size: 84 825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 280 334 480 909
Size: 232 48 54 146 429
Fragments arranged by size:
429 232 146 54 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 826 909
Size: 370 456 83
Fragments arranged by size:
456 370 83
EcoO109I rG'GnC_Cy
Cuts at: 0 234 909
Size: 234 675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 723 909
Size: 306 417 186
Fragments arranged by size:
417 306 186
HaeII r_GCGC'y
Cuts at: 0 44 413 438 909
Size: 44 369 25 471
Fragments arranged by size:
471 369 44 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 909
Size: 259 32 618
Fragments arranged by size:
618 259 32
MscI TGG'CCA
Cuts at: 0 370 909
Size: 370 539
MslI CAynn'nnrTG
Cuts at: 0 554 909
Size: 554 355
MspA1I CmG'CkG
Cuts at: 0 171 312 381 511 909
Size: 171 141 69 130 398
Fragments arranged by size:
398 171 141 130 69
NaeI GCC'GGC
Cuts at: 0 250 909
Size: 250 659
NgoMIV G'CCGG_C
Cuts at: 0 248 909
Size: 248 661
NruI TCG'CGA
Cuts at: 0 424 909
Size: 424 485
PspOMI G'GGCC_C
Cuts at: 0 581 909
Size: 581 328
PstI C_TGCA'G
Cuts at: 0 353 785 909
Size: 353 432 124
Fragments arranged by size:
432 353 124
PvuII CAG'CTG
Cuts at: 0 312 909
Size: 312 597
SacI G_AGCT'C
Cuts at: 0 86 909
Size: 86 823
SacII CC_GC'GG
Cuts at: 0 512 909
Size: 512 397
SapI GCTCTTCn'nnn_
Cuts at: 0 58 419 909
Size: 58 361 490
Fragments arranged by size:
490 361 58
SfcI C'TryA_G
Cuts at: 0 349 781 909
Size: 349 432 128
Fragments arranged by size:
432 349 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 589 909
Size: 589 320
SmlI C'TyrA_G
Cuts at: 0 85 909
Size: 85 824
StuI AGG'CCT
Cuts at: 0 592 909
Size: 592 317
StyI C'CwwG_G
Cuts at: 0 371 909
Size: 371 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 885 909
Size: 885 24
TatI w'GTAC_w
Cuts at: 0 617 909
Size: 617 292
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AloI AlwNI ApaI
AscI AvaI BanII BcgI BglI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MscI MslI MspA1I NaeI NgoMIV NruI PspOMI PstI
PvuII SacI SacII SapI SfcI SfiI SmlI StuI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AlfI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BspHI BsrGI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI