(Circular) (Six-base) MAPSORT of: pFN21AB5356.seq Check: 1489 from: 1 to: 6049 pFN21AB5356 6049 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2060 3859 278 Size: 53 83 186 936 524 1799 2468 Fragments arranged by size: 2468 1799 936 524 186 83 53 AccI GT'mk_AC Cuts at: 1937 3392 1937 Size: 1455 4594 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4112 5496 5764 1632 Size: 2480 1384 268 1917 Fragments arranged by size: 2480 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 4948 5925 1203 Size: 3745 977 1327 Fragments arranged by size: 3745 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 3792 5364 3792 Size: 1572 4477 ApaI G_GGCC'C Cuts at: 2117 2675 2117 Size: 558 5491 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3358 3584 3736 5845 1493 Size: 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2210 3095 3374 1870 Size: 73 267 885 279 4545 Fragments arranged by size: 4545 885 279 267 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2774 2942 3370 4825 618 Size: 325 217 627 987 168 428 1455 1842 Fragments arranged by size: 1842 1455 987 627 428 325 217 168 BanII G_rGCy'C Cuts at: 1391 2081 2117 2675 2865 3276 3368 1391 Size: 690 36 558 190 411 92 4072 Fragments arranged by size: 4072 690 558 411 190 92 36 BbeI G_GCGC'C Cuts at: 2778 2778 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 2985 928 Size: 971 621 465 3992 Fragments arranged by size: 3992 971 621 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4240 4274 1903 Size: 34 50 34 2219 34 3678 Fragments arranged by size: 3678 2219 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 2268 2439 2520 2706 1548 Size: 720 171 81 186 4891 Fragments arranged by size: 4891 720 186 171 81 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2103 4660 136 Size: 107 122 71 631 605 431 2557 1525 Fragments arranged by size: 2557 1525 631 605 431 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 2117 2675 2947 4110 5266 1790 Size: 327 558 272 1163 1156 2573 Fragments arranged by size: 2573 1163 1156 558 327 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2134 3780 4738 Size: 416 53 83 399 24 717 1646 958 Cuts at: 4738 442 Size: 1753 Fragments arranged by size: 1753 1646 958 717 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2933 3080 4709 1239 Size: 1010 684 147 1629 2579 Fragments arranged by size: 2579 1629 1010 684 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4174 5039 5337 2564 Size: 1610 865 298 3276 Fragments arranged by size: 3276 1610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2775 3856 4238 Size: 53 83 186 936 524 718 1081 382 Cuts at: 4238 4873 275 Size: 635 1451 Fragments arranged by size: 1451 1081 936 718 635 524 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5824 Fragments arranged by size: 5824 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2189 2399 2923 3068 3083 1186 Size: 991 12 210 524 145 15 4152 Fragments arranged by size: 4152 991 524 210 145 15 12 BseYI C'CCAG_C Cuts at: 5252 5748 5252 Size: 496 5553 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5476 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 2219 3311 3499 4260 5288 Size: 275 186 72 146 1092 188 761 1028 Cuts at: 5288 1540 Size: 2301 Fragments arranged by size: 2301 1092 1028 761 275 188 186 146 72 BsiHKAI G_wGCw'C Cuts at: 2481 2580 4110 4195 5266 2481 Size: 99 1530 85 1071 3264 Fragments arranged by size: 3264 1530 1071 99 85 BsmI GAATG_Cn' Cuts at: 2835 3560 3653 2835 Size: 725 93 5231 Fragments arranged by size: 5231 725 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2117 2481 2580 2675 2865 2947 Size: 399 291 36 364 99 95 190 82 Cuts at: 2947 3276 3368 4110 4195 5266 1391 Size: 329 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 364 329 291 190 99 95 92 85 82 36 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 2436 2703 3938 5668 2436 Size: 267 1235 1730 2817 Fragments arranged by size: 2817 1730 1235 267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3404 844 Size: 2560 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2053 3936 1394 Size: 429 230 1883 3507 Fragments arranged by size: 3507 1883 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 4544 4718 65 Size: 1183 3296 174 1396 Fragments arranged by size: 3296 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 2795 4109 5121 2795 Size: 1314 1012 3723 Fragments arranged by size: 3723 1314 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2655 794 Size: 1861 4188 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstXI CCAn_nnnn'nTGG Cuts at: 2470 2470 Size: 6049 BstYI r'GATC_y Cuts at: 1972 3379 3746 4131 4148 5600 5686 5698 6044 Size: 1407 367 385 17 1452 86 12 346 Cuts at: 6044 1972 Size: 1977 Fragments arranged by size: 1977 1452 1407 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2509 2598 2895 3141 3291 513 Size: 552 1444 89 297 246 150 3271 Fragments arranged by size: 3271 1444 552 297 246 150 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 2649 3565 4359 4379 2649 Size: 916 794 20 4319 Fragments arranged by size: 4319 916 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2295 2295 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2192 5056 809 Size: 507 876 2864 1802 Fragments arranged by size: 2864 1802 876 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2216 2595 Size: 54 1425 50 275 18 240 146 379 Cuts at: 2595 3183 3308 3496 4385 6014 8 Size: 588 125 188 889 1629 43 Fragments arranged by size: 1629 1425 889 588 379 275 240 188 146 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 2216 3308 3496 1537 Size: 275 258 146 1092 188 4090 Fragments arranged by size: 4090 1092 275 258 188 146 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2555 3060 3979 1353 Size: 876 326 505 919 3423 Fragments arranged by size: 3423 919 876 505 326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2933 3080 4112 4709 5496 5764 Size: 393 617 684 147 1032 597 787 268 Cuts at: 5764 1239 Size: 1524 Fragments arranged by size: 1524 1032 787 684 617 597 393 268 147 EcoO109I rG'GnC_Cy Cuts at: 2113 2179 2671 2672 2819 2113 Size: 66 492 1 147 5343 Fragments arranged by size: 5343 492 147 66 1 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2407 2778 3242 5196 1678 Size: 729 371 464 1954 2531 Fragments arranged by size: 2531 1954 729 464 371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2346 2375 2378 Size: 32 135 32 470 32 272 29 3 Cuts at: 2378 2407 2918 2950 4690 4722 4764 4796 1373 Size: 29 511 32 1740 32 42 32 2626 Fragments arranged by size: 2626 1740 511 470 272 135 42 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3393 3639 669 Size: 988 281 1455 246 3079 Fragments arranged by size: 3079 1455 988 281 246 HindIII A'AGCT_T Cuts at: 748 3086 748 Size: 2338 3711 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KasI G'GCGC_C Cuts at: 2774 2774 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5163 5347 1305 Size: 3858 184 2007 Fragments arranged by size: 3858 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2597 3185 10 Size: 54 1425 343 765 588 2874 Fragments arranged by size: 2874 1425 765 588 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2303 2783 4007 4366 4525 518 Size: 648 849 288 480 1224 359 159 2042 Fragments arranged by size: 2042 1224 849 648 480 359 288 159 MspA1I CmG'CkG Cuts at: 1606 2493 3150 3465 4142 5290 5535 1606 Size: 887 657 315 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 657 315 245 NarI GG'CG_CC Cuts at: 2775 2775 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2509 2598 2895 513 Size: 552 1444 89 297 3667 Fragments arranged by size: 3667 1444 552 297 89 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 2216 3496 2216 Size: 1280 4769 NspI r_CATG'y Cuts at: 1132 1229 2267 3407 4952 1132 Size: 97 1038 1140 1545 2229 Fragments arranged by size: 2229 1545 1140 1038 97 PasI CC'CwG_GG Cuts at: 1348 3047 1348 Size: 1699 4350 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2123 2901 1108 Size: 1015 778 4256 Fragments arranged by size: 4256 1015 778 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2179 Size: 6049 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspOMI G'GGCC_C Cuts at: 2113 2671 2113 Size: 558 5491 PstI C_TGCA'G Cuts at: 830 2688 3401 830 Size: 1858 713 3478 Fragments arranged by size: 3478 1858 713 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SapI GCTCTTCn'nnn_ Cuts at: 2229 2229 Size: 6049 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 3300 3300 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 2197 2684 3112 3397 4532 5213 5404 Size: 219 1152 487 428 285 1135 681 191 Cuts at: 5404 826 Size: 1471 Fragments arranged by size: 1471 1152 1135 681 487 428 285 219 191 SfoI GGC'GCC Cuts at: 2776 2776 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 2212 3376 1872 Size: 340 1164 4545 Fragments arranged by size: 4545 1164 340 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 4153 5054 5316 820 Size: 197 926 636 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 636 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 2226 2693 1517 Size: 709 467 4873 Fragments arranged by size: 4873 709 467 StyI C'CwwG_G Cuts at: 513 1065 2509 2566 2598 2895 3488 513 Size: 552 1444 57 32 297 593 3074 Fragments arranged by size: 3074 1444 593 552 297 57 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4076 4093 4246 4431 4876 325 Size: 1255 2496 17 153 185 445 1498 Fragments arranged by size: 2496 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 6009 6041 2027 Size: 32 3950 32 2035 Fragments arranged by size: 3950 2035 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 3298 2470 Size: 828 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 2210 3374 1870 Size: 340 1164 4545 Fragments arranged by size: 4545 1164 340 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2058 3857 276 Size: 53 83 186 936 524 1799 2468 Fragments arranged by size: 2468 1799 936 524 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI