################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 675 GACGCC G[GA]^CG[CT]C AflII 961 CTTAAG C^TTAAG AflIII 732 ACGTGT A^C[GA][CT]GT AlwI 957 GGATC GGATC(4/5) AlwNI 153 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 258 CCTCAGG CC^T[ACGT]AGG Asp700I 310 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BclI 372 TGATCA T^GATCA BfaI 727 CTAG C^TAG BfrI 961 CTTAAG C^TTAAG BsaAI 972 TACGTG [CT]AC^GT[GA] BsaHI 675 GACGCC G[GA]^CG[CT]C BsaWI 436 TCCGGT [AT]^CCGG[AT] BseRI 256 CTCCTC GAGGAG(10/8) BsmI 214 GAATGC GAATGC(1/-1) BspMI 797 GCAGGT ACCTGC(4/8) BsrFI 67 ACCGGC [GA]^CCGG[CT] BstYI 957 GGATCT [GA]^GATC[CT] Bsu36I 258 CCTCAGG CC^T[ACGT]AGG Cfr10I 67 ACCGGC [GA]^CCGG[CT] DrdI 514 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 258 CCTCAGG CC^T[ACGT]AGG EcoNI 391 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 586 CCTTGG C^C[AT][AT]GG FbaI 372 TGATCA T^GATCA HgaI 675 GACGC GACGC(5/10) Hin1I 675 GACGCC G[GA]^CG[CT]C HincII 124 GTTAAC GT[CT]^[GA]AC HindII 124 GTTAAC GT[CT]^[GA]AC HindIII 949 AAGCTT A^AGCTT HpaI 124 GTTAAC GTT^AAC HphI 944 TCACC GGTGA(8/7) MaeI 727 CTAG C^TAG MflI 957 GGATCT [GA]^GATC[CT] MslI 714 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 1223 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 843 GAGTC GAGTC(4/5) PpuMI 799 AGGTCCC [GA]G^G[AT]CC[CT] SacI 828 GAGCTC GAGCT^C StyI 586 CCTTGG C^C[AT][AT]GG TaqI 1144 TCGA T^CGA TthHB8I 1144 TCGA T^CGA Van91I 1223 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 957 GGATCT [GA]^GATC[CT] XmnI 310 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 550 GCGG CCGC(-3/-1) 1329 GCGG AcsI 91 GAATTT [GA]^AATT[CT] 664 GAATTT AfaI 858 GTAC GT^AC 1281 GTAC ApaI 582 GGGCCC GGGCC^C 1274 GGGCCC ApoI 91 GAATTT [GA]^AATT[CT] 664 GAATTT AspHI 442 GTGCTC G[AT]GC[AT]^C 828 GAGCTC AspI 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 937 GACATCGTC BalI 536 TGGCCA TGG^CCA 1220 TGGCCA BsiHKAI 442 GTGCTC G[AT]GC[AT]^C 828 GAGCTC BsmFI 1046 GGGAC GGGAC(10/14) 801 GTCCC BstXI 713 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 947 CCAAGCTTCTGG EaeI 536 TGGCCA [CT]^GGCC[GA] 1220 TGGCCA MaeII 732 ACGT A^CGT 973 ACGT MluNI 536 TGGCCA TGG^CCA 1220 TGGCCA MscI 536 TGGCCA TGG^CCA 1220 TGGCCA MspA1I 577 CAGCTG C[AC]G^C[GT]G 1024 CAGCTG NlaIII 720 CATG CATG^ 1224 CATG PflFI 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 937 GACATCGTC PvuII 577 CAGCTG CAG^CTG 1024 CAGCTG RsaI 858 GTAC GT^AC 1281 GTAC Tsp45I 164 GTCAC ^GT[GC]AC 943 GTCAC Tth111I 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 937 GACATCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AluI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp509I TspRI