Restriction map for
pFN21AE3635
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI DsaI Eam1105I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1348 GACGTC GACGT^C
AccIII 278 TCCGGA T^CCGGA
AcyI 1348 GACGTC G[GA]^CG[CT]C
AflIII 118 ACATGT A^C[GA][CT]GT
AocI 1101 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1355 AGCGCT AGC^GCT
Asp700I 228 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 857 TGGCCA TGG^CCA
BanI 107 GGCACC G^G[CT][GA]CC
BbsI 130 GTCTTC GAAGAC(2/6)
BbvCI 534 CCTCAGC CCTCAGC(-5/-2)
BpuAI 130 GTCTTC GAAGAC(2/6)
BsaHI 1348 GACGTC G[GA]^CG[CT]C
BseAI 278 TCCGGA T^CCGGA
BsiEI 1334 CGGCCG CG[GA][CT]^CG
BsmAI 880 GTCTC GTCTC(1/5)
BspEI 278 TCCGGA T^CCGGA
BspHI 551 TCATGA T^CATGA
BspLU11I 118 ACATGT A^CATGT
BspMI 1032 GCAGGT ACCTGC(4/8)
BsrBI 270 GAGCGG CCGCTC(-3/-3)
BssSI 1291 CTCGTG CACGAG(-5/-1)
Bsu36I 1101 CCTAAGG CC^T[ACGT]AGG
DraIII 1117 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1334 CGGCCG C^GGCCG
EclXI 1334 CGGCCG C^GGCCG
Eco47III 1355 AGCGCT AGC^GCT
Eco52I 1334 CGGCCG C^GGCCG
Eco81I 1101 CCTAAGG CC^T[ACGT]AGG
EcoNI 534 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 149 CCTTGG C^C[AT][AT]GG
HaeII 1355 AGCGCT [GA]GCGC^[CT]
Hin1I 1348 GACGTC G[GA]^CG[CT]C
MluNI 857 TGGCCA TGG^CCA
MroI 278 TCCGGA T^CCGGA
MscI 857 TGGCCA TGG^CCA
PflMI 516 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 394 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 551 TCATGA T^CATGA
SacI 139 GAGCTC GAGCT^C
ScaI 559 AGTACT AGT^ACT
SexAI 686 ACCTGGT A^CC[AT]GGT
SphI 420 GCATGC GCATG^C
StuI 380 AGGCCT AGG^CCT
StyI 149 CCTTGG C^C[AT][AT]GG
TaqI 1298 TCGA T^CGA
TthHB8I 1298 TCGA T^CGA
Van91I 516 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 228 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 1272 CGCG CG^CG
1274 CGCG
AcsI 61 GAATTC [GA]^AATT[CT]
232 GAATTC
AlwI 1170 GATCC GGATC(4/5)
281 GGATC
AlwNI 604 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1231 CAGTTCCTG
ApoI 61 GAATTC [GA]^AATT[CT]
232 GAATTC
AvaI 425 CCCGGG C^[CT]CG[GA]G
1040 CCCGGG
BsaWI 278 TCCGGA [AT]^CCGG[AT]
1202 TCCGGT
BseRI 532 CTCCTC GAGGAG(10/8)
457 GAGGAG
BsgI 503 GTGCAG GTGCAG(16/14)
1051 GTGCAG
BsoBI 425 CCCGGG C^[CT]CG[GA]G
1040 CCCGGG
BsrDI 357 CATTGC GCAATG(2/0)
981 CATTGC
BstEII 1035 GGTGACC G^GT[ACGT]ACC
1326 GGTGACC
BstPI 1035 GGTGACC G^GT[ACGT]ACC
1326 GGTGACC
BstUI 1272 CGCG CG^CG
1274 CGCG
BstYI 281 GGATCT [GA]^GATC[CT]
1169 AGATCC
DpnII 282 GATC ^GATC
1170 GATC
DraII 71 GGGGCCT [GA]G^G[ACGT]CC[CT]
317 AGGGCCT
EcoO109I 71 GGGGCCT [GA]G^G[ACGT]CC[CT]
317 AGGGCCT
EcoO65I 1035 GGTGACC G^GT[ACGT]ACC
1326 GGTGACC
EcoRI 61 GAATTC G^AATTC
232 GAATTC
FauI 308 GCGGG CCCGC(4/6)
1275 GCGGG
MaeII 431 ACGT A^CGT
1349 ACGT
MboI 282 GATC ^GATC
1170 GATC
MflI 281 GGATCT [GA]^GATC[CT]
1169 AGATCC
MvnI 1272 CGCG CG^CG
1274 CGCG
NdeII 282 GATC ^GATC
1170 GATC
NspI 118 ACATGT [GA]CATG^[CT]
420 GCATGC
PleI 1199 GACTC GAGTC(4/5)
958 GAGTC
PstI 55 CTGCAG CTGCA^G
967 CTGCAG
Sau3AI 282 GATC ^GATC
1170 GATC
SmaI 425 CCCGGG CCC^GGG
1040 CCCGGG
Tsp45I 1036 GTGAC ^GT[GC]AC
1327 GTGAC
XcmI 1016 CCAACATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1310 CCAAGCGGCTCATGG
XhoII 281 GGATCT [GA]^GATC[CT]
1169 AGATCC
XmaCI 425 CCCGGG C^CCGGG
1040 CCCGGG
XmaI 425 CCCGGG C^CCGGG
1040 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CelII CfoI Cfr13I DdeI EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PvuII
RsaI Sau96I ScrFI SfaNI SfcI
TfiI TseI Tsp509I TspRI