(Circular) (Six-base) MAPSORT of: pFN21AE6120.seq Check: 2578 from: 1 to: 5047 pFN21AE6120 5047 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2857 278 Size: 53 83 186 936 1321 2468 Fragments arranged by size: 2468 1321 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2390 1937 Size: 292 161 4594 Fragments arranged by size: 4594 292 161 Acc65I G'GTAC_C Cuts at: 2368 2368 Size: 5047 AclI AA'CG_TT Cuts at: 1563 3174 3547 1563 Size: 1611 373 3063 Fragments arranged by size: 3063 1611 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3110 4494 4762 1632 Size: 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4850 AflIII A'CryG_T Cuts at: 1203 3946 4923 1203 Size: 2743 977 1327 Fragments arranged by size: 2743 1327 977 AgeI A'CCGG_T Cuts at: 3878 3878 Size: 5047 AhdI GACnn_n'nnGTC Cuts at: 3776 3776 Size: 5047 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5047 AlwNI CAG_nnn'CTG Cuts at: 2790 4362 2790 Size: 1572 3475 ApaLI G'TGCA_C Cuts at: 3104 4260 3104 Size: 1156 3891 ApoI r'AATT_y Cuts at: 1493 2356 2582 2734 4843 1493 Size: 863 226 152 2109 1697 Fragments arranged by size: 2109 1697 863 226 152 AseI AT'TA_AT Cuts at: 160 1032 3601 160 Size: 872 2569 1606 Fragments arranged by size: 2569 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5047 AvaI C'yCGr_G Cuts at: 1870 1943 2372 1870 Size: 73 429 4545 Fragments arranged by size: 4545 429 73 BamHI G'GATC_C Cuts at: 2377 2744 2377 Size: 367 4680 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 3823 618 Size: 325 217 627 581 1455 1842 Fragments arranged by size: 1842 1455 627 581 325 217 BanII G_rGCy'C Cuts at: 1391 2366 1391 Size: 975 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4076 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2037 2071 3238 3272 1903 Size: 34 100 34 1167 34 3678 Fragments arranged by size: 3678 1167 100 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2939 4149 2939 Size: 1210 3837 BclI T'GATC_A Cuts at: 1548 1548 Size: 5047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3658 136 Size: 107 122 71 631 605 1986 1525 Fragments arranged by size: 1986 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5042 5042 Size: 5047 BlpI GC'TnA_GC Cuts at: 2464 2464 Size: 5047 Bme1580I G_kGCm'C Cuts at: 1790 2226 3108 4264 1790 Size: 436 882 1156 2573 Fragments arranged by size: 2573 1156 882 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2778 3736 442 Size: 416 53 83 399 24 1361 958 1753 Fragments arranged by size: 1753 1361 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5047 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3707 1239 Size: 2468 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3172 4037 4335 3172 Size: 865 298 3884 Fragments arranged by size: 3884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3710 882 Size: 617 2211 2219 Fragments arranged by size: 2219 2211 617 BsaAI yAC'GTr Cuts at: 493 1204 2838 493 Size: 711 1634 2702 Fragments arranged by size: 2702 1634 711 BsaBI GATnn'nnATC Cuts at: 5041 5041 Size: 5047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2854 3236 3871 275 Size: 53 83 186 936 1321 382 635 1451 Fragments arranged by size: 1451 1321 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 3480 3878 4152 4299 4710 2265 Size: 1215 398 274 147 411 2602 Fragments arranged by size: 2602 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4822 Fragments arranged by size: 4822 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5047 BseYI C'CCAG_C Cuts at: 2168 4250 4746 2168 Size: 2082 496 2469 Fragments arranged by size: 2469 2082 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2497 3258 4286 1540 Size: 275 186 496 761 1028 2301 Fragments arranged by size: 2301 1028 761 496 275 186 BsiHKAI G_wGCw'C Cuts at: 3108 3193 4264 3108 Size: 85 1071 3891 Fragments arranged by size: 3891 1071 85 BsmI GAATG_Cn' Cuts at: 2558 2651 2558 Size: 93 4954 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2366 3108 3193 4264 1391 Size: 399 436 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 140 85 BspEI T'CCGG_A Cuts at: 4710 4710 Size: 5047 BspHI T'CATG_A Cuts at: 2936 4666 2936 Size: 1730 3317 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2402 844 Size: 1558 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2934 1394 Size: 429 1111 3507 Fragments arranged by size: 3507 1111 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3542 3716 65 Size: 1183 2294 174 1396 Fragments arranged by size: 2294 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3691 3878 1700 Size: 110 1881 187 2869 Fragments arranged by size: 2869 1881 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5047 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5047 BssSI C'ACGA_G Cuts at: 3107 4119 3107 Size: 1012 4035 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5047 BstBI TT'CG_AA Cuts at: 3851 3851 Size: 5047 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5047 BstYI r'GATC_y Cuts at: 1972 2377 2744 3129 3146 4598 4684 4696 5042 Size: 405 367 385 17 1452 86 12 346 Cuts at: 5042 1972 Size: 1977 Fragments arranged by size: 1977 1452 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2274 513 Size: 552 1209 3286 Fragments arranged by size: 3286 1209 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4308 Fragments arranged by size: 4308 683 56 BtsI GCAGTG_nn' Cuts at: 2563 3357 3377 2563 Size: 794 20 4233 Fragments arranged by size: 4233 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5047 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2002 2037 529 Size: 35 1438 35 3539 Fragments arranged by size: 3539 1438 35 35 DraI TTT'AAA Cuts at: 2351 2351 Size: 5047 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4054 809 Size: 507 2738 1802 Fragments arranged by size: 2738 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 3383 5012 Size: 54 1425 50 275 18 664 889 1629 Cuts at: 5012 8 Size: 43 Fragments arranged by size: 1629 1425 889 664 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2494 1537 Size: 275 682 4090 Fragments arranged by size: 4090 682 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2977 1353 Size: 1624 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3634 4008 4154 387 Size: 1064 138 2045 374 146 1280 Fragments arranged by size: 2045 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3110 3707 4494 4762 1239 Size: 393 1478 597 787 268 1524 Fragments arranged by size: 1524 1478 787 597 393 268 EcoRI G'AATT_C Cuts at: 2356 2734 2356 Size: 378 4669 FspI TGC'GCA Cuts at: 3553 3553 Size: 5047 HaeII r_GCGC'y Cuts at: 1678 2282 4194 1678 Size: 604 1912 2531 Fragments arranged by size: 2531 1912 604 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 3688 3720 3762 Size: 32 135 32 711 32 1373 32 42 Cuts at: 3762 3794 1373 Size: 32 2626 Fragments arranged by size: 2626 1373 711 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2391 2637 669 Size: 988 281 453 246 3079 Fragments arranged by size: 3079 988 453 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5047 HpaI GTT'AAC Cuts at: 2637 2637 Size: 5047 KpnI G_GTAC'C Cuts at: 2372 2372 Size: 5047 MfeI C'AATT_G Cuts at: 2646 2646 Size: 5047 MluI A'CGCG_T Cuts at: 4923 4923 Size: 5047 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4161 4345 1305 Size: 2856 184 2007 Fragments arranged by size: 2856 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3225 Fragments arranged by size: 3225 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3005 3364 3523 518 Size: 648 1839 359 159 2042 Fragments arranged by size: 2042 1839 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2463 3140 4288 4533 1606 Size: 857 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 677 245 NcoI C'CATG_G Cuts at: 513 1065 2274 513 Size: 552 1209 3286 Fragments arranged by size: 3286 1209 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5047 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5047 NotI GC'GGCC_GC Cuts at: 2494 2494 Size: 5047 NspI r_CATG'y Cuts at: 1132 1229 2405 3950 1132 Size: 97 1176 1545 2229 Fragments arranged by size: 2229 1545 1176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5047 PciI A'CATG_T Cuts at: 3946 3946 Size: 5047 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5047 PmeI GTTT'AAAC Cuts at: 2351 2351 Size: 5047 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3116 3148 4629 4661 3116 Size: 32 1481 32 3502 Fragments arranged by size: 3502 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5047 PsiI TTA'TAA Cuts at: 2617 2617 Size: 5047 PstI C_TGCA'G Cuts at: 830 2399 830 Size: 1569 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5047 SalI G'TCGA_C Cuts at: 1936 2389 1936 Size: 453 4594 SbfI CC_TGCA'GG Cuts at: 2399 2399 Size: 5047 ScaI AGT'ACT Cuts at: 3295 5007 3295 Size: 1712 3335 SfcI C'TryA_G Cuts at: 826 1045 2065 2395 3530 4211 4402 826 Size: 219 1020 330 1135 681 191 1471 Fragments arranged by size: 1471 1135 1020 681 330 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5047 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5047 SmaI CCC'GGG Cuts at: 1872 2374 1872 Size: 502 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3151 4052 4314 820 Size: 197 926 1208 901 262 1553 Fragments arranged by size: 1553 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2838 493 Size: 2345 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5047 SphI G_CATG'C Cuts at: 1132 2405 1132 Size: 1273 3774 SspI AAT'ATT Cuts at: 5 52 2971 5 Size: 47 2919 2081 Fragments arranged by size: 2919 2081 47 StuI AGG'CCT Cuts at: 1517 2063 2244 1517 Size: 546 181 4320 Fragments arranged by size: 4320 546 181 StyI C'CwwG_G Cuts at: 513 1065 2274 2486 513 Size: 552 1209 212 3074 Fragments arranged by size: 3074 1209 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 3091 3244 3429 3874 325 Size: 1255 1494 17 153 185 445 1498 Fragments arranged by size: 1498 1494 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3293 5005 Size: 274 80 33 51 1443 30 1286 1712 Cuts at: 5005 96 Size: 138 Fragments arranged by size: 1712 1443 1286 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5007 5039 5007 Size: 32 5015 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5047 XbaI T'CTAG_A Cuts at: 2383 2383 Size: 5047 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2213 2213 Size: 5047 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5047 XmaI C'CCGG_G Cuts at: 1870 2372 1870 Size: 502 4545 XmnI GAAnn'nnTTC Cuts at: 3176 3176 Size: 5047 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2855 276 Size: 53 83 186 936 1321 2468 Fragments arranged by size: 2468 1321 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI