Restriction map for
pFN21AB5920
(Linear) (Six-base) MAPSORT of: KIBB5920.seq Check: 4768 from: 1 to: 1596
KIBB5920.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 349 821 988 1427 1596
Size: 349 472 167 439 169
Fragments arranged by size:
472 439 349 169 167
AccI GT'mk_AC
Cuts at: 0 1352 1596
Size: 1352 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1461 1596
Size: 1461 135
AflIII A'CryG_T
Cuts at: 0 377 845 1596
Size: 377 468 751
Fragments arranged by size:
751 468 377
AhdI GACnn_n'nnGTC
Cuts at: 0 483 1596
Size: 483 1113
AleI CACnn'nnGTG
Cuts at: 0 380 1585 1596
Size: 380 1205 11
Fragments arranged by size:
1205 380 11
AlwNI CAG_nnn'CTG
Cuts at: 0 61 1596
Size: 61 1535
ApaI G_GGCC'C
Cuts at: 0 259 1596
Size: 259 1337
AvaI C'yCGr_G
Cuts at: 0 250 1163 1596
Size: 250 913 433
Fragments arranged by size:
913 433 250
BamHI G'GATC_C
Cuts at: 0 801 1596
Size: 801 795
BanI G'GyrC_C
Cuts at: 0 361 790 809 1596
Size: 361 429 19 787
Fragments arranged by size:
787 429 361 19
BanII G_rGCy'C
Cuts at: 0 221 244 259 405 580 1081 1596
Size: 221 23 15 146 175 501 515
Fragments arranged by size:
515 501 221 175 146 23 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 531 656 1219 1596
Size: 50 481 125 563 377
Fragments arranged by size:
563 481 377 125 50
BbvCI CC'TCA_GC
Cuts at: 0 1228 1596
Size: 1228 368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 795 829 1596
Size: 795 34 767
Fragments arranged by size:
795 767 34
BglI GCCn_nnn'nGGC
Cuts at: 0 767 1596
Size: 767 829
Bme1580I G_kGCm'C
Cuts at: 0 259 793 1596
Size: 259 534 803
Fragments arranged by size:
803 534 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 481 597 1207 1224 1596
Size: 246 235 116 610 17 372
Fragments arranged by size:
610 372 246 235 116 17
Bpu10I CC'TnA_GC
Cuts at: 0 215 1228 1596
Size: 215 1013 368
Fragments arranged by size:
1013 368 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1110 1596
Size: 432 678 486
Fragments arranged by size:
678 486 432
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 615 1596
Size: 415 200 981
Fragments arranged by size:
981 415 200
BsaAI yAC'GTr
Cuts at: 0 378 822 852 1596
Size: 378 444 30 744
Fragments arranged by size:
744 444 378 30
BsaWI w'CCGG_w
Cuts at: 0 39 542 1596
Size: 39 503 1054
Fragments arranged by size:
1054 503 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 181 184 1204 1330 1471 1474 1596
Size: 124 57 3 1020 126 141 3 122
Fragments arranged by size:
1020 141 126 124 122 57 3 3
BseYI C'CCAG_C
Cuts at: 0 54 125 1141 1596
Size: 54 71 1016 455
Fragments arranged by size:
1016 455 71 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 520 1039 1286 1563 1596
Size: 415 105 519 247 277 33
Fragments arranged by size:
519 415 277 247 105 33
BsiHKAI G_wGCw'C
Cuts at: 0 221 405 580 1596
Size: 221 184 175 1016
Fragments arranged by size:
1016 221 184 175
BsmI GAATG_Cn'
Cuts at: 0 678 1596
Size: 678 918
Bsp1286I G_dGCh'C
Cuts at: 0 221 244 259 405 580 793 1081 1596
Size: 221 23 15 146 175 213 288 515
Fragments arranged by size:
515 288 221 213 175 146 23 15
BspEI T'CCGG_A
Cuts at: 0 39 542 1596
Size: 39 503 1054
Fragments arranged by size:
1054 503 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 349 821 988 1427 1520 1596
Size: 329 20 472 167 439 93 76
Fragments arranged by size:
472 439 329 167 93 76 20
BsrBI CCG'CTC
Cuts at: 0 465 1596
Size: 465 1131
BsrDI GCAATG_nn'
Cuts at: 0 1572 1596
Size: 1572 24
BsrFI r'CCGG_y
Cuts at: 0 148 1596
Size: 148 1448
BsrGI T'GTAC_A
Cuts at: 0 317 1025 1596
Size: 317 708 571
Fragments arranged by size:
708 571 317
BssSI C'ACGA_G
Cuts at: 0 481 1254 1522 1596
Size: 481 773 268 74
Fragments arranged by size:
773 481 268 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 361 1596
Size: 361 1235
BstBI TT'CG_AA
Cuts at: 0 924 1596
Size: 924 672
BstYI r'GATC_y
Cuts at: 0 9 692 801 947 1596
Size: 9 683 109 146 649
Fragments arranged by size:
683 649 146 109 9
Bsu36I CC'TnA_GG
Cuts at: 0 107 1596
Size: 107 1489
BtgI C'CryG_G
Cuts at: 0 488 515 1121 1296 1545 1596
Size: 488 27 606 175 249 51
Fragments arranged by size:
606 488 249 175 51 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 302 1596
Size: 302 1294
BtsI GCAGTG_nn'
Cuts at: 0 963 1596
Size: 963 633
EaeI y'GGCC_r
Cuts at: 0 930 1118 1134 1491 1596
Size: 930 188 16 357 105
Fragments arranged by size:
930 357 188 105 16
EarI CTCTTCn'nnn_
Cuts at: 0 1009 1596
Size: 1009 587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 536 988 1596
Size: 536 452 608
Fragments arranged by size:
608 536 452
EcoICRI GAG'CTC
Cuts at: 0 219 403 578 1596
Size: 219 184 175 1018
Fragments arranged by size:
1018 219 184 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 481 597 1207 1224 1461 1596
Size: 246 235 116 610 17 237 135
Fragments arranged by size:
610 246 237 235 135 116 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1189 1596
Size: 1189 407
EcoO109I rG'GnC_Cy
Cuts at: 0 65 122 255 256 332 1596
Size: 65 57 133 1 76 1264
Fragments arranged by size:
1264 133 76 65 57 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 755 787 1596
Size: 469 32 254 32 809
Fragments arranged by size:
809 469 254 32 32
HindIII A'AGCT_T
Cuts at: 0 730 919 1596
Size: 730 189 677
Fragments arranged by size:
730 677 189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1596
Size: 866 730
MscI TGG'CCA
Cuts at: 0 932 1120 1136 1493 1596
Size: 932 188 16 357 103
Fragments arranged by size:
932 357 188 103 16
MslI CAynn'nnrTG
Cuts at: 0 380 1585 1596
Size: 380 1205 11
Fragments arranged by size:
1205 380 11
MspA1I CmG'CkG
Cuts at: 0 617 711 1023 1326 1383 1539 1547 1596
Size: 617 94 312 303 57 156 8 49
Fragments arranged by size:
617 312 303 156 94 57 49 8
NcoI C'CATG_G
Cuts at: 0 1121 1596
Size: 1121 475
PasI CC'CwG_GG
Cuts at: 0 61 1392 1596
Size: 61 1331 204
Fragments arranged by size:
1331 204 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 61 1596
Size: 61 1535
PfoI T'CCnGG_A
Cuts at: 0 1370 1596
Size: 1370 226
PmlI CAC'GTG
Cuts at: 0 378 1596
Size: 378 1218
PpuMI rG'GwC_Cy
Cuts at: 0 65 122 1596
Size: 65 57 1474
Fragments arranged by size:
1474 65 57
PspOMI G'GGCC_C
Cuts at: 0 255 1596
Size: 255 1341
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 553 585 1596
Size: 553 32 1011
Fragments arranged by size:
1011 553 32
PstI C_TGCA'G
Cuts at: 0 226 340 710 818 958 1424 1520 1596
Size: 226 114 370 108 140 466 96 76
Fragments arranged by size:
466 370 226 140 114 108 96 76
PvuII CAG'CTG
Cuts at: 0 617 711 1023 1539 1596
Size: 617 94 312 516 57
Fragments arranged by size:
617 516 312 94 57
SacI G_AGCT'C
Cuts at: 0 221 405 580 1596
Size: 221 184 175 1016
Fragments arranged by size:
1016 221 184 175
SacII CC_GC'GG
Cuts at: 0 1548 1596
Size: 1548 48
SanDI GG'GwC_CC
Cuts at: 0 65 1596
Size: 65 1531
SbfI CC_TGCA'GG
Cuts at: 0 340 1596
Size: 340 1256
SfcI C'TryA_G
Cuts at: 0 222 336 706 814 954 1420 1516 1596
Size: 222 114 370 108 140 466 96 80
Fragments arranged by size:
466 370 222 140 114 108 96 80
SmaI CCC'GGG
Cuts at: 0 1165 1596
Size: 1165 431
SmlI C'TyrA_G
Cuts at: 0 411 1089 1596
Size: 411 678 507
Fragments arranged by size:
678 507 411
StuI AGG'CCT
Cuts at: 0 1201 1596
Size: 1201 395
StyI C'CwwG_G
Cuts at: 0 785 1121 1596
Size: 785 336 475
Fragments arranged by size:
785 475 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1297 1596
Size: 1297 299
TatI w'GTAC_w
Cuts at: 0 317 1025 1472 1596
Size: 317 708 447 124
Fragments arranged by size:
708 447 317 124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 849 1330 1596
Size: 849 481 266
Fragments arranged by size:
849 481 266
XmaI C'CCGG_G
Cuts at: 0 1163 1596
Size: 1163 433
XmnI GAAnn'nnTTC
Cuts at: 0 550 1596
Size: 550 1046
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HindIII MmeI MscI MslI MspA1I
NcoI PasI PflMI PfoI PmlI PpuMI PspOMI PsrI
PstI PvuII SacI SacII SanDI SbfI SfcI SmaI
SmlI StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PshAI PsiI PspXI PvuI
RsrII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI ZraI