(Circular) (Six-base) MAPSORT of: pFN21AE6720.seq Check: 5957 from: 1 to: 6964 pFN21AE6720 6964 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4774 278 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2502 3357 4307 1937 Size: 565 855 950 4594 Fragments arranged by size: 4594 950 855 565 Acc65I G'GTAC_C Cuts at: 3215 3658 4070 4285 3215 Size: 443 412 215 5894 Fragments arranged by size: 5894 443 412 215 AclI AA'CG_TT Cuts at: 1563 5091 5464 1563 Size: 3528 373 3063 Fragments arranged by size: 3528 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3380 3957 5027 6411 6679 1632 Size: 1748 577 1070 1384 268 1917 Fragments arranged by size: 1917 1748 1384 1070 577 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6767 AflIII A'CryG_T Cuts at: 1203 2288 5863 6840 1203 Size: 1085 3575 977 1327 Fragments arranged by size: 3575 1327 1085 977 AgeI A'CCGG_T Cuts at: 5795 5795 Size: 6964 AhdI GACnn_n'nnGTC Cuts at: 5693 5693 Size: 6964 AleI CACnn'nnGTG Cuts at: 1166 3548 1166 Size: 2382 4582 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2319 4157 1184 Size: 1135 1838 3991 Fragments arranged by size: 3991 1838 1135 AlwNI CAG_nnn'CTG Cuts at: 4707 6279 4707 Size: 1572 5392 ApaLI G'TGCA_C Cuts at: 5021 6177 5021 Size: 1156 5808 ApoI r'AATT_y Cuts at: 1493 2665 4273 4499 4651 6760 1493 Size: 1172 1608 226 152 2109 1697 Fragments arranged by size: 2109 1697 1608 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 5518 160 Size: 872 4486 1606 Fragments arranged by size: 4486 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 AvaI C'yCGr_G Cuts at: 1870 1943 2160 4289 1870 Size: 73 217 2129 4545 Fragments arranged by size: 4545 2129 217 73 BamHI G'GATC_C Cuts at: 4294 4661 4294 Size: 367 6597 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2876 3215 3658 3953 Size: 325 217 627 357 732 339 443 295 Cuts at: 3953 4070 4285 5740 618 Size: 117 215 1455 1842 Fragments arranged by size: 1842 1455 732 627 443 357 339 325 295 217 215 117 BanII G_rGCy'C Cuts at: 1391 4283 1391 Size: 2892 4072 BbeI G_GCGC'C Cuts at: 2148 2148 Size: 6964 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3095 928 Size: 971 1196 4797 Fragments arranged by size: 4797 1196 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5155 5189 1903 Size: 34 3218 34 3678 Fragments arranged by size: 3678 3218 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4856 6066 4856 Size: 1210 5754 BclI T'GATC_A Cuts at: 1548 1548 Size: 6964 BfrBI ATG'CAT Cuts at: 2023 2023 Size: 6964 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 5575 136 Size: 107 122 71 631 605 376 3527 1525 Fragments arranged by size: 3527 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 2410 3232 6959 2410 Size: 822 3727 2415 Fragments arranged by size: 3727 2415 822 BlpI GC'TnA_GC Cuts at: 4381 4381 Size: 6964 Bme1580I G_kGCm'C Cuts at: 1790 2881 5025 6181 1790 Size: 1091 2144 1156 2573 Fragments arranged by size: 2573 2144 1156 1091 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6964 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2875 3884 4695 Size: 416 53 83 399 24 1458 1009 811 Cuts at: 4695 5653 442 Size: 958 1753 Fragments arranged by size: 1753 1458 1009 958 811 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2333 2333 Size: 6964 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2631 5624 1239 Size: 1004 388 2993 2579 Fragments arranged by size: 2993 2579 1004 388 Bpu10I CC'TnA_GC Cuts at: 2113 2732 2113 Size: 619 6345 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3335 3812 5089 5954 6252 3335 Size: 477 1277 865 298 4047 Fragments arranged by size: 4047 1277 865 477 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5627 882 Size: 617 4128 2219 Fragments arranged by size: 4128 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4755 493 Size: 711 3551 2702 Fragments arranged by size: 3551 2702 711 BsaBI GATnn'nnATC Cuts at: 2084 6958 2084 Size: 4874 2090 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 4771 5153 5788 Size: 53 83 186 936 612 2626 382 635 Cuts at: 5788 275 Size: 1451 Fragments arranged by size: 2626 1451 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5397 5795 6069 6216 6627 5397 Size: 398 274 147 411 5734 Fragments arranged by size: 5734 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3222 3252 1178 Size: 30 165 30 1819 30 4890 Fragments arranged by size: 4890 1819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 3855 4211 1186 Size: 1139 1530 356 3939 Fragments arranged by size: 3939 1530 1139 356 BseYI C'CCAG_C Cuts at: 2034 2437 6167 6663 2034 Size: 403 3730 496 2335 Fragments arranged by size: 3730 2335 496 403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4163 1163 Size: 573 2427 3964 Fragments arranged by size: 3964 2427 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 4414 5175 6203 1540 Size: 275 186 42 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 3192 5025 5110 6181 3192 Size: 1833 85 1071 3975 Fragments arranged by size: 3975 1833 1071 85 BsmI GAATG_Cn' Cuts at: 2023 2924 3792 4475 4568 2023 Size: 901 868 683 93 4419 Fragments arranged by size: 4419 901 868 683 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2881 3192 4283 5025 5110 6181 1391 Size: 399 1091 311 1091 742 85 1071 2174 Fragments arranged by size: 2174 1091 1091 1071 742 399 311 85 BspEI T'CCGG_A Cuts at: 6627 6627 Size: 6964 BspHI T'CATG_A Cuts at: 3769 4853 6583 3769 Size: 1084 1730 4150 Fragments arranged by size: 4150 1730 1084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 3203 4319 844 Size: 2140 219 1116 3489 Fragments arranged by size: 3489 2140 1116 219 BsrBI CCG'CTC Cuts at: 1394 1823 2159 4851 1394 Size: 429 336 2692 3507 Fragments arranged by size: 3507 2692 429 336 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 3993 5459 5633 65 Size: 1183 1134 1611 1466 174 1396 Fragments arranged by size: 1611 1466 1396 1183 1134 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 5608 5795 1700 Size: 110 257 3541 187 2869 Fragments arranged by size: 3541 2869 257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6964 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6964 BssSI C'ACGA_G Cuts at: 5024 6036 5024 Size: 1012 5952 BstAPI GCAn_nnn'nTGC Cuts at: 794 2999 794 Size: 2205 4759 BstBI TT'CG_AA Cuts at: 2058 2635 3784 5768 2058 Size: 577 1149 1984 3254 Fragments arranged by size: 3254 1984 1149 577 BstEII G'GTnAC_C Cuts at: 1184 2893 1184 Size: 1709 5255 BstXI CCAn_nnnn'nTGG Cuts at: 2437 2437 Size: 6964 BstYI r'GATC_y Cuts at: 1972 2410 2716 3232 4294 4661 5046 5063 6515 Size: 438 306 516 1062 367 385 17 1452 Cuts at: 6515 6601 6613 6959 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1062 516 438 385 367 346 306 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3663 3732 3663 Size: 69 6895 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2568 3420 4078 513 Size: 552 939 39 525 852 658 3399 Fragments arranged by size: 3399 939 852 658 552 525 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2033 487 Size: 683 56 807 5418 Fragments arranged by size: 5418 807 683 56 BtsI GCAGTG_nn' Cuts at: 2129 3014 3654 3753 4480 5274 5294 2129 Size: 885 640 99 727 794 20 3799 Fragments arranged by size: 3799 885 794 727 640 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6929 DraI TTT'AAA Cuts at: 2491 4268 2491 Size: 1777 5187 DraIII CAC_nnn'GTG Cuts at: 2946 2946 Size: 6964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3890 5971 809 Size: 507 2574 2081 1802 Fragments arranged by size: 2574 2081 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2267 3510 Size: 54 1425 50 275 18 210 227 1243 Cuts at: 3510 4411 5300 6929 8 Size: 901 889 1629 43 Fragments arranged by size: 1629 1425 1243 901 889 275 227 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 4411 1537 Size: 275 228 2371 4090 Fragments arranged by size: 4090 2371 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 3683 3933 4894 1353 Size: 2330 250 961 3423 Fragments arranged by size: 3423 2330 961 250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5551 5925 6071 387 Size: 1064 138 3962 374 146 1280 Fragments arranged by size: 3962 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2631 3380 3957 5027 5624 6411 Size: 393 611 388 749 577 1070 597 787 Cuts at: 6411 6679 1239 Size: 268 1524 Fragments arranged by size: 1524 1070 787 749 611 597 577 393 388 268 EcoO109I rG'GnC_Cy Cuts at: 3883 3883 Size: 6964 EcoRI G'AATT_C Cuts at: 2665 4273 4651 2665 Size: 1608 378 4978 Fragments arranged by size: 4978 1608 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2396 2861 2396 Size: 465 6499 FspI TGC'GCA Cuts at: 5470 5470 Size: 6964 HaeII r_GCGC'y Cuts at: 1678 2012 2035 2068 2148 6111 1678 Size: 334 23 33 80 3963 2531 Fragments arranged by size: 3963 2531 334 80 33 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 3303 3335 3373 Size: 32 135 32 547 32 1152 32 38 Cuts at: 3373 3405 5605 5637 5679 5711 1373 Size: 32 2200 32 42 32 2626 Fragments arranged by size: 2626 2200 1152 547 135 42 38 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4011 4308 4554 669 Size: 988 281 2073 297 246 3079 Fragments arranged by size: 3079 2073 988 297 281 246 HindIII A'AGCT_T Cuts at: 748 3830 748 Size: 3082 3882 HpaI GTT'AAC Cuts at: 4554 4554 Size: 6964 KasI G'GCGC_C Cuts at: 2144 2144 Size: 6964 KpnI G_GTAC'C Cuts at: 3219 3662 4074 4289 3219 Size: 443 412 215 5894 Fragments arranged by size: 5894 443 412 215 MfeI C'AATT_G Cuts at: 3861 4563 3861 Size: 702 6262 MluI A'CGCG_T Cuts at: 6840 6840 Size: 6964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3208 6078 6262 1305 Size: 1903 2870 184 2007 Fragments arranged by size: 2870 2007 1903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2269 3512 10 Size: 54 1425 343 437 1243 3462 Fragments arranged by size: 3462 1425 1243 437 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 3326 3548 3798 4922 5281 5440 Size: 648 1135 1025 222 250 1124 359 159 Cuts at: 5440 518 Size: 2042 Fragments arranged by size: 2042 1135 1124 1025 648 359 250 222 159 MspA1I CmG'CkG Cuts at: 1606 2045 4380 5057 6205 6450 1606 Size: 439 2335 677 1148 245 2120 Fragments arranged by size: 2335 2120 1148 677 439 245 NaeI GCC'GGC Cuts at: 2069 2069 Size: 6964 NarI GG'CG_CC Cuts at: 2145 2145 Size: 6964 NcoI C'CATG_G Cuts at: 513 1065 2004 2568 4078 513 Size: 552 939 564 1510 3399 Fragments arranged by size: 3399 1510 939 564 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6964 NgoMIV G'CCGG_C Cuts at: 2067 2067 Size: 6964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6964 NotI GC'GGCC_GC Cuts at: 2040 4411 2040 Size: 2371 4593 NruI TCG'CGA Cuts at: 2018 2018 Size: 6964 NsiI A_TGCA'T Cuts at: 2025 2025 Size: 6964 NspI r_CATG'y Cuts at: 1132 1229 2292 2533 4322 5867 1132 Size: 97 1063 241 1789 1545 2229 Fragments arranged by size: 2229 1789 1545 1063 241 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6964 PciI A'CATG_T Cuts at: 2288 5863 2288 Size: 3575 3389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6964 PmeI GTTT'AAAC Cuts at: 4268 4268 Size: 6964 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3810 3842 5033 5065 6546 6578 3810 Size: 32 1191 32 1481 32 4196 Fragments arranged by size: 4196 1481 1191 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3883 3883 Size: 6964 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6964 PsiI TTA'TAA Cuts at: 4534 4534 Size: 6964 PspXI vC'TCGA_Gb Cuts at: 2160 2160 Size: 6964 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2896 2928 2896 Size: 32 6932 PstI C_TGCA'G Cuts at: 830 3499 4316 830 Size: 2669 817 3478 Fragments arranged by size: 3478 2669 817 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6964 SacII CC_GC'GG Cuts at: 2046 2046 Size: 6964 SalI G'TCGA_C Cuts at: 1936 4306 1936 Size: 2370 4594 SbfI CC_TGCA'GG Cuts at: 3499 4316 3499 Size: 817 6147 ScaI AGT'ACT Cuts at: 5212 6924 5212 Size: 1712 5252 SfcI C'TryA_G Cuts at: 826 1045 2499 3006 3495 4312 5447 6128 6319 Size: 219 1454 507 489 817 1135 681 191 Cuts at: 6319 826 Size: 1471 Fragments arranged by size: 1471 1454 1135 817 681 507 489 219 191 SfoI GGC'GCC Cuts at: 2146 2146 Size: 6964 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6964 SmaI CCC'GGG Cuts at: 1872 4291 1872 Size: 2419 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 3350 3827 5068 5969 6231 Size: 197 926 217 1190 477 1241 901 262 Cuts at: 6231 820 Size: 1553 Fragments arranged by size: 1553 1241 1190 926 901 477 262 217 197 SnaBI TAC'GTA Cuts at: 493 4755 493 Size: 4262 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6964 SphI G_CATG'C Cuts at: 1132 4322 1132 Size: 3190 3774 SspI AAT'ATT Cuts at: 5 52 2470 4888 5 Size: 47 2418 2418 2081 Fragments arranged by size: 2418 2418 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6964 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 2568 2899 4078 4403 513 Size: 552 939 144 420 331 1179 325 3074 Fragments arranged by size: 3074 1179 939 552 420 331 325 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 3377 3821 4991 5008 5161 5346 Size: 1255 858 939 444 1170 17 153 185 Cuts at: 5346 5791 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1170 939 858 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5210 6922 96 Size: 274 80 33 51 1443 3233 1712 138 Fragments arranged by size: 3233 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3492 3524 6924 6956 3492 Size: 32 3400 32 3500 Fragments arranged by size: 3500 3400 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6964 XbaI T'CTAG_A Cuts at: 4300 4300 Size: 6964 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 3964 2470 Size: 1494 5470 XhoI C'TCGA_G Cuts at: 1943 2160 1943 Size: 217 6747 XmaI C'CCGG_G Cuts at: 1870 4289 1870 Size: 2419 4545 XmnI GAAnn'nnTTC Cuts at: 3636 4255 5093 3636 Size: 619 838 5507 Fragments arranged by size: 5507 838 619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4772 276 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI PacI PfoI PmlI PspOMI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI