Restriction map for pFN21AE4314
 (Circular) (Six-base) MAPSORT of: pFN21AE4314.seq  Check: 9540  from: 1  to: 5674



pFN21AE4314  5674 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2839    2839 
   Size:       5674
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3484     278 
   Size:         53      83     186     936    1948    2468

  Fragments arranged by size: 

                2468    1948     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3017    1937 
   Size:       1080    4594
 
Acc65I G'GTAC_C

Cuts at:   2995    2995 
   Size:       5674
 
AclI AA'CG_TT

Cuts at:   1563    3801    4174    1563 
   Size:       2238     373    3063

  Fragments arranged by size: 

                3063    2238     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2856    3737    5121    5389    1632 
   Size:       1224     881    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1224     881     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5477
 
AflIII A'CryG_T

Cuts at:   1203    2161    4573    5550    1203 
   Size:        958    2412     977    1327

  Fragments arranged by size: 

                2412    1327     977     958
 
AgeI A'CCGG_T

Cuts at:   4505    4505 
   Size:       5674
 
AhdI GACnn_n'nnGTC

Cuts at:   4403    4403 
   Size:       5674
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5674
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5674
 
AlwNI CAG_nnn'CTG

Cuts at:   2366    3417    4989    2366 
   Size:       1051    1572    3051

  Fragments arranged by size: 

                3051    1572    1051
 
ApaLI G'TGCA_C

Cuts at:   3731    4887    3731 
   Size:       1156    4518
 
ApoI r'AATT_y

Cuts at:   1493    2858    2983    3209    3361    5470    1493 
   Size:       1365     125     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1365     226     152     125
 
AseI AT'TA_AT

Cuts at:    160    1032    4228     160 
   Size:        872    3196    1606

  Fragments arranged by size: 

                3196    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5674
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2999    1870 
   Size:         73    1056    4545

  Fragments arranged by size: 

                4545    1056      73
 
BamHI G'GATC_C

Cuts at:   2663    3004    3371    2663 
   Size:        341     367    4966

  Fragments arranged by size: 

                4966     367     341
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2069    2995    4450     618 
   Size:        325     217     627     282     926    1455    1842

  Fragments arranged by size: 

                1842    1455     926     627     325     282     217
 
BanII G_rGCy'C

Cuts at:   1391    2550    2993    1391 
   Size:       1159     443    4072

  Fragments arranged by size: 

                4072    1159     443
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2241     928 
   Size:        971     342    4361

  Fragments arranged by size: 

                4361     971     342
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3865    3899    1903 
   Size:         34    1928      34    3678

  Fragments arranged by size: 

                3678    1928      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3566    4776    3566 
   Size:       1210    4464
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5674
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4285     136 
   Size:        107     122      71     631     605    2613    1525

  Fragments arranged by size: 

                2613    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5669    5669 
   Size:       5674
 
BlpI GC'TnA_GC

Cuts at:   3091    3091 
   Size:       5674
 
Bme1580I G_kGCm'C

Cuts at:   1790    3735    4891    1790 
   Size:       1945    1156    2573

  Fragments arranged by size: 

                2573    1945    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5674
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2714    3405    4363 
   Size:        416      53      83     399      24    1297     691     958

Cuts at:   4363     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1297     958     691     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5674
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2543    4334    1239 
   Size:       1304    1791    2579

  Fragments arranged by size: 

                2579    1791    1304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2534    3799    4664    4962    2534 
   Size:       1265     865     298    3246

  Fragments arranged by size: 

                3246    1265     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4337     882 
   Size:        617    2838    2219

  Fragments arranged by size: 

                2838    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3465     493 
   Size:        711    2261    2702

  Fragments arranged by size: 

                2702    2261     711
 
BsaBI GATnn'nnATC

Cuts at:   5668    5668 
   Size:       5674
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3481    3863    4498     275 
   Size:         53      83     186     936    1948     382     635    1451

  Fragments arranged by size: 

                1948    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4107    4505    4779    4926    5337    4107 
   Size:        398     274     147     411    4444

  Fragments arranged by size: 

                4444     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2681    2711    1178 
   Size:         30     165      30    1278      30    4141

  Fragments arranged by size: 

                4141    1278     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2192    2891    1186 
   Size:       1006     699    3969

  Fragments arranged by size: 

                3969    1006     699
 
BseYI C'CCAG_C

Cuts at:   2758    4877    5373    2758 
   Size:       2119     496    3059

  Fragments arranged by size: 

                3059    2119     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2142    2867    1163 
   Size:        573     406     725    3970

  Fragments arranged by size: 

                3970     725     573     406
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3124    3885    4913    1540 
   Size:        275     186    1123     761    1028    2301

  Fragments arranged by size: 

                2301    1123    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2659    2709    3735    3820    4891    2659 
   Size:         50    1026      85    1071    3442

  Fragments arranged by size: 

                3442    1071    1026      85      50
 
BsmI GAATG_Cn'

Cuts at:   3185    3278    3185 
   Size:         93    5581
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2550    2659    2709    2993    3735    3820    4891 
   Size:        399     760     109      50     284     742      85    1071

Cuts at:   4891    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     760     742     399     284     109      85
                  50
 
BspEI T'CCGG_A

Cuts at:   5337    5337 
   Size:       5674
 
BspHI T'CATG_A

Cuts at:   3563    5293    3563 
   Size:       1730    3944
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2173    2839    3029     844 
   Size:       1329     666     190    3489

  Fragments arranged by size: 

                3489    1329     666     190
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3561    1394 
   Size:        429    1738    3507

  Fragments arranged by size: 

                3507    1738     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4169    4343      65 
   Size:       1183    2921     174    1396

  Fragments arranged by size: 

                2921    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4318    4505    1700 
   Size:        110    2508     187    2869

  Fragments arranged by size: 

                2869    2508     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5674
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5674
 
BssSI C'ACGA_G

Cuts at:   3734    4746    3734 
   Size:       1012    4662
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5674
 
BstBI TT'CG_AA

Cuts at:   2012    4478    2012 
   Size:       2466    3208
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5674
 
BstYI r'GATC_y

Cuts at:   1972    2431    2663    3004    3371    3756    3773    5225    5311 
   Size:        459     232     341     367     385      17    1452      86

Cuts at:   5311    5323    5669    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     459     385     367     346     341     232
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2454    2681     513 
   Size:        552     939     450     227    3506

  Fragments arranged by size: 

                3506     939     552     450     227
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4935

  Fragments arranged by size: 

                4935     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2227    3190    3984    4004    2227 
   Size:        963     794      20    3897

  Fragments arranged by size: 

                3897     963     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5674
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5639
 
DraI TTT'AAA

Cuts at:   2978    2978 
   Size:       5674
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4681     809 
   Size:        507    3365    1802

  Fragments arranged by size: 

                3365    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2193    2481    2808 
   Size:         54    1425      50     275      18     363     288     327

Cuts at:   2808    3121    4010    5639       8 
   Size:        313     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     363     327     313     288     275
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3121    1537 
   Size:        275    1309    4090

  Fragments arranged by size: 

                4090    1309     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2222    3604    1353 
   Size:        869    1382    3423

  Fragments arranged by size: 

                3423    1382     869
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4261    4635    4781     387 
   Size:       1064     138    2672     374     146    1280

  Fragments arranged by size: 

                2672    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2543    2856    3737    4334    5121    5389    1239 
   Size:        393     911     313     881     597     787     268    1524

  Fragments arranged by size: 

                1524     911     881     787     597     393     313     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2302    2302 
   Size:       5674
 
EcoO109I rG'GnC_Cy

Cuts at:   2716    2716 
   Size:       5674
 
EcoRI G'AATT_C

Cuts at:   2858    2983    3361    2858 
   Size:        125     378    5171

  Fragments arranged by size: 

                5171     378     125
 
FspI TGC'GCA

Cuts at:   4180    4180 
   Size:       5674
 
HaeII r_GCGC'y

Cuts at:   1678    4821    1678 
   Size:       3143    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2181    2213    2681    2713    4315 
   Size:         32     135      32     609      32     468      32    1602

Cuts at:   4315    4347    4389    4421    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1602     609     468     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2338    2868    3018    3264     669 
   Size:        988     281     400     530     150     246    3079

  Fragments arranged by size: 

                3079     988     530     400     281     246     150
 
HindIII A'AGCT_T

Cuts at:    748    2255     748 
   Size:       1507    4167
 
HpaI GTT'AAC

Cuts at:   3264    3264 
   Size:       5674
 
KpnI G_GTAC'C

Cuts at:   2999    2999 
   Size:       5674
 
MfeI C'AATT_G

Cuts at:   2324    3273    2324 
   Size:        949    4725
 
MluI A'CGCG_T

Cuts at:   5550    5550 
   Size:       5674
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2167    2314    2526    4788    4972    1305 
   Size:        862     147     212    2262     184    2007

  Fragments arranged by size: 

                2262    2007     862     212     184     147
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2195    2483    2810      10 
   Size:         54    1425     343     363     288     327    2874

  Fragments arranged by size: 

                2874    1425     363     343     327     288      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2495    3632    3991    4150     518 
   Size:        648    1329    1137     359     159    2042

  Fragments arranged by size: 

                2042    1329    1137     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2785    3090    3767    4915    5160    1606 
   Size:       1179     305     677    1148     245    2120

  Fragments arranged by size: 

                2120    1179    1148     677     305     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2454    2681     513 
   Size:        552     939     450     227    3506

  Fragments arranged by size: 

                3506     939     552     450     227
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5674
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5674
 
NotI GC'GGCC_GC

Cuts at:   3121    3121 
   Size:       5674
 
NspI r_CATG'y

Cuts at:   1132    1229    2165    3032    4577    1132 
   Size:         97     936     867    1545    2229

  Fragments arranged by size: 

                2229    1545     936     867      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5674
 
PciI A'CATG_T

Cuts at:   2161    4573    2161 
   Size:       2412    3262
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5674
 
PmeI GTTT'AAAC

Cuts at:   2978    2978 
   Size:       5674
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3743    3775    5256    5288    3743 
   Size:         32    1481      32    4129

  Fragments arranged by size: 

                4129    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2716    2716 
   Size:       5674
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5674
 
PsiI TTA'TAA

Cuts at:   3244    3244 
   Size:       5674
 
PstI C_TGCA'G

Cuts at:    830    2086    2790    3026     830 
   Size:       1256     704     236    3478

  Fragments arranged by size: 

                3478    1256     704     236
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5674
 
PvuII CAG'CTG

Cuts at:   2785    2785 
   Size:       5674
 
SalI G'TCGA_C

Cuts at:   1936    3016    1936 
   Size:       1080    4594
 
SbfI CC_TGCA'GG

Cuts at:   3026    3026 
   Size:       5674
 
ScaI AGT'ACT

Cuts at:   3922    5634    3922 
   Size:       1712    3962
 
SexAI A'CCwGG_T

Cuts at:   2571    2571 
   Size:       5674
 
SfcI C'TryA_G

Cuts at:    826    1045    2082    2650    2786    3022    4157    4838    5029 
   Size:        219    1037     568     136     236    1135     681     191

Cuts at:   5029     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1037     681     568     236     219     191
                 136
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5674
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5674
 
SmaI CCC'GGG

Cuts at:   1872    3001    1872 
   Size:       1129    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2549    3778    4679    4941     820 
   Size:        197     926     606    1229     901     262    1553

  Fragments arranged by size: 

                1553    1229     926     901     606     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3465     493 
   Size:       2972    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5674
 
SphI G_CATG'C

Cuts at:   1132    3032    1132 
   Size:       1900    3774
 
SspI AAT'ATT

Cuts at:      5      52    3598       5 
   Size:         47    3546    2081

  Fragments arranged by size: 

                3546    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5674
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2298    2454    2681    2733    3113     513 
   Size:        552     939     294     156     227      52     380    3074

  Fragments arranged by size: 

                3074     939     552     380     294     227     156      52
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2674    3701    3718    3871    4056    4501     325 
   Size:       1255    1094    1027      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1094    1027     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2553    3920    5632 
   Size:        274      80      33      51    1443     576    1367    1712

Cuts at:   5632      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1367     576     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5634    5666    5634 
   Size:         32    5642
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2720    1316 
   Size:       1404    4270
 
XbaI T'CTAG_A

Cuts at:   2107    3010    2107 
   Size:        903    4771
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2789    2789 
   Size:       5674
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5674
 
XmaI C'CCGG_G

Cuts at:   1870    2999    1870 
   Size:       1129    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3803    3803 
   Size:       5674
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3482     276 
   Size:         53      83     186     936    1948    2468

  Fragments arranged by size: 

                2468    1948     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI     SfiI
     SfoI     SrfI     SwaI
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