################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssHII BssSI Bst1107I BstZ17I CelII ClaI CpoI Eam1105I Eco47III EcoNI EcoRV FauI FbaI FseI FspI HgaI HpaI MluI MroI NciI NdeI NheI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI SspBI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AcsI 660 GAATTC [GA]^AATT[CT] AcyI 139 GGCGCC G[GA]^CG[CT]C AflIII 283 ACATGT A^C[GA][CT]GT ApoI 660 GAATTC [GA]^AATT[CT] AspHI 1183 GTGCTC G[AT]GC[AT]^C AvaI 155 CTCGAG C^[CT]CG[GA]G BbeI 139 GGCGCC GGCGC^C BbsI 1083 GAAGAC GAAGAC(2/6) BglI 37 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1083 GAAGAC GAAGAC(2/6) BsaBI 75 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 139 GGCGCC G[GA]^CG[CT]C BsgI 2137 GTGCAG GTGCAG(16/14) BsiEI 35 CGGCCG CG[GA][CT]^CG BsiHKAI 1183 GTGCTC G[AT]GC[AT]^C BsmFI 1486 GTCCC GGGAC(10/14) BsoBI 155 CTCGAG C^[CT]CG[GA]G BspHI 1764 TCATGA T^CATGA BspLU11I 283 ACATGT A^CATGT BsrBI 152 CCGCTC CCGCTC(-3/-3) BsrFI 62 GCCGGC [GA]^CCGG[CT] BstAPI 988 GCAGGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 888 GGTAACC G^GT[ACGT]ACC BstPI 888 GGTAACC G^GT[ACGT]ACC BstXI 425 CCATTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 62 GCCGGC [GA]^CCGG[CT] DraI 484 TTTAAA TTT^AAA DraII 1877 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 936 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1879 GACCCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 35 CGGCCG C^GGCCG EclXI 35 CGGCCG C^GGCCG Eco52I 35 CGGCCG C^GGCCG EcoO109I 1877 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 888 GGTAACC G^GT[ACGT]ACC EcoRI 660 GAATTC G^AATTC EcoT22I 16 ATGCAT ATGCA^T Hin1I 139 GGCGCC G[GA]^CG[CT]C HincII 2004 GTCAAC GT[CT]^[GA]AC HindII 2004 GTCAAC GT[CT]^[GA]AC HindIII 1825 AAGCTT A^AGCTT KasI 139 GGCGCC G^GCGCC KspI 38 CCGCGG CCGC^GG MamI 75 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1856 CAATTG C^AATTG MspA1I 38 CCGCGG C[AC]G^C[GT]G MunI 1856 CAATTG C^AATTG NaeI 62 GCCGGC GCC^GGC NarI 139 GGCGCC GG^CGCC NgoMIV 62 GCCGGC G^CCGGC NotI 34 GCGGCCGC GC^GGCCGC NruI 11 TCGCGA TCG^CGA NsiI 16 ATGCAT ATGCA^T PaeR7I 155 CTCGAG C^TCGAG PpuMI 1877 AGGACCC [GA]G^G[AT]CC[CT] PstI 1490 CTGCAG CTGCA^G RcaI 1764 TCATGA T^CATGA SacII 38 CCGCGG CCGC^GG SbfI 1489 CCTGCAGG CCTGCA^GG SfoI 139 GGCGCC GGC^GCC Sse8387I 1489 CCTGCAGG CCTGCA^GG SspI 463 AATATT AAT^ATT Tsp45I 934 GTCAC ^GT[GC]AC XhoI 155 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 496 GTAGAC GT^[AC][GT]AC 1351 GTAGAC AocI 1657 CCTCAGG CC^T[ACGT]AGG 1726 CCTGAGG Asp700I 1627 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2246 GAAACTGTTC AvaII 112 GGTCC G^G[AT]CC 1878 GGACC BalI 262 TGGCCA TGG^CCA 1505 TGGCCA BglII 405 AGATCT A^GATCT 1227 AGATCT BmyI 872 GTGCCC G[AGT]GC[ACT]^C 1183 GTGCTC BpmI 253 CTCCAG CTGGAG(16/14) 605 CTGGAG Bsp1286I 872 GTGCCC G[AGT]GC[ACT]^C 1183 GTGCTC BspMI 988 GCAGGT ACCTGC(4/8) 1207 GCAGGT BsrDI 1989 CATTGC GCAATG(2/0) 370 GCAATG BstF5I 1662 GGATG GGATG(2/0) 1959 GGATG Bsu36I 1657 CCTCAGG CC^T[ACGT]AGG 1726 CCTGAGG EarI 1672 CTCTTC CTCTTC(1/4) 1933 GAAGAG Eco81I 1657 CCTCAGG CC^T[ACGT]AGG 1726 CCTGAGG FokI 1662 GGATG GGATG(9/13) 1959 GGATG HapII 63 CCGG C^CGG 84 CCGG HpaII 63 CCGG C^CGG 84 CCGG Ksp632I 1672 CTCTTC CTCTTC(1/4) 1933 GAAGAG MaeII 1087 ACGT A^CGT 2061 ACGT MaeIII 889 GTAAC ^GT[ACGT]AC 934 GTCAC MluNI 262 TGGCCA TGG^CCA 1505 TGGCCA MscI 262 TGGCCA TGG^CCA 1505 TGGCCA MspI 63 CCGG C^CGG 84 CCGG NcoI 563 CCATGG C^CATGG 2073 CCATGG NspI 283 ACATGT [GA]CATG^[CT] 524 ACATGC XcmI 458 CCATGAATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1952 CCATGAAGGATGTGG XmnI 1627 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2246 GAAACTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI AlwI Asp718I BanI BbvI BfaI BsaJI BseRI BsiYI BslI BsmAI BsmI BsrI BssKI BstBI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI KpnI MaeI MboI MboII MflI MnlI MseI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV NspV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII