################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 295 CCGC CCGC(-3/-1) AflIII 545 ACATGT A^C[GA][CT]GT AlwI 755 GGATC GGATC(4/5) AlwNI 380 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 461 CCTCAGG CC^T[ACGT]AGG ApaI 687 GGGCCC GGGCC^C BanI 46 GGTGCC G^G[CT][GA]CC BfaI 554 CTAG C^TAG BpmI 348 CTCCAG CTGGAG(16/14) BsaWI 746 ACCGGA [AT]^CCGG[AT] BseRI 487 GAGGAG GAGGAG(10/8) BsmAI 66 GTCTC GTCTC(1/5) BsmI 99 GCATTC GAATGC(1/-1) BspLU11I 545 ACATGT A^CATGT BsrBI 295 CCGCTC CCGCTC(-3/-3) BsrDI 193 CATTGC GCAATG(2/0) Bsu36I 461 CCTCAGG CC^T[ACGT]AGG CfoI 498 GCGC GCG^C DraIII 143 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 461 CCTCAGG CC^T[ACGT]AGG EcoT14I 731 CCAAGG C^C[AT][AT]GG FauI 294 CCCGC CCCGC(4/6) HapII 747 CCGG C^CGG HhaI 498 GCGC GCG^C HinP1I 498 GCGC G^CGC HpaII 747 CCGG C^CGG MaeI 554 CTAG C^TAG MaeIII 574 GTGAC ^GT[ACGT]AC MslI 729 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 185 CAGCTG C[AC]G^C[GT]G MspI 747 CCGG C^CGG NspI 545 ACATGT [GA]CATG^[CT] PvuII 185 CAGCTG CAG^CTG SexAI 8 ACCTGGT A^CC[AT]GGT StyI 731 CCAAGG C^C[AT][AT]GG TaqI 479 TCGA T^CGA TfiI 634 GATTC G^A[AT]TC Tsp45I 574 GTGAC ^GT[GC]AC TthHB8I 479 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 126 GTAC GT^AC 726 GTAC Alw44I 39 GTGCAC G^TGCAC 163 GTGCAC ApaLI 39 GTGCAC G^TGCAC 163 GTGCAC BanII 598 GGGCTC G[GA]GC[CT]^C 687 GGGCCC Bsp1407I 125 TGTACA T^GTACA 725 TGTACA BspMI 451 ACCTGC ACCTGC(4/8) 114 GCAGGT BsrGI 125 TGTACA T^GTACA 725 TGTACA BstF5I 60 CATCC GGATG(2/0) 136 GGATG DpnII 393 GATC ^GATC 756 GATC FokI 60 CATCC GGATG(9/13) 136 GGATG HphI 573 GGTGA GGTGA(8/7) 631 GGTGA MboI 393 GATC ^GATC 756 GATC NdeII 393 GATC ^GATC 756 GATC PleI 346 GACTC GAGTC(4/5) 507 GAGTC RsaI 126 GTAC GT^AC 726 GTAC Sau3AI 393 GATC ^GATC 756 GATC SfaNI 321 GCATC GCATC(5/9) 476 GCATC SspBI 125 TGTACA T^GTACA 725 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI AspHI AvaII BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HinfI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV PpuMI Sau96I ScrFI TseI TspRI