Restriction map for
pFN21AB6580
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII Cfr10I
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 133 GTATAC GT^[AC][GT]AC
AlwI 1156 GATCC GGATC(4/5)
Asp700I 601 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 730 CCTCAGC CCTCAGC(-5/-2)
BbvI 358 GCAGC GCAGC(8/12)
BglII 318 AGATCT A^GATCT
BseRI 1054 GAGGAG GAGGAG(10/8)
BsgI 923 GTGCAG GTGCAG(16/14)
BsmAI 482 GAGAC GTCTC(1/5)
BsmFI 384 GGGAC GGGAC(10/14)
BspDI 898 ATCGAT AT^CGAT
BssKI 841 CCAGG ^CC[ACGT]GG
Bst1107I 133 GTATAC GTA^TAC
BstF5I 54 GGATG GGATG(2/0)
BstNI 841 CCAGG CC^[AT]GG
BstYI 318 AGATCT [GA]^GATC[CT]
BstZ17I 133 GTATAC GTA^TAC
CfoI 516 GCGC GCG^C
ClaI 898 ATCGAT AT^CGAT
DraI 435 TTTAAA TTT^AAA
EcoRII 841 CCAGG ^CC[AT]GG
Fnu4HI 358 GCAGC GC^[ACGT]GC
FokI 54 GGATG GGATG(9/13)
HaeII 515 GGCGCT [GA]GCGC^[CT]
HaeIII 450 GGCC GG^CC
HhaI 516 GCGC GCG^C
HinP1I 516 GCGC G^CGC
ItaI 358 GCAGC GC^[ACGT]GC
MflI 318 AGATCT [GA]^GATC[CT]
MvaI 841 CCAGG CC^[AT]GG
NheI 1075 GCTAGC G^CTAGC
PleI 1150 GACTC GAGTC(4/5)
PpuMI 687 AGGACCC [GA]G^G[AT]CC[CT]
PstI 113 CTGCAG CTGCA^G
ScaI 948 AGTACT AGT^ACT
ScrFI 841 CCAGG CC^[ACGT]GG
SfaNI 789 GATGC GCATC(5/9)
SfcI 113 CTGCAG C^T[GA][CT]AG
SspI 145 AATATT AAT^ATT
TseI 358 GCAGC G^C[AT]GC
Tsp45I 1109 GTGAC ^GT[GC]AC
XhoII 318 AGATCT [GA]^GATC[CT]
XmnI 601 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 233 CCGC CCGC(-3/-1)
623 GCGG
BbsI 271 GAAGAC GAAGAC(2/6)
703 GAAGAC
BclI 888 TGATCA T^GATCA
1122 TGATCA
BpuAI 271 GAAGAC GAAGAC(2/6)
703 GAAGAC
BsaBI 343 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1177 GATGAAGATC
DraII 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
687 AGGACCC
EcoO109I 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
687 AGGACCC
EcoRV 44 GATATC GAT^ATC
754 GATATC
FbaI 888 TGATCA T^GATCA
1122 TGATCA
MamI 343 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1177 GATGAAGATC
MwoI 442 GCACAAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
623 GCGGAACTTGC
NlaIII 352 CATG CATG^
714 CATG
NlaIV 688 GGACCC GG[ACGT]^[ACGT]CC
1056 GGAGCC
TaqI 62 TCGA T^CGA
899 TCGA
TthHB8I 62 TCGA T^CGA
899 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BfaI BsiYI BslI BsrI Cac8I
Cfr13I DdeI DpnII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI NdeII RsaI Sau3AI Sau96I
TfiI Tru9I Tsp509I TspRI