(Circular) (Six-base) MAPSORT of: pFN21AE4314.seq Check: 9540 from: 1 to: 5674 pFN21AE4314 5674 bp With 182 enzymes: SgfI * March 28, 2012 14:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2839 2839 Size: 5674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2856 3737 5121 5389 1632 Size: 1224 881 1384 268 1917 Fragments arranged by size: 1917 1384 1224 881 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2161 4573 5550 1203 Size: 958 2412 977 1327 Fragments arranged by size: 2412 1327 977 958 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2366 3417 4989 2366 Size: 1051 1572 3051 Fragments arranged by size: 3051 1572 1051 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2858 2983 3209 3361 5470 1493 Size: 1365 125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1365 226 152 125 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2999 1870 Size: 73 1056 4545 Fragments arranged by size: 4545 1056 73 BamHI G'GATC_C Cuts at: 2663 3004 3371 2663 Size: 341 367 4966 Fragments arranged by size: 4966 367 341 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2995 4450 618 Size: 325 217 627 282 926 1455 1842 Fragments arranged by size: 1842 1455 926 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2550 2993 1391 Size: 1159 443 4072 Fragments arranged by size: 4072 1159 443 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 928 Size: 971 342 4361 Fragments arranged by size: 4361 971 342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 3091 3091 Size: 5674 Bme1580I G_kGCm'C Cuts at: 1790 3735 4891 1790 Size: 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2714 3405 4363 Size: 416 53 83 399 24 1297 691 958 Cuts at: 4363 442 Size: 1753 Fragments arranged by size: 1753 1297 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2543 4334 1239 Size: 1304 1791 2579 Fragments arranged by size: 2579 1791 1304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 3799 4664 4962 2534 Size: 1265 865 298 3246 Fragments arranged by size: 3246 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 1178 Size: 30 165 30 1278 30 4141 Fragments arranged by size: 4141 1278 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2891 1186 Size: 1006 699 3969 Fragments arranged by size: 3969 1006 699 BseYI C'CCAG_C Cuts at: 2758 4877 5373 2758 Size: 2119 496 3059 Fragments arranged by size: 3059 2119 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 2867 1163 Size: 573 406 725 3970 Fragments arranged by size: 3970 725 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2659 2709 3735 3820 4891 2659 Size: 50 1026 85 1071 3442 Fragments arranged by size: 3442 1071 1026 85 50 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2550 2659 2709 2993 3735 3820 4891 Size: 399 760 109 50 284 742 85 1071 Cuts at: 4891 1391 Size: 2174 Fragments arranged by size: 2174 1071 760 742 399 284 109 85 50 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 2839 3029 844 Size: 1329 666 190 3489 Fragments arranged by size: 3489 1329 666 190 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4318 4505 1700 Size: 110 2508 187 2869 Fragments arranged by size: 2869 2508 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 2012 4478 2012 Size: 2466 3208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2431 2663 3004 3371 3756 3773 5225 5311 Size: 459 232 341 367 385 17 1452 86 Cuts at: 5311 5323 5669 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 459 385 367 346 341 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 2681 513 Size: 552 939 450 227 3506 Fragments arranged by size: 3506 939 552 450 227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 2227 3190 3984 4004 2227 Size: 963 794 20 3897 Fragments arranged by size: 3897 963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2481 2808 Size: 54 1425 50 275 18 363 288 327 Cuts at: 2808 3121 4010 5639 8 Size: 313 889 1629 43 Fragments arranged by size: 1629 1425 889 363 327 313 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 3604 1353 Size: 869 1382 3423 Fragments arranged by size: 3423 1382 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 2856 3737 4334 5121 5389 1239 Size: 393 911 313 881 597 787 268 1524 Fragments arranged by size: 1524 911 881 787 597 393 313 268 EcoNI CCTnn'n_nnAGG Cuts at: 2302 2302 Size: 5674 EcoO109I rG'GnC_Cy Cuts at: 2716 2716 Size: 5674 EcoRI G'AATT_C Cuts at: 2858 2983 3361 2858 Size: 125 378 5171 Fragments arranged by size: 5171 378 125 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2681 2713 4315 Size: 32 135 32 609 32 468 32 1602 Cuts at: 4315 4347 4389 4421 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1602 609 468 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 2868 3018 3264 669 Size: 988 281 400 530 150 246 3079 Fragments arranged by size: 3079 988 530 400 281 246 150 HindIII A'AGCT_T Cuts at: 748 2255 748 Size: 1507 4167 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 2324 3273 2324 Size: 949 4725 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2167 2314 2526 4788 4972 1305 Size: 862 147 212 2262 184 2007 Fragments arranged by size: 2262 2007 862 212 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2483 2810 10 Size: 54 1425 343 363 288 327 2874 Fragments arranged by size: 2874 1425 363 343 327 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3632 3991 4150 518 Size: 648 1329 1137 359 159 2042 Fragments arranged by size: 2042 1329 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2785 3090 3767 4915 5160 1606 Size: 1179 305 677 1148 245 2120 Fragments arranged by size: 2120 1179 1148 677 305 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2454 2681 513 Size: 552 939 450 227 3506 Fragments arranged by size: 3506 939 552 450 227 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2165 3032 4577 1132 Size: 97 936 867 1545 2229 Fragments arranged by size: 2229 1545 936 867 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 2161 4573 2161 Size: 2412 3262 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2716 2716 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 2086 2790 3026 830 Size: 1256 704 236 3478 Fragments arranged by size: 3478 1256 704 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2785 2785 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SexAI A'CCwGG_T Cuts at: 2571 2571 Size: 5674 SfcI C'TryA_G Cuts at: 826 1045 2082 2650 2786 3022 4157 4838 5029 Size: 219 1037 568 136 236 1135 681 191 Cuts at: 5029 826 Size: 1471 Fragments arranged by size: 1471 1135 1037 681 568 236 219 191 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2549 3778 4679 4941 820 Size: 197 926 606 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 2298 2454 2681 2733 3113 513 Size: 552 939 294 156 227 52 380 3074 Fragments arranged by size: 3074 939 552 380 294 227 156 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2674 3701 3718 3871 4056 4501 325 Size: 1255 1094 1027 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1094 1027 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2553 3920 5632 Size: 274 80 33 51 1443 576 1367 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1712 1443 1367 576 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 2720 1316 Size: 1404 4270 XbaI T'CTAG_A Cuts at: 2107 3010 2107 Size: 903 4771 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2789 2789 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI