################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 223 GTCTAC GT^[AC][GT]AC AciI 67 CCGC CCGC(-3/-1) AfaI 3 GTAC GT^AC BbvI 327 GCAGC GCAGC(8/12) BcgI 43 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 217 GTGCCC G[AGT]GC[ACT]^C BsaJI 269 CCATGG C^C[ACGT][ACGT]GG BsaWI 260 ACCGGA [AT]^CCGG[AT] BsiYI 312 CCTCTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 312 CCTCTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 289 GGGAC GGGAC(10/14) Bsp1286I 217 GTGCCC G[AGT]GC[ACT]^C CfoI 274 GCGC GCG^C DpnII 306 GATC ^GATC DsaI 269 CCATGG C^C[GA][CT]GG EcoT14I 269 CCATGG C^C[AT][AT]GG FauI 66 CCCGC CCCGC(4/6) Fnu4HI 327 GCAGC GC^[ACGT]GC HaeII 273 GGCGCT [GA]GCGC^[CT] HapII 261 CCGG C^CGG HhaI 274 GCGC GCG^C HinP1I 274 GCGC G^CGC HinfI 142 GATTC G^A[ACGT]TC HpaII 261 CCGG C^CGG ItaI 327 GCAGC GC^[ACGT]GC MboI 306 GATC ^GATC MseI 76 TTAA T^TAA MspI 261 CCGG C^CGG MwoI 49 GCTTCGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 269 CCATGG C^CATGG NdeII 306 GATC ^GATC NlaIV 289 GGGACC GG[ACGT]^[ACGT]CC RsaI 3 GTAC GT^AC Sau3AI 306 GATC ^GATC SfcI 60 CTATAG C^T[GA][CT]AG StyI 269 CCATGG C^C[AT][AT]GG TfiI 142 GATTC G^A[AT]TC Tru9I 76 TTAA T^TAA TseI 327 GCAGC G^C[AT]GC TspRI 149 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 201 CCAGAAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AluI 185 AGCT AG^CT 207 AGCT AvaII 263 GGACC G^G[AT]CC 290 GGACC Cac8I 65 GCCCGC GC[ACGT]^[ACGT]GC 239 GCCTGC Cfr13I 263 GGACC G^G[ACGT]CC 290 GGACC HaeIII 57 GGCC GG^CC 238 GGCC Sau96I 263 GGACC G^G[ACGT]CC 290 GGACC StuI 56 AGGCCT AGG^CCT 237 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI NlaIII