(Linear) (Six-base) MAPSORT of: KIEE1544.seq Check: 2279 from: 1 to: 1350 KIEE1544.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1177 1350 Size: 1177 173 AflII C'TTAA_G Cuts at: 0 961 1350 Size: 961 389 AflIII A'CryG_T Cuts at: 0 732 1350 Size: 732 618 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 500 532 1350 Size: 500 32 818 Fragments arranged by size: 818 500 32 AlwNI CAG_nnn'CTG Cuts at: 0 158 1350 Size: 158 1192 ApaI G_GGCC'C Cuts at: 0 586 1278 1350 Size: 586 692 72 Fragments arranged by size: 692 586 72 ApoI r'AATT_y Cuts at: 0 91 664 1350 Size: 91 573 686 Fragments arranged by size: 686 573 91 BanII G_rGCy'C Cuts at: 0 586 832 1278 1350 Size: 586 246 446 72 Fragments arranged by size: 586 446 246 72 BbsI GAAGACnn'nnnn_ Cuts at: 0 317 926 1165 1350 Size: 317 609 239 185 Fragments arranged by size: 609 317 239 185 BclI T'GATC_A Cuts at: 0 372 1350 Size: 372 978 Bme1580I G_kGCm'C Cuts at: 0 152 586 1278 1350 Size: 152 434 692 72 Fragments arranged by size: 692 434 152 72 BmrI ACTGGGnnnn_n' Cuts at: 0 1281 1350 Size: 1281 69 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 733 1350 Size: 733 617 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 510 592 1350 Size: 479 31 82 758 Fragments arranged by size: 758 479 82 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 808 1350 Size: 61 747 542 Fragments arranged by size: 747 542 61 BsaAI yAC'GTr Cuts at: 0 974 1350 Size: 974 376 BsaHI Gr'CG_yC Cuts at: 0 676 1350 Size: 676 674 BsaWI w'CCGG_w Cuts at: 0 436 1350 Size: 436 914 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 452 482 500 530 958 988 1350 Size: 452 30 18 30 428 30 362 Fragments arranged by size: 452 428 362 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 247 1350 Size: 247 1103 BseYI C'CCAG_C Cuts at: 0 579 1350 Size: 579 771 BsiHKAI G_wGCw'C Cuts at: 0 446 832 1350 Size: 446 386 518 Fragments arranged by size: 518 446 386 BsmI GAATG_Cn' Cuts at: 0 220 1350 Size: 220 1130 Bsp1286I G_dGCh'C Cuts at: 0 152 446 586 832 1278 1350 Size: 152 294 140 246 446 72 Fragments arranged by size: 446 294 246 152 140 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 788 1350 Size: 788 562 BsrFI r'CCGG_y Cuts at: 0 67 1350 Size: 67 1283 BstXI CCAn_nnnn'nTGG Cuts at: 0 720 954 1350 Size: 720 234 396 Fragments arranged by size: 720 396 234 BstYI r'GATC_y Cuts at: 0 957 1350 Size: 957 393 Bsu36I CC'TnA_GG Cuts at: 0 259 1350 Size: 259 1091 BtsI GCAGTG_nn' Cuts at: 0 102 165 473 988 1350 Size: 102 63 308 515 362 Fragments arranged by size: 515 362 308 102 63 DrdI GACnn_nn'nnGTC Cuts at: 0 520 1350 Size: 520 830 EaeI y'GGCC_r Cuts at: 0 536 1220 1350 Size: 536 684 130 Fragments arranged by size: 684 536 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 565 1350 Size: 565 785 EcoICRI GAG'CTC Cuts at: 0 830 1350 Size: 830 520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 510 592 1177 1350 Size: 479 31 82 585 173 Fragments arranged by size: 585 479 173 82 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 395 1350 Size: 395 955 EcoO109I rG'GnC_Cy Cuts at: 0 583 800 1275 1350 Size: 583 217 475 75 Fragments arranged by size: 583 475 217 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1152 1184 1350 Size: 1152 32 166 Fragments arranged by size: 1152 166 32 HincII GTy'rAC Cuts at: 0 126 1350 Size: 126 1224 HindIII A'AGCT_T Cuts at: 0 949 1350 Size: 949 401 HpaI GTT'AAC Cuts at: 0 126 1350 Size: 126 1224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1034 1350 Size: 1034 316 MscI TGG'CCA Cuts at: 0 538 1222 1350 Size: 538 684 128 Fragments arranged by size: 684 538 128 MslI CAynn'nnrTG Cuts at: 0 718 1350 Size: 718 632 MspA1I CmG'CkG Cuts at: 0 579 1026 1350 Size: 579 447 324 Fragments arranged by size: 579 447 324 PflMI CCAn_nnn'nTGG Cuts at: 0 1229 1350 Size: 1229 121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 500 532 1350 Size: 500 32 818 Fragments arranged by size: 818 500 32 PpuMI rG'GwC_Cy Cuts at: 0 800 1350 Size: 800 550 PspOMI G'GGCC_C Cuts at: 0 582 1274 1350 Size: 582 692 76 Fragments arranged by size: 692 582 76 PvuII CAG'CTG Cuts at: 0 579 1026 1350 Size: 579 447 324 Fragments arranged by size: 579 447 324 SacI G_AGCT'C Cuts at: 0 832 1350 Size: 832 518 SmlI C'TyrA_G Cuts at: 0 40 823 961 1350 Size: 40 783 138 389 Fragments arranged by size: 783 389 138 40 StyI C'CwwG_G Cuts at: 0 586 1350 Size: 586 764 TatI w'GTAC_w Cuts at: 0 1280 1350 Size: 1280 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 452 484 958 990 1350 Size: 452 32 474 32 360 Fragments arranged by size: 474 452 360 32 32 Tth111I GACn'n_nGTC Cuts at: 0 334 940 1350 Size: 334 606 410 Fragments arranged by size: 606 410 334 XmnI GAAnn'nnTTC Cuts at: 0 314 1350 Size: 314 1036 Enzymes that do cut: AcuI AflII AflIII AloI AlwNI ApaI ApoI BanII BbsI BclI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I BtsI DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I PflMI PpiI PpuMI PspOMI PvuII SacI SmlI StyI TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EagI EarI EcoRI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XcmI XhoI XmaI ZraI